Straw – WGCNA

0.1 Импорт

Импорт phyloseq объекта и библиотек
Датасет - хроносерия разложения соломы - от фактор Day 10ти уровней

library(phyloseq)
library(tidyverse)
library(ggpubr)
library(ampvis2)
library(ANCOMBC)
library(heatmaply)
library(compositions)
library(igraph)
library(WGCNA)
require(DESeq2)
require(phyloseq)

ps.f <- readRDS("psf2")

0.2 functions

#phyloseq object to ampvis2 object
#https://gist.github.com/KasperSkytte/8d0ca4206a66be7ff6d76fc4ab8e66c6

phyloseq_to_ampvis2 <- function(physeq) {
  #check object for class
  if(!any(class(physeq) %in% "phyloseq"))
    stop("physeq object must be of class \"phyloseq\"", call. = FALSE)
  
  #ampvis2 requires taxonomy and abundance table, phyloseq checks for the latter
  if(is.null(physeq@tax_table))
    stop("No taxonomy found in the phyloseq object and is required for ampvis2", call. = FALSE)
  
  #OTUs must be in rows, not columns
  if(phyloseq::taxa_are_rows(physeq))
    abund <- as.data.frame(phyloseq::otu_table(physeq)@.Data)
  else
    abund <- as.data.frame(t(phyloseq::otu_table(physeq)@.Data))
  
  #tax_table is assumed to have OTUs in rows too
  tax <- phyloseq::tax_table(physeq)@.Data
  
  #merge by rownames (OTUs)
  otutable <- merge(
    abund,
    tax,
    by = 0,
    all.x = TRUE,
    all.y = FALSE,
    sort = FALSE
  )
  colnames(otutable)[1] <- "OTU"
  
  #extract sample_data (metadata)
  if(!is.null(physeq@sam_data)) {
    metadata <- data.frame(
      phyloseq::sample_data(physeq),
      row.names = phyloseq::sample_names(physeq), 
      stringsAsFactors = FALSE, 
      check.names = FALSE
    )
    
    #check if any columns match exactly with rownames
    #if none matched assume row names are sample identifiers
    samplesCol <- unlist(lapply(metadata, function(x) {
      identical(x, rownames(metadata))}))
    
    if(any(samplesCol)) {
      #error if a column matched and it's not the first
      if(!samplesCol[[1]])
        stop("Sample ID's must be in the first column in the sample metadata, please reorder", call. = FALSE)
    } else {
      #assume rownames are sample identifiers, merge at the end with name "SampleID"
      if(any(colnames(metadata) %in% "SampleID"))
        stop("A column in the sample metadata is already named \"SampleID\" but does not seem to contain sample ID's", call. = FALSE)
      metadata$SampleID <- rownames(metadata)
      
      #reorder columns so SampleID is the first
      metadata <- metadata[, c(which(colnames(metadata) %in% "SampleID"), 1:(ncol(metadata)-1L)), drop = FALSE]
    }
  } else
    metadata <- NULL
  
  #extract phylogenetic tree, assumed to be of class "phylo"
  if(!is.null(physeq@phy_tree)) {
    tree <- phyloseq::phy_tree(physeq)
  } else
    tree <- NULL
  
  #extract OTU DNA sequences, assumed to be of class "XStringSet"
  if(!is.null(physeq@refseq)) {
    #convert XStringSet to DNAbin using a temporary file (easiest)
    fastaTempFile <- tempfile(pattern = "ampvis2_", fileext = ".fa")
    Biostrings::writeXStringSet(physeq@refseq, filepath = fastaTempFile)
  } else
    fastaTempFile <- NULL
  
  #load as normally with amp_load
  ampvis2::amp_load(
    otutable = otutable,
    metadata = metadata,
    tree = tree,
    fasta = fastaTempFile
  )
}

#variance stabilisation from DESeq2

ps_vst <- function(ps, factor){
  

  
  diagdds = phyloseq_to_deseq2(ps, as.formula(paste( "~", factor)))              
  diagdds = estimateSizeFactors(diagdds, type="poscounts")
  diagdds = estimateDispersions(diagdds, fitType = "local") 
  pst <- varianceStabilizingTransformation(diagdds)
  pst.dimmed <- t(as.matrix(assay(pst))) 
  # pst.dimmed[pst.dimmed < 0.0] <- 0.0
  ps.varstab <- ps
  otu_table(ps.varstab) <- otu_table(pst.dimmed, taxa_are_rows = FALSE) 
  return(ps.varstab)
}

#WGCNA visualisation
#result - list class object with attributes:
# ps - phyloseq object
# amp - ampwis2 object
# heat - heatmap with absalute read numbers
# heat_rel - hetmap with relative abundances
# tree - phylogenetic tree with taxonomy

color_filt <- function(ps, df){
  
      library(tidyverse)
      library(reshape2)
      library(gridExtra)

  
  l = list()
  for (i in levels(df$module)){
    message(i)
    color_name <-  df %>% 
      filter(module == i) %>% 
      pull(asv) %>% 
      unique()
    ps.col <- prune_taxa(color_name, ps)
    amp.col <- phyloseq_to_ampvis2(ps.col)
    heat <- amp_heatmap(amp.col, tax_show = 60, 
              group_by = "Day", 
              tax_aggregate = "OTU",
              tax_add = "Genus", 
              normalise=FALSE, 
              showRemainingTaxa = TRUE)
    
    ps.rel  <-  phyloseq::transform_sample_counts(ps.col, function(x) x / sum(x) * 100)
    amp.r <- phyloseq_to_ampvis2(ps.rel)
    heat.rel <- amp_heatmap(amp.r, tax_show = 60, 
          group_by = "Day", 
          tax_aggregate = "OTU",
          tax_add = "Genus", 
          normalise=FALSE, 
          showRemainingTaxa = TRUE)
    
    tree <- ps.col@phy_tree
    taxa <- as.data.frame(ps.col@tax_table@.Data)
    p1 <- ggtree(tree) + 
              geom_tiplab(size=2, align=TRUE, linesize=.5) +
              theme_tree2()
    
    taxa[taxa == "Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium"] <- "Allorhizobium"
    taxa[taxa == "Burkholderia-Caballeronia-Paraburkholderia"] <- "Burkholderia"
  
    tx <- taxa %>% 
      rownames_to_column("id") %>% 
      mutate(id = factor(id, levels = rev(get_taxa_name(p1)))) %>% 
      dplyr::select(-c(Kingdom, Species, Order)) %>% 
      melt(id.var = 'id')

    p2 <- ggplot(tx, aes(variable, id)) + 
      geom_tile(aes(fill = value), alpha = 0.4) +
      geom_text(aes(label = value), size = 3) +
      theme_bw() + 
      theme(legend.position = "none",
             axis.ticks.x=element_blank(),
             axis.text.y=element_blank(),
             axis.ticks.y=element_blank(),
             axis.title.x = element_blank(),
            axis.title.y = element_blank()) 

    p <- ggpubr::ggarrange(p1, p2, widths = c(0.9, 1))
    l[[i]] <- list("ps" = ps.col, 
                   "amp" = amp.col,
                   "heat" = heat,
                   "heat_rel" = heat.rel,
                   "tree" = p,
                   "taxa" = knitr::kable(taxa))
                    
  }
  return(l)
}


color_filt_broken <- function(ps, df, ps.pruned){
  
      library(tidyverse)
      library(reshape2)
      library(gridExtra)

  
  l = list()
  for (i in levels(df$module)){
    message(i)
    color_name <-  df %>% 
      filter(module == i) %>% 
      pull(asv) %>% 
      unique()
    ps.col <- prune_taxa(color_name, ps)
    ps.col.pruned <- prune_taxa(color_name, ps.pruned)
    amp.col <- phyloseq_to_ampvis2(ps.col)
    heat <- amp_heatmap(amp.col, tax_show = 60, 
              group_by = "Day", 
              tax_aggregate = "OTU",
              tax_add = "Genus", 
              normalise=FALSE, 
              showRemainingTaxa = TRUE)
    
    ps.rel  <-  phyloseq::transform_sample_counts(ps.col, function(x) x / sum(x) * 100)
    amp.r <- phyloseq_to_ampvis2(ps.rel)
    heat.rel <- amp_heatmap(amp.r, tax_show = 60, 
          group_by = "Day", 
          tax_aggregate = "OTU",
          tax_add = "Genus", 
          normalise=FALSE, 
          showRemainingTaxa = TRUE)
    
    tree <- ps.col.pruned@phy_tree
    taxa <- as.data.frame(ps.col.pruned@tax_table@.Data)
    p1 <- ggtree(tree) + 
              geom_tiplab(size=2, align=TRUE, linesize=.5) +
              theme_tree2()
    
    taxa[taxa == "Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium"] <- "Allorhizobium"
    taxa[taxa == "Burkholderia-Caballeronia-Paraburkholderia"] <- "Burkholderia"
  
    tx <- taxa %>% 
      rownames_to_column("id") %>% 
      mutate(id = factor(id, levels = rev(get_taxa_name(p1)))) %>% 
      dplyr::select(-c(Kingdom, Species, Order)) %>% 
      melt(id.var = 'id')

    p2 <- ggplot(tx, aes(variable, id)) + 
      geom_tile(aes(fill = value), alpha = 0.4) +
      geom_text(aes(label = value), size = 3) +
      theme_bw() + 
      theme(legend.position = "none",
             axis.ticks.x=element_blank(),
             axis.text.y=element_blank(),
             axis.ticks.y=element_blank(),
             axis.title.x = element_blank(),
            axis.title.y = element_blank()) 

    p <- ggpubr::ggarrange(p1, p2, widths = c(0.9, 1))
    l[[i]] <- list("ps" = ps.col, 
                   "amp" = amp.col,
                   "heat" = heat,
                   "heat_rel" = heat.rel,
                   "tree" = p,
                   "taxa" = knitr::kable(taxa))
                    
  }
  return(l)
}

detachAllPackages <- function() {

  basic.packages <- c("package:stats","package:graphics","package:grDevices","package:utils","package:datasets","package:methods","package:base")

  package.list <- search()[ifelse(unlist(gregexpr("package:",search()))==1,TRUE,FALSE)]

  package.list <- setdiff(package.list,basic.packages)

  if (length(package.list)>0)  for (package in package.list) detach(package, character.only=TRUE, force = TRUE)

}

plot_alpha_w_toc <- function(ps, group, metric) {
  
  require(phyloseq)
  require(ggplot2)
  
  ps_a <- prune_taxa(taxa_sums(ps) > 0, ps)
  
  er <- estimate_richness(ps_a)
  df_er <- cbind(ps_a@sam_data, er)
  df_er <- df_er %>% select(c(group, metric))
  stat.test <- aov(as.formula(paste0(metric, "~", group)), data = df_er) %>%
    rstatix::tukey_hsd()
  y <-  seq(max(er[[metric]]), length=length(stat.test$p.adj), by=max(er[[metric]]/20))

  plot_richness(ps_a, x=group, measures=metric) + 
    geom_boxplot() +
    geom_point(size=1.2, alpha=0.3) +
    ggpubr::stat_pvalue_manual(
      stat.test, 
      label = "p.adj.signif", 
      y.position = y) +
    theme_light() + 
    scale_color_brewer(palette="Dark2") +
    theme(axis.text.x = element_text(angle = 90),
          axis.title.x=element_blank()) +
    labs(y=paste(metric, "index")) 
}


# standart NMDS plot tool frop phyloseq with some additional aestatics 
# have stress value on plot - may work as fuck
beta_custom_norm_NMDS_elli_w <- function(ps, seed = 7888, normtype="vst", Color="What", Group="Repeat"){
  require(phyloseq)
  require(ggplot2)
  require(ggpubr)
  library(ggforce)
  
  
  ordination.b <- ordinate(ps, "NMDS", "bray")
  mds <- as.data.frame(ordination.b$points)
  p  <-  plot_ordination(ps,
                         ordination.b,
                         type="sample",
                         color = Color,
                         title="NMDS - Bray-Curtis",
                         # title=NULL,
                         axes = c(1,2) ) + 
    theme_bw() + 
    theme(text = element_text(size = 10)) + 
    geom_point(size = 3) +
    annotate("text",
    x=min(mds$MDS1) + abs(min(mds$MDS1))/7,
    y=max(mds$MDS2),
    label=paste0("Stress -- ", round(ordination.b$stress, 3))) +
    geom_mark_ellipse(aes_string(group = Group, label = Group),
                      label.fontsize = 10,
                      label.buffer = unit(2, "mm"),
                      label.minwidth = unit(5, "mm"),
                      con.cap = unit(0.1, "mm"),
                      con.colour='gray') +
    theme(legend.position = "none") +
    ggplot2::scale_fill_viridis_c(option = "H")
  
  return(p)
}


# alpha with aov + tukie post-hock - useless, but it looks pretty good
plot_alpha_w_toc <- function(ps, group, metric) {
  
  require(phyloseq)
  require(ggplot2)
  
  ps_a <- prune_taxa(taxa_sums(ps) > 0, ps)
  
  er <- estimate_richness(ps_a)
  df_er <- cbind(ps_a@sam_data, er)
  df_er <- df_er %>% select(c(group, metric))
  stat.test <- aov(as.formula(paste0(metric, "~", group)), data = df_er) %>%
    rstatix::tukey_hsd()
  y <- seq(max(er[[metric]]), length=length(stat.test$p.adj.signif[stat.test$p.adj.signif != "ns"]), by=max(er[[metric]]/20))
  
  plot_richness(ps_a, x=group, measures=metric) + 
    geom_boxplot() +
    geom_point(size=1.2, alpha=0.3) +
    stat_pvalue_manual(
      stat.test, 
      label = "p.adj.signif", 
      y.position = y,
      hide.ns=TRUE) +
    theme_light() + 
    scale_color_brewer(palette="Dark2") +
    theme(axis.text.x = element_text(angle = 90),
          axis.title.x=element_blank()) +
    labs(y=paste(metric, "index")) 
}

0.3 Alpha

p1 <- plot_alpha_w_toc(ps = ps.f, group = "Day", metric = "Observed")
p2 <- plot_alpha_w_toc(ps = ps.f, group = "Day", metric = "Shannon")
p3 <- plot_alpha_w_toc(ps = ps.f, group = "Day", metric = "InvSimpson")
ggarrange(p1, p2, p3, nrow = 1)

Add Group parameter to metadata -
- early - D01, D03, D05
- middle - D07, D08, D10
- late - D13, D14, D15

sample.data <- ps.f@sam_data %>% 
  data.frame() %>% 
  mutate(Group = if_else(Day %in% c("D01", "D03", "D05"), "early", 
                         if_else(Day %in% c("D07", "D08","D10"), "middle", "late"))) %>% 
  mutate(Group = factor(Group, levels=c("early", "middle","late"))) %>% 
  phyloseq::sample_data()

sample_data(ps.f) <- sample.data

p.observed <- plot_alpha_w_toc(ps = ps.f, group = "Group", metric = c("Observed")) + 
  theme(axis.title.y = element_blank())

p.shannon <- plot_alpha_w_toc(ps = ps.f, group = "Group", metric = c("Shannon")) + 
  theme(axis.title.y = element_blank())

p.simpson <- plot_alpha_w_toc(ps = ps.f, group = "Group", metric = c("InvSimpson")) + 
  theme(axis.title.y = element_blank())

ggpubr::ggarrange(p.observed, p.shannon, p.simpson, ncol = 3)

0.4 beta statistic

0.4.1 permanova

permanova - group significantlly different - dispersion between is more, than inside groups

dist <- phyloseq::distance(ps.f, "bray")
metadata <- as(sample_data(ps.f@sam_data), "data.frame")
vegan::adonis2(dist ~ Group, data = metadata)

## Permutation test for adonis under reduced model
## Terms added sequentially (first to last)
## Permutation: free
## Number of permutations: 999
## 
## vegan::adonis2(formula = dist ~ Group, data = metadata)
##          Df SumOfSqs      R2      F Pr(>F)    
## Group     2   3.2742 0.33893 8.2033  0.001 ***
## Residual 32   6.3861 0.66107                  
## Total    34   9.6604 1.00000                  
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

0.4.2 dynamic of beta diversity

Дистанция брей кертиса по шагам между каждыми днями.   (Например - все D15 против D14)   Может на эту картинку подвесить еще почвенное дыхание?

ps.f.r <- rarefy_even_depth(ps.f, rngseed = 777)

avg.r <- ps.f.r@otu_table %>% 
  as.data.frame() %>%
  vegan::avgdist(10)

avg <- ps.f@otu_table %>% 
  as.data.frame() %>%
  vegan::avgdist(10)

# avg %>%
#   as.matrix() %>%
#   as_tibble(rownames= "sample") %>%
#   pivot_longer(-sample) %>%
#   filter(sample < name) %>%
#   mutate(repeat_a = str_replace(sample, ".*-", ""),
#          repeat_b = str_replace(name, ".*-", ""), 
#          day_a = as.numeric(str_replace(sapply(strsplit(sample, "-"), `[`, 3), "D", "")),
#           day_b = as.numeric(str_replace(sapply(strsplit(name, "-"), `[`, 3), "D", "")),
#          diff = abs(day_a - day_b),
#          early = day_a < 10) %>% 
#   filter(repeat_a == repeat_b & diff < 10) %>%
#   group_by(diff, repeat_a, early) %>%
#   summarize(median = median(value)) %>%
#   ungroup() %>%
#   ggplot(aes(x=diff, y=median, color=early, group=paste0(repeat_a, early))) +
#   geom_line(size=0.25) +
#   geom_smooth(aes(group=early), se=FALSE, size=4) +
#   labs(x="Distance between time points",
#        y="Median Bray-Curtis distance") +
#   scale_x_continuous(breaks=1:9) +
#   scale_color_manual(name=NULL,
#                      breaks=c(TRUE, FALSE),
#                      values=c("blue", "red"),
#                      labels=c("Early", "Late")) +
#   guides(color = guide_legend(override.aes = list(size=1))) +
#   theme_classic()

avg.r %>%
  as.matrix() %>%
  as_tibble(rownames= "sample") %>%
  pivot_longer(-sample) %>%
  filter(sample < name) %>%
  mutate(repeat_a = str_replace(sample, ".*-", ""),
         repeat_b = str_replace(name, ".*-", ""), 
         day_a = as.numeric(str_replace(sapply(strsplit(sample, "-"), `[`, 3), "D", "")),
          day_b = as.numeric(str_replace(sapply(strsplit(name, "-"), `[`, 3), "D", ""))) %>% 
  mutate(day_a = as.numeric(as.factor(day_a) %>% forcats::fct_recode("2" = "3", "3" = "5", "4" = "7", "5" = "8", "6" = "10", "7" = "13", "8" = "14", "9" = "14", "10" = "15")),
         day_b = as.numeric(as.factor(day_b) %>% forcats::fct_recode("2" = "3", "3" = "5", "4" = "7", "5" = "8", "6" = "10", "7" = "12", "8" = "13", "9" = "14", "10" = "15")),
         diff = abs(day_a - day_b)) %>% 
  filter(diff == 1) %>% 
  mutate(day_b = as.factor(day_b) %>% forcats::fct_recode("D03" = "2", "D05" = "3","D07" = "4",  "D08" = "5", "D10" = "6", "D13" = "7", "D14" = "8", "D15" = "10", "D12" = "7")) %>% 
  ggplot(aes(x=day_b, y=value)) +
    geom_boxplot() +
  theme_bw() +
  labs(x="Time points",
       y="Bray-Curtis distance")

0.4.3 basic NMDS plot

По картинке кажется что происходит постепенное замедление изменений -
но из предыдущей картинки следует, что это не так - есть провал между 7-8-10,
но в среднем точки различаются довольно одинакого.

beta_custom_norm_NMDS_elli_w(ps.f, C="Group", G="Day")

## Square root transformation
## Wisconsin double standardization
## Run 0 stress 0.1048129 
## Run 1 stress 0.1372731 
## Run 2 stress 0.1318495 
## Run 3 stress 0.1048129 
## ... Procrustes: rmse 0.00004856956  max resid 0.0001949092 
## ... Similar to previous best
## Run 4 stress 0.1048129 
## ... New best solution
## ... Procrustes: rmse 0.00001842645  max resid 0.00006948497 
## ... Similar to previous best
## Run 5 stress 0.1426236 
## Run 6 stress 0.1048204 
## ... Procrustes: rmse 0.001770292  max resid 0.007527799 
## ... Similar to previous best
## Run 7 stress 0.1064532 
## Run 8 stress 0.1064733 
## Run 9 stress 0.1048204 
## ... Procrustes: rmse 0.001767644  max resid 0.007510715 
## ... Similar to previous best
## Run 10 stress 0.1064532 
## Run 11 stress 0.1318495 
## Run 12 stress 0.1064733 
## Run 13 stress 0.1064733 
## Run 14 stress 0.1460032 
## Run 15 stress 0.1048204 
## ... Procrustes: rmse 0.001769848  max resid 0.007525048 
## ... Similar to previous best
## Run 16 stress 0.1064532 
## Run 17 stress 0.1372728 
## Run 18 stress 0.1048129 
## ... Procrustes: rmse 0.000002711789  max resid 0.0000100797 
## ... Similar to previous best
## Run 19 stress 0.1460029 
## Run 20 stress 0.146003 
## *** Solution reached

0.5 Splitting the dataset

Разделим датасет на две группы

1-я - в более чем 10% образцов должно быть хотя бы 10 ридов - эта группа пойдет в анализ далее

ps.inall <- phyloseq::filter_taxa(ps.f, function(x) sum(x > 10) > (0.1*length(x)), TRUE)

amp.inall <- phyloseq_to_ampvis2(ps.inall)
amp_heatmap(amp.inall,
            tax_show = 40,
            group_by = "Day",
            tax_aggregate = "OTU",
            tax_add = "Genus",
            normalise=FALSE,
            showRemainingTaxa = TRUE)

Вторая - оставшиеся, но более 100 ридов по всем образцам(далее - вылетающие мажоры(ВМ))
Остальные филотипы выкидываем из анализа

ps.exl  <- phyloseq::filter_taxa(ps.f, function(x) sum(x > 10) < (0.1*length(x)), TRUE)
ps.exl  <- prune_taxa(taxa_sums(ps.exl) > 100, ps.exl)
amp.exl <- phyloseq_to_ampvis2(ps.exl)
amp_heatmap(amp.exl,
            tax_show = 40,
            group_by = "Day",
            tax_aggregate = "OTU",
            tax_add = "Genus",
            normalise=FALSE,
            showRemainingTaxa = TRUE)

То же, но c относительной представленностью.

ps.per  <-  phyloseq::transform_sample_counts(ps.f, function(x) x / sum(x) * 100) 
ps.exl.taxa <- taxa_names(ps.exl)
ps.per.exl <- prune_taxa(ps.exl.taxa, ps.per)
amp.exl.r <- phyloseq_to_ampvis2(ps.per.exl)

amp_heatmap(amp.exl.r, tax_show = 60, 
      group_by = "Day", 
      tax_aggregate = "OTU",
      tax_add = "Genus", 
      round =  2, 
      normalise=FALSE, 
      showRemainingTaxa = TRUE)

amp_heatmap(amp.exl.r, tax_show = 60, 
      group_by = "Day", 
      tax_aggregate = "OTU",
      tax_add = "Genus", 
      round =  2, 
      normalise=FALSE, )

ВМ объединенные по родам. Относительная представленность.

amp_heatmap(amp.exl.r,
            tax_show = 30, 
            group_by = "Day", 
            tax_aggregate = "Genus",
            tax_add = "Phylum",
            tax_class = "Proteobacteria",
            round =  2,
            normalise=FALSE, 
            showRemainingTaxa = TRUE)

Если посмотреть, если как распределяются ВМ по дням - похоже распределение ВМ связано не только с особенностью отдельных мешочков, но и с чисто техническими особенностями - вылетающие значения вылетают в основном не с биологическими повторностями, а с техническими(красная линия - выбрана визуально)

p_box <- phyloseq::sample_sums(ps.per.exl) %>% 
  as.data.frame(col.names = "values") %>% 
  setNames(., nm = "values") %>% 
  rownames_to_column("samples") %>% 
  mutate(Day = sapply(strsplit(samples, "-"), `[`, 3)) %>% 
  ggplot(aes(x=Day, y=values, color=Day, fill = Day)) +
  geom_boxplot(aes(color=Day, fill = Day)) +
  geom_point(color = "black", position = position_dodge(width=0.2)) +
  geom_hline(yintercept = 10, colour = "red") +
  theme_bw() +
  theme(legend.position = "none")
     
p_box <- p_box + viridis::scale_color_viridis(option = "H", discrete = TRUE, direction=1, begin=0.1, end = 0.9, alpha = 0.5)

p_box + viridis::scale_fill_viridis(option = "H", discrete = TRUE, direction=1, begin=0.1, end = 0.9, alpha = 0.3)

0.6 WGCNA

0.6.1 clr

после clr нормализации / выглядит отвратительно попробуем нормализацию vst из DESeq2

otu.inall <- as.data.frame(ps.inall@otu_table@.Data) 
ps.inall.clr <- ps.inall
otu_table(ps.inall.clr) <- phyloseq::otu_table(compositions::clr(otu.inall), taxa_are_rows = FALSE)
data <- ps.inall.clr@otu_table@.Data %>% 
  as.data.frame()
rownames(data) <- as.character(ps.inall.clr@sam_data$Description)
powers <-  c(c(1:10), seq(from = 12, to=30, by=1))
sft <-  pickSoftThreshold(data, powerVector = powers, verbose = 5, networkType = "signed")

## pickSoftThreshold: will use block size 338.
##  pickSoftThreshold: calculating connectivity for given powers...
##    ..working on genes 1 through 338 of 338

## Warning: executing %dopar% sequentially: no parallel backend registered

##    Power SFT.R.sq  slope truncated.R.sq  mean.k. median.k.  max.k.
## 1      1    0.185  23.60         0.8790 168.0000 168.00000 172.000
## 2      2    0.199 -15.50         0.8970  87.6000  87.20000  95.100
## 3      3    0.518 -13.10         0.8580  47.3000  46.80000  55.900
## 4      4    0.390  -6.46         0.8050  26.5000  26.00000  34.500
## 5      5    0.262  -3.46         0.7820  15.4000  14.90000  22.300
## 6      6    0.232  -2.30         0.7770   9.2300   8.78000  15.000
## 7      7    0.251  -1.87         0.6990   5.7300   5.36000  10.500
## 8      8    0.319  -1.75         0.7570   3.6800   3.41000   7.620
## 9      9    0.527  -2.07         0.7770   2.4400   2.20000   5.890
## 10    10    0.648  -2.28         0.8490   1.6600   1.47000   4.690
## 11    12    0.827  -2.41         0.8970   0.8420   0.69300   3.190
## 12    13    0.793  -2.38         0.7930   0.6220   0.49000   2.700
## 13    14    0.277  -3.97         0.0914   0.4690   0.35500   2.320
## 14    15    0.282  -3.83         0.1000   0.3610   0.26100   2.010
## 15    16    0.894  -2.17         0.9030   0.2820   0.19800   1.770
## 16    17    0.905  -2.10         0.9080   0.2240   0.15000   1.560
## 17    18    0.922  -2.04         0.9240   0.1810   0.11500   1.390
## 18    19    0.927  -2.01         0.9230   0.1480   0.08820   1.250
## 19    20    0.938  -1.93         0.9370   0.1220   0.06780   1.130
## 20    21    0.946  -1.86         0.9470   0.1020   0.05260   1.020
## 21    22    0.315  -3.12         0.2220   0.0856   0.04140   0.926
## 22    23    0.303  -3.96         0.1560   0.0728   0.03270   0.844
## 23    24    0.307  -2.92         0.1940   0.0623   0.02590   0.772
## 24    25    0.303  -2.84         0.1800   0.0538   0.02060   0.708
## 25    26    0.921  -1.63         0.8990   0.0467   0.01650   0.657
## 26    27    0.892  -1.61         0.8650   0.0408   0.01320   0.622
## 27    28    0.155  -1.93        -0.0202   0.0359   0.01040   0.591
## 28    29    0.199  -2.77        -0.0228   0.0317   0.00841   0.563
## 29    30    0.199  -2.69        -0.0203   0.0282   0.00684   0.537

plot(sft$fitIndices[,1], -sign(sft$fitIndices[,3])*sft$fitIndices[,2], xlab="Soft Threshold (power)",ylab="Scale Free Topology Model Fit,signed R^2",type="n", main = paste("Scale independence"))
text(sft$fitIndices[,1], -sign(sft$fitIndices[,3])*sft$fitIndices[,2], labels=powers,cex=0.9,col="red")
abline(h=0.9,col="salmon")

0.6.2 vst

после vst нормализации

ps.varstab <- ps_vst(ps.inall, "Day")

data2 <- ps.varstab@otu_table@.Data %>% 
  as.data.frame()

rownames(data2) <- as.character(ps.varstab@sam_data$Description)
powers <-  c(seq(from = 1, to=10, by=0.5), seq(from = 11, to=20, by=1))
sft2 <-  pickSoftThreshold(data2, powerVector = powers, verbose = 5, networkType = "signed hybrid")

## pickSoftThreshold: will use block size 338.
##  pickSoftThreshold: calculating connectivity for given powers...
##    ..working on genes 1 through 338 of 338
##    Power SFT.R.sq slope truncated.R.sq mean.k. median.k. max.k.
## 1    1.0    0.409 -1.05          0.704  50.100  45.60000  97.80
## 2    1.5    0.578 -1.07          0.895  30.900  27.00000  71.20
## 3    2.0    0.723 -1.15          0.942  20.300  17.90000  53.60
## 4    2.5    0.767 -1.24          0.871  14.000  12.00000  41.70
## 5    3.0    0.809 -1.31          0.909  10.000   7.86000  33.40
## 6    3.5    0.872 -1.31          0.958   7.380   5.39000  27.30
## 7    4.0    0.888 -1.30          0.968   5.580   3.76000  22.60
## 8    4.5    0.858 -1.36          0.910   4.320   2.67000  19.00
## 9    5.0    0.892 -1.36          0.943   3.400   1.94000  16.20
## 10   5.5    0.899 -1.38          0.954   2.720   1.41000  13.90
## 11   6.0    0.896 -1.39          0.962   2.210   1.07000  12.10
## 12   6.5    0.897 -1.33          0.962   1.820   0.84700  10.60
## 13   7.0    0.910 -1.30          0.970   1.520   0.65400   9.30
## 14   7.5    0.914 -1.31          0.966   1.280   0.51500   8.25
## 15   8.0    0.913 -1.28          0.954   1.090   0.41500   7.36
## 16   8.5    0.905 -1.28          0.937   0.938   0.34000   6.59
## 17   9.0    0.895 -1.30          0.913   0.813   0.27800   5.99
## 18   9.5    0.878 -1.33          0.900   0.709   0.23200   5.49
## 19  10.0    0.886 -1.34          0.894   0.624   0.19100   5.07
## 20  11.0    0.911 -1.31          0.928   0.491   0.12500   4.35
## 21  12.0    0.890 -1.29          0.894   0.396   0.08590   3.79
## 22  13.0    0.910 -1.26          0.918   0.325   0.06120   3.33
## 23  14.0    0.882 -1.25          0.856   0.272   0.04420   2.95
## 24  15.0    0.889 -1.19          0.864   0.230   0.03230   2.63
## 25  16.0    0.913 -1.18          0.893   0.198   0.02240   2.35
## 26  17.0    0.930 -1.16          0.912   0.172   0.01620   2.20
## 27  18.0    0.955 -1.19          0.947   0.151   0.01190   2.09
## 28  19.0    0.908 -1.21          0.896   0.134   0.00896   1.99
## 29  20.0    0.916 -1.21          0.906   0.120   0.00649   1.91

plot(sft2$fitIndices[,1], -sign(sft2$fitIndices[,3])*sft2$fitIndices[,2], xlab="Soft Threshold (power)",ylab="Scale Free Topology Model Fit,signed R^2",type="n", main = paste("Scale independence"))
text(sft2$fitIndices[,1], -sign(sft2$fitIndices[,3])*sft2$fitIndices[,2], labels=powers,cex=0.9,col="red")
abline(h=0.9,col="salmon")

# WGCNA, as old and not supported
detachAllPackages()
library(WGCNA)


net3 <- WGCNA::blockwiseModules(data2,
                          power=5.5,
                          TOMType="signed",
                          networkType="signed hybrid",
                          nThreads=0)


mergedColors2 <-  WGCNA::labels2colors(net3$colors, colorSeq = c("salmon", "darkgreen", "cyan", "red", "blue", "plum"))

plotDendroAndColors(
  net3$dendrograms[[1]],
  mergedColors2[net3$blockGenes[[1]]],
  "Module colors",
  dendroLabels = FALSE,
  hang = 0.03,
  addGuide = TRUE,
  guideHang = 0.05)

0.6.2.1 Импорт 2

library(phyloseq)
library(tidyverse)
library(ggpubr)
library(ampvis2)
library(heatmaply)
library(WGCNA)
library(phyloseq)
library(ggtree)
library(tidyverse)
library(KneeArrower)

modules_of_interest = mergedColors2 %>% 
  unique()

module_df <- data.frame(
  asv = names(net3$colors),
  colors = mergedColors2
)

# module_df[module_df == "yellow"] <- "salmon"

submod <-  module_df %>%
  subset(colors %in% modules_of_interest)

row.names(module_df) = module_df$asv

subexpr = as.data.frame(t(data2))[submod$asv,]

submod_df <- data.frame(subexpr) %>%
  mutate(
    asv = row.names(.)
  ) %>%
  pivot_longer(-asv) %>%
  mutate(
    module = module_df[asv,]$colors
  )

submod_df <- submod_df %>% 
  mutate(name = gsub("\\_.*","",submod_df$name)) %>% 
  group_by(name, asv) %>% 
  summarise(value = mean(value), asv = asv, module = module) %>% 
  relocate(c(asv, name, value, module)) %>% 
  ungroup() %>% 
  mutate(module = as.factor(module))

p <- submod_df %>% 
  ggplot(., aes(x=name, y=value, group=asv)) +
  geom_line(aes(color = module),
            alpha = 0.2) +
  theme_bw() +
  theme(
    axis.text.x = element_text(angle = 90),
    legend.position = "none") +
  facet_grid(rows = vars(module)) +
  labs(x = "treatment",
       y = "normalized expression")

p + scale_color_manual(values = levels(submod_df$module))

0.6.2.2 ordination

UNIFRAC - колбаска сужается

ps.inall.col <- ps.inall
df <- module_df %>% 
  rename("id" = "asv")
df <- df %>% 
  dplyr::select(-"id") %>% 
  mutate(colors = as.factor(colors))
taxa <- as.data.frame(ps.inall@tax_table@.Data)
tx <- cbind(taxa, df)
tx$colors <- factor(tx$colors, levels = c("salmon", "darkgreen", "cyan", "red", "blue", "plum"))
tax_table(ps.inall.col) <- tax_table(as.matrix(tx))
ord <- ordinate(ps.inall.col, "NMDS", "unifrac")

## Run 0 stress 0.0685455 
## Run 1 stress 0.05568715 
## ... New best solution
## ... Procrustes: rmse 0.04076806  max resid 0.2160826 
## Run 2 stress 0.06784429 
## Run 3 stress 0.05628488 
## Run 4 stress 0.05568719 
## ... Procrustes: rmse 0.0001769095  max resid 0.0006062451 
## ... Similar to previous best
## Run 5 stress 0.07937976 
## Run 6 stress 0.07544596 
## Run 7 stress 0.05628489 
## Run 8 stress 0.05568719 
## ... Procrustes: rmse 0.00002559041  max resid 0.00008856774 
## ... Similar to previous best
## Run 9 stress 0.05568712 
## ... New best solution
## ... Procrustes: rmse 0.00002106782  max resid 0.00006934972 
## ... Similar to previous best
## Run 10 stress 0.06740137 
## Run 11 stress 0.06740136 
## Run 12 stress 0.06784421 
## Run 13 stress 0.06784436 
## Run 14 stress 0.06854546 
## Run 15 stress 0.07953205 
## Run 16 stress 0.05568719 
## ... Procrustes: rmse 0.00004255519  max resid 0.0001401031 
## ... Similar to previous best
## Run 17 stress 0.06740138 
## Run 18 stress 0.06728216 
## Run 19 stress 0.05568718 
## ... Procrustes: rmse 0.0000373923  max resid 0.0001312747 
## ... Similar to previous best
## Run 20 stress 0.07888487 
## *** Solution reached

plot_ordination(ps.inall.col, ord, type = "species", color = "colors") + 
  scale_color_manual(values =  c("salmon", "darkgreen", "cyan", "red", "blue", "plum")) +
  theme_bw() +
  theme(legend.position = "none")

Bray - колбаска равномерна
Поздние стадии слева - при этом ранние кластеры накладываются, поздние разделены Что влияет на ось2? Явно есть какой-то паттерн.

ord <- ordinate(ps.inall.col, "NMDS", "bray")

## Square root transformation
## Wisconsin double standardization
## Run 0 stress 0.09036937 
## Run 1 stress 0.0903694 
## ... Procrustes: rmse 0.00001927814  max resid 0.00007086537 
## ... Similar to previous best
## Run 2 stress 0.09024752 
## ... New best solution
## ... Procrustes: rmse 0.003386048  max resid 0.01505777 
## Run 3 stress 0.09036938 
## ... Procrustes: rmse 0.003390695  max resid 0.01505826 
## Run 4 stress 0.09036937 
## ... Procrustes: rmse 0.003386035  max resid 0.01507802 
## Run 5 stress 0.09036937 
## ... Procrustes: rmse 0.003386432  max resid 0.01505862 
## Run 6 stress 0.1257473 
## Run 7 stress 0.09036937 
## ... Procrustes: rmse 0.003385691  max resid 0.01507976 
## Run 8 stress 0.1268919 
## Run 9 stress 0.09036944 
## ... Procrustes: rmse 0.003410952  max resid 0.01523599 
## Run 10 stress 0.09024752 
## ... New best solution
## ... Procrustes: rmse 0.00003844186  max resid 0.0001638862 
## ... Similar to previous best
## Run 11 stress 0.09036937 
## ... Procrustes: rmse 0.003380784  max resid 0.0150593 
## Run 12 stress 0.09036938 
## ... Procrustes: rmse 0.003379871  max resid 0.01508219 
## Run 13 stress 0.09036939 
## ... Procrustes: rmse 0.003385498  max resid 0.01512324 
## Run 14 stress 0.1358024 
## Run 15 stress 0.09024754 
## ... Procrustes: rmse 0.00003794727  max resid 0.0001665169 
## ... Similar to previous best
## Run 16 stress 0.1258672 
## Run 17 stress 0.1358024 
## Run 18 stress 0.09036937 
## ... Procrustes: rmse 0.003376715  max resid 0.01503468 
## Run 19 stress 0.126985 
## Run 20 stress 0.1360016 
## *** Solution reached

plot_ordination(ps.inall.col, ord, type = "species", color = "colors") + 
  scale_color_manual(values =  c("salmon", "darkgreen", "cyan", "red", "blue", "plum")) +
  theme_bw() +
  theme(legend.position = "none")

0.6.2.3 visualisation

Далее идут одинаковые картинки по всем группам.

l_vst <- color_filt(ps.inall, submod_df)
l_vst

$blue \(blue\)ps phyloseq-class experiment-level object otu_table() OTU Table: [ 86 taxa and 35 samples ] sample_data() Sample Data: [ 35 samples by 3 sample variables ] tax_table() Taxonomy Table: [ 86 taxa by 7 taxonomic ranks ] phy_tree() Phylogenetic Tree: [ 86 tips and 85 internal nodes ] refseq() DNAStringSet: [ 86 reference sequences ]

\(blue\)amp ampvis2 object with 5 elements. Summary of OTU table: Samples OTUs Total#Reads Min#Reads Max#Reads Median#Reads 35 86 89493 0 11387 1819 Avg#Reads 2556.94

Assigned taxonomy: Kingdom Phylum Class Order Family Genus Species 86(100%) 86(100%) 86(100%) 85(98.84%) 79(91.86%) 50(58.14%) 7(8.14%)

Metadata variables: 4 SampleID, Day, Description, Group

\(blue\)heat \(blue\)heat_rel \(blue\)tree \(blue\)taxa

	Kingdom	Phylum	Class	Order	Family	Genus	Species
Seq314	Bacteria	Cyanobacteria	Vampirivibrionia	Obscuribacterales	Obscuribacteraceae	NA	NA
Seq23	Bacteria	Cyanobacteria	Vampirivibrionia	Obscuribacterales	Obscuribacteraceae	NA	NA
Seq65	Bacteria	Proteobacteria	Alphaproteobacteria	Sphingomonadales	Sphingomonadaceae	Sphingomonas	NA
Seq188	Bacteria	Proteobacteria	Alphaproteobacteria	Reyranellales	Reyranellaceae	Reyranella	soli
Seq334	Bacteria	Proteobacteria	Alphaproteobacteria	Reyranellales	Reyranellaceae	Reyranella	NA
Seq75	Bacteria	Proteobacteria	Alphaproteobacteria	Reyranellales	Reyranellaceae	Reyranella	NA
Seq167	Bacteria	Proteobacteria	Alphaproteobacteria	Reyranellales	Reyranellaceae	Reyranella	massiliensis
Seq273	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Kaistiaceae	Kaistia	NA
Seq131	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	Pseudolabrys	NA
Seq138	Bacteria	Proteobacteria	Alphaproteobacteria	Micropepsales	Micropepsaceae	NA	NA
Seq104	Bacteria	Proteobacteria	Alphaproteobacteria	Micropepsales	Micropepsaceae	NA	NA
Seq129	Bacteria	Proteobacteria	Alphaproteobacteria	Sphingomonadales	Sphingomonadaceae	Altererythrobacter	NA
Seq28	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Devosiaceae	Devosia	NA
Seq360	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	Mesorhizobium	NA
Seq292	Bacteria	Proteobacteria	Alphaproteobacteria	Caulobacterales	Caulobacteraceae	NA	NA
Seq166	Bacteria	Proteobacteria	Alphaproteobacteria	Caulobacterales	Hyphomonadaceae	Hirschia	NA
Seq39	Bacteria	Gemmatimonadota	Gemmatimonadetes	Gemmatimonadales	Gemmatimonadaceae	NA	NA
Seq30	Bacteria	Actinobacteriota	Thermoleophilia	Solirubrobacterales	Solirubrobacteraceae	Conexibacter	NA
Seq151	Bacteria	Actinobacteriota	Thermoleophilia	Solirubrobacterales	67-14	NA	NA
Seq250	Bacteria	Actinobacteriota	Thermoleophilia	Solirubrobacterales	67-14	NA	NA
Seq135	Bacteria	Myxococcota	Polyangia	Polyangiales	BIrii41	NA	NA
Seq46	Bacteria	Myxococcota	Polyangia	Polyangiales	BIrii41	NA	NA
Seq35	Bacteria	Myxococcota	Polyangia	Polyangiales	BIrii41	NA	NA
Seq106	Bacteria	Myxococcota	Polyangia	Polyangiales	Sandaracinaceae	NA	NA
Seq345	Bacteria	Bdellovibrionota	Oligoflexia	0319-6G20	NA	NA	NA
Seq198	Bacteria	Bdellovibrionota	Bdellovibrionia	Bdellovibrionales	Bdellovibrionaceae	Bdellovibrio	NA
Seq97	Bacteria	Bdellovibrionota	Bdellovibrionia	Bdellovibrionales	Bdellovibrionaceae	Bdellovibrio	NA
Seq183	Bacteria	Dependentiae	Babeliae	Babeliales	UBA12409	NA	NA
Seq424	Bacteria	Actinobacteriota	Actinobacteria	Propionibacteriales	Nocardioidaceae	Kribbella	NA
Seq136	Bacteria	Actinobacteriota	Acidimicrobiia	Microtrichales	Iamiaceae	Iamia	NA
Seq174	Bacteria	Actinobacteriota	Acidimicrobiia	Microtrichales	Iamiaceae	Iamia	NA
Seq43	Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Microbacteriaceae	Galbitalea	NA
Seq420	Bacteria	Actinobacteriota	Actinobacteria	Micromonosporales	Micromonosporaceae	Luedemannella	NA
Seq127	Bacteria	Actinobacteriota	Actinobacteria	Micromonosporales	Micromonosporaceae	Dactylosporangium	NA
Seq288	Bacteria	Actinobacteriota	Actinobacteria	Streptomycetales	Streptomycetaceae	NA	NA
Seq449	Bacteria	Actinobacteriota	Actinobacteria	Propionibacteriales	Propionibacteriaceae	Jiangella	NA
Seq91	Bacteria	Bacteroidota	Bacteroidia	Bacteroidales	NA	NA	NA
Seq149	Bacteria	Bacteroidota	Bacteroidia	Bacteroidetes VC2.1 Bac22	NA	NA	NA
Seq169	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	Sphingobacteriaceae	Mucilaginibacter	calamicampi
Seq81	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	NS11-12 marine group	NA	NA
Seq211	Bacteria	Bacteroidota	Bacteroidia	NA	NA	NA	NA
Seq119	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	env.OPS 17	NA	NA
Seq77	Bacteria	Spirochaetota	Spirochaetia	Spirochaetales	Spirochaetaceae	Salinispira	NA
Seq175	Bacteria	Spirochaetota	Spirochaetia	Spirochaetales	Spirochaetaceae	Spirochaeta 2	NA
Seq305	Bacteria	Chloroflexi	Anaerolineae	Anaerolineales	Anaerolineaceae	NA	NA
Seq379	Bacteria	Chloroflexi	Chloroflexia	Chloroflexales	Roseiflexaceae	NA	NA
Seq165	Bacteria	Chloroflexi	Chloroflexia	Chloroflexales	Roseiflexaceae	NA	NA
Seq327	Bacteria	Chloroflexi	Chloroflexia	Thermomicrobiales	JG30-KF-CM45	NA	NA
Seq252	Bacteria	Armatimonadota	Fimbriimonadia	Fimbriimonadales	Fimbriimonadaceae	NA	NA
Seq382	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Pedosphaerales	Pedosphaeraceae	NA	NA
Seq123	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Opitutales	Opitutaceae	Lacunisphaera	limnophila
Seq124	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Chthoniobacterales	Terrimicrobiaceae	Terrimicrobium	NA
Seq325	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Chthoniobacterales	Chthoniobacteraceae	LD29	NA
Seq272	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Verrucomicrobiales	Verrucomicrobiaceae	Roseimicrobium	gellanilyticum
Seq402	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Verrucomicrobiales	Rubritaleaceae	Luteolibacter	NA
Seq322	Bacteria	Planctomycetota	Planctomycetes	Pirellulales	Pirellulaceae	NA	NA
Seq356	Bacteria	Planctomycetota	Planctomycetes	Pirellulales	Pirellulaceae	NA	NA
Seq259	Bacteria	Planctomycetota	Planctomycetes	Pirellulales	Pirellulaceae	Pir4 lineage	NA
Seq279	Bacteria	Planctomycetota	Planctomycetes	Pirellulales	Pirellulaceae	Pir4 lineage	NA
Seq214	Bacteria	Planctomycetota	Planctomycetes	Planctomycetales	Rubinisphaeraceae	SH-PL14	NA
Seq209	Bacteria	Planctomycetota	Planctomycetes	Planctomycetales	Schlesneriaceae	Schlesneria	NA
Seq229	Bacteria	Acidobacteriota	Vicinamibacteria	Vicinamibacterales	Vicinamibacteraceae	NA	NA
Seq275	Bacteria	Acidobacteriota	Vicinamibacteria	Vicinamibacterales	Vicinamibacteraceae	NA	NA
Seq247	Bacteria	Acidobacteriota	Vicinamibacteria	Vicinamibacterales	NA	NA	NA
Seq316	Bacteria	Bacteroidota	Kapabacteria	Kapabacteriales	NA	NA	NA
Seq371	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq12	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq153	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq326	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq10	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	NA	NA
Seq82	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Terrimonas	NA
Seq462	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Pseudoflavitalea	NA
Seq150	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Flavitalea	NA
Seq290	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Edaphobaculum	NA
Seq339	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Edaphobaculum	NA
Seq120	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Taibaiella	NA
Seq233	Bacteria	Proteobacteria	Gammaproteobacteria	Pseudomonadales	211ds20	NA	NA
Seq133	Bacteria	Proteobacteria	Gammaproteobacteria	Steroidobacterales	Steroidobacteraceae	Steroidobacter	flavus
Seq349	Bacteria	Proteobacteria	Gammaproteobacteria	Steroidobacterales	Steroidobacteraceae	Steroidobacter	NA
Seq315	Bacteria	Proteobacteria	Gammaproteobacteria	Gammaproteobacteria Incertae Sedis	Unknown Family	Acidibacter	NA
Seq57	Bacteria	Proteobacteria	Gammaproteobacteria	Gammaproteobacteria Incertae Sedis	Unknown Family	Acidibacter	NA
Seq96	Bacteria	Proteobacteria	Gammaproteobacteria	R7C24	NA	NA	NA
Seq170	Bacteria	Proteobacteria	Gammaproteobacteria	Gammaproteobacteria Incertae Sedis	Unknown Family	Acidibacter	NA
Seq94	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Rhodanobacteraceae	Dokdonella	ginsengisoli
Seq114	Bacteria	Proteobacteria	Gammaproteobacteria	Diplorickettsiales	Diplorickettsiaceae	NA	NA
Seq235	Bacteria	Proteobacteria	Gammaproteobacteria	Legionellales	Legionellaceae	Legionella	NA

$cyan \(cyan\)ps phyloseq-class experiment-level object otu_table() OTU Table: [ 30 taxa and 35 samples ] sample_data() Sample Data: [ 35 samples by 3 sample variables ] tax_table() Taxonomy Table: [ 30 taxa by 7 taxonomic ranks ] phy_tree() Phylogenetic Tree: [ 30 tips and 29 internal nodes ] refseq() DNAStringSet: [ 30 reference sequences ]

\(cyan\)amp ampvis2 object with 5 elements. Summary of OTU table: Samples OTUs Total#Reads Min#Reads Max#Reads Median#Reads 35 30 176073 336 15108 4911 Avg#Reads 5030.66

Assigned taxonomy: Kingdom Phylum Class Order Family Genus Species 30(100%) 30(100%) 30(100%) 30(100%) 30(100%) 29(96.67%) 2(6.67%)

Metadata variables: 4 SampleID, Day, Description, Group

\(cyan\)heat \(cyan\)heat_rel \(cyan\)tree \(cyan\)taxa

	Kingdom	Phylum	Class	Order	Family	Genus	Species
Seq98	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Burkholderiaceae	Burkholderia	telluris
Seq64	Bacteria	Proteobacteria	Alphaproteobacteria	Azospirillales	Inquilinaceae	Inquilinus	ginsengisoli
Seq4	Bacteria	Proteobacteria	Alphaproteobacteria	Azospirillales	Inquilinaceae	Inquilinus	NA
Seq244	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	Pseudorhodoplanes	NA
Seq11	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	Bradyrhizobium	NA
Seq74	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	Bradyrhizobium	NA
Seq22	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	Starkeya	NA
Seq45	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	Allorhizobium	NA
Seq9	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	Allorhizobium	NA
Seq29	Bacteria	Myxococcota	Polyangia	Nannocystales	Nannocystaceae	Nannocystis	NA
Seq239	Bacteria	Myxococcota	Polyangia	Polyangiales	Polyangiaceae	Labilithrix	NA
Seq50	Bacteria	Actinobacteriota	Actinobacteria	Corynebacteriales	Mycobacteriaceae	Mycobacterium	NA
Seq34	Bacteria	Actinobacteriota	Actinobacteria	Streptosporangiales	Streptosporangiaceae	Herbidospora	NA
Seq56	Bacteria	Firmicutes	Bacilli	Paenibacillales	Paenibacillaceae	Paenibacillus	NA
Seq25	Bacteria	Firmicutes	Bacilli	Bacillales	Bacillaceae	Terribacillus	NA
Seq3	Bacteria	Firmicutes	Bacilli	Bacillales	Bacillaceae	Bacillus	NA
Seq5	Bacteria	Firmicutes	Bacilli	Bacillales	Bacillaceae	Bacillus	NA
Seq13	Bacteria	Firmicutes	Bacilli	Bacillales	Planococcaceae	NA	NA
Seq7	Bacteria	Firmicutes	Bacilli	Bacillales	Planococcaceae	Solibacillus	NA
Seq19	Bacteria	Firmicutes	Bacilli	Bacillales	Planococcaceae	Solibacillus	NA
Seq17	Bacteria	Planctomycetota	Planctomycetes	Isosphaerales	Isosphaeraceae	Singulisphaera	NA
Seq324	Bacteria	Acidobacteriota	Acidobacteriae	Acidobacteriales	Acidobacteriaceae (Subgroup 1)	Edaphobacter	NA
Seq16	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq6	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq1	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq109	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq49	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Taibaiella	NA
Seq73	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Spirosomaceae	Dyadobacter	NA
Seq15	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Rhodanobacteraceae	Luteibacter	NA
Seq26	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Xanthomonadaceae	Luteimonas	NA

$darkgreen \(darkgreen\)ps phyloseq-class experiment-level object otu_table() OTU Table: [ 44 taxa and 35 samples ] sample_data() Sample Data: [ 35 samples by 3 sample variables ] tax_table() Taxonomy Table: [ 44 taxa by 7 taxonomic ranks ] phy_tree() Phylogenetic Tree: [ 44 tips and 43 internal nodes ] refseq() DNAStringSet: [ 44 reference sequences ]

\(darkgreen\)amp ampvis2 object with 5 elements. Summary of OTU table: Samples OTUs Total#Reads Min#Reads Max#Reads Median#Reads 35 44 12360 0 2468 65 Avg#Reads 353.14

Assigned taxonomy: Kingdom Phylum Class Order Family Genus Species 44(100%) 44(100%) 44(100%) 43(97.73%) 40(90.91%) 24(54.55%) 3(6.82%)

Metadata variables: 4 SampleID, Day, Description, Group

\(darkgreen\)heat \(darkgreen\)heat_rel \(darkgreen\)tree \(darkgreen\)taxa

	Kingdom	Phylum	Class	Order	Family	Genus	Species
Seq342	Archaea	Crenarchaeota	Nitrososphaeria	Nitrososphaerales	Nitrososphaeraceae	NA	NA
Seq196	Archaea	Crenarchaeota	Nitrososphaeria	Nitrososphaerales	Nitrososphaeraceae	Candidatus Nitrocosmicus	NA
Seq276	Archaea	Crenarchaeota	Nitrososphaeria	Nitrososphaerales	Nitrososphaeraceae	NA	NA
Seq419	Archaea	Crenarchaeota	Nitrososphaeria	Nitrososphaerales	Nitrososphaeraceae	NA	NA
Seq454	Bacteria	Proteobacteria	Gammaproteobacteria	Diplorickettsiales	Diplorickettsiaceae	Aquicella	NA
Seq329	Bacteria	Proteobacteria	Alphaproteobacteria	Sphingomonadales	Sphingomonadaceae	Sphingomonas	jaspsi
Seq332	Bacteria	Proteobacteria	Alphaproteobacteria	Rhodospirillales	Magnetospiraceae	NA	NA
Seq429	Bacteria	Proteobacteria	Alphaproteobacteria	Acetobacterales	Acetobacteraceae	NA	NA
Seq195	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	Pseudorhodoplanes	NA
Seq144	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	Pseudolabrys	NA
Seq369	Bacteria	Proteobacteria	Alphaproteobacteria	Micropepsales	Micropepsaceae	NA	NA
Seq362	Bacteria	Proteobacteria	Alphaproteobacteria	Sphingomonadales	Sphingomonadaceae	Altererythrobacter	NA
Seq428	Bacteria	Proteobacteria	Alphaproteobacteria	Caulobacterales	Caulobacteraceae	Phenylobacterium	NA
Seq347	Bacteria	Nitrospirota	Nitrospiria	Nitrospirales	Nitrospiraceae	Nitrospira	japonica
Seq328	Bacteria	Myxococcota	Polyangia	Haliangiales	Haliangiaceae	Haliangium	NA
Seq348	Bacteria	Myxococcota	Polyangia	Polyangiales	BIrii41	NA	NA
Seq308	Bacteria	Myxococcota	Polyangia	Polyangiales	BIrii41	NA	NA
Seq256	Bacteria	Myxococcota	Polyangia	Polyangiales	Polyangiaceae	Minicystis	NA
Seq302	Bacteria	Bdellovibrionota	Oligoflexia	0319-6G20	NA	NA	NA
Seq435	Bacteria	Actinobacteriota	Actinobacteria	Propionibacteriales	Propionibacteriaceae	Microlunatus	NA
Seq291	Bacteria	Actinobacteriota	Acidimicrobiia	IMCC26256	NA	NA	NA
Seq392	Bacteria	Actinobacteriota	Acidimicrobiia	Microtrichales	Ilumatobacteraceae	NA	NA
Seq321	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	NS11-12 marine group	NA	NA
Seq186	Bacteria	Firmicutes	Bacilli	Paenibacillales	Paenibacillaceae	Paenibacillus	NA
Seq204	Bacteria	Firmicutes	Bacilli	Bacillales	Planococcaceae	Paenisporosarcina	NA
Seq146	Bacteria	Spirochaetota	Spirochaetia	Spirochaetales	Spirochaetaceae	Spirochaeta 2	NA
Seq359	Bacteria	Patescibacteria	Saccharimonadia	Saccharimonadales	LWQ8	NA	NA
Seq269	Bacteria	Chloroflexi	Chloroflexia	Chloroflexales	Roseiflexaceae	NA	NA
Seq267	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Opitutales	Opitutaceae	Lacunisphaera	NA
Seq320	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Opitutales	Opitutaceae	Opitutus	NA
Seq203	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Chthoniobacterales	Terrimicrobiaceae	Terrimicrobium	NA
Seq497	Bacteria	Planctomycetota	Planctomycetes	Pirellulales	Pirellulaceae	NA	NA
Seq384	Bacteria	Planctomycetota	Planctomycetes	Planctomycetales	NA	NA	NA
Seq155	Bacteria	Acidobacteriota	Blastocatellia	Blastocatellales	Blastocatellaceae	NA	NA
Seq350	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	env.OPS 17	NA	NA
Seq224	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Terrimonas	NA
Seq409	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Edaphobaculum	NA
Seq337	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Taibaiella	NA
Seq494	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Amoebophilaceae	Candidatus Amoebophilus	NA
Seq277	Bacteria	Proteobacteria	Gammaproteobacteria	NA	NA	NA	NA
Seq191	Bacteria	Proteobacteria	Gammaproteobacteria	Gammaproteobacteria Incertae Sedis	Unknown Family	Acidibacter	NA
Seq335	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Xanthomonadaceae	Luteimonas	vadosa
Seq157	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Rhodanobacteraceae	Dokdonella	NA
Seq385	Bacteria	Proteobacteria	Gammaproteobacteria	Salinisphaerales	Solimonadaceae	NA	NA

$plum \(plum\)ps phyloseq-class experiment-level object otu_table() OTU Table: [ 30 taxa and 35 samples ] sample_data() Sample Data: [ 35 samples by 3 sample variables ] tax_table() Taxonomy Table: [ 30 taxa by 7 taxonomic ranks ] phy_tree() Phylogenetic Tree: [ 30 tips and 29 internal nodes ] refseq() DNAStringSet: [ 30 reference sequences ]

\(plum\)amp ampvis2 object with 5 elements. Summary of OTU table: Samples OTUs Total#Reads Min#Reads Max#Reads Median#Reads 35 30 17172 0 3034 103 Avg#Reads 490.63

Assigned taxonomy: Kingdom Phylum Class Order Family Genus Species 30(100%) 30(100%) 30(100%) 30(100%) 27(90%) 16(53.33%) 1(3.33%)

Metadata variables: 4 SampleID, Day, Description, Group

\(plum\)heat \(plum\)heat_rel \(plum\)tree \(plum\)taxa

	Kingdom	Phylum	Class	Order	Family	Genus	Species
Seq161	Bacteria	Proteobacteria	Gammaproteobacteria	Diplorickettsiales	Diplorickettsiaceae	Aquicella	NA
Seq181	Bacteria	Proteobacteria	Gammaproteobacteria	CCD24	NA	NA	NA
Seq207	Bacteria	Cyanobacteria	Vampirivibrionia	Obscuribacterales	Obscuribacteraceae	NA	NA
Seq219	Bacteria	Proteobacteria	Alphaproteobacteria	Sphingomonadales	Sphingomonadaceae	Sphingomonas	naasensis
Seq216	Bacteria	Proteobacteria	Alphaproteobacteria	Dongiales	Dongiaceae	Dongia	NA
Seq220	Bacteria	Proteobacteria	Alphaproteobacteria	Reyranellales	Reyranellaceae	Reyranella	NA
Seq90	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	Pseudolabrys	NA
Seq271	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	NA	NA
Seq111	Bacteria	Proteobacteria	Alphaproteobacteria	Sphingomonadales	Sphingomonadaceae	Sphingobium	NA
Seq33	Bacteria	Proteobacteria	Alphaproteobacteria	Caulobacterales	Caulobacteraceae	Caulobacter	NA
Seq189	Bacteria	Actinobacteriota	Thermoleophilia	Solirubrobacterales	Solirubrobacteraceae	Solirubrobacter	NA
Seq85	Bacteria	Myxococcota	Polyangia	Polyangiales	BIrii41	NA	NA
Seq208	Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Microbacteriaceae	Galbitalea	NA
Seq218	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	Sphingobacteriaceae	Mucilaginibacter	NA
Seq215	Bacteria	Firmicutes	Bacilli	Bacillales	Bacillaceae	Bacillus	NA
Seq177	Bacteria	Fibrobacterota	Fibrobacteria	Fibrobacterales	Fibrobacteraceae	NA	NA
Seq375	Bacteria	Chloroflexi	Chloroflexia	Thermomicrobiales	JG30-KF-CM45	NA	NA
Seq430	Bacteria	Planctomycetota	Phycisphaerae	Phycisphaerales	Phycisphaeraceae	NA	NA
Seq368	Bacteria	Planctomycetota	Planctomycetes	Pirellulales	Pirellulaceae	NA	NA
Seq152	Bacteria	Planctomycetota	Planctomycetes	Gemmatales	Gemmataceae	Gemmata	NA
Seq304	Bacteria	Planctomycetota	Planctomycetes	Planctomycetales	NA	NA	NA
Seq141	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Terrimonas	NA
Seq343	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	NA	NA
Seq412	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Edaphobaculum	NA
Seq457	Bacteria	Proteobacteria	Gammaproteobacteria	Coxiellales	Coxiellaceae	Coxiella	NA
Seq130	Bacteria	Proteobacteria	Gammaproteobacteria	R7C24	NA	NA	NA
Seq346	Bacteria	Proteobacteria	Gammaproteobacteria	Salinisphaerales	Solimonadaceae	Alkanibacter	NA
Seq474	Bacteria	Proteobacteria	Gammaproteobacteria	Diplorickettsiales	Diplorickettsiaceae	NA	NA
Seq472	Bacteria	Proteobacteria	Gammaproteobacteria	Diplorickettsiales	Diplorickettsiaceae	NA	NA
Seq227	Bacteria	Proteobacteria	Gammaproteobacteria	Diplorickettsiales	Diplorickettsiaceae	NA	NA

$red \(red\)ps phyloseq-class experiment-level object otu_table() OTU Table: [ 75 taxa and 35 samples ] sample_data() Sample Data: [ 35 samples by 3 sample variables ] tax_table() Taxonomy Table: [ 75 taxa by 7 taxonomic ranks ] phy_tree() Phylogenetic Tree: [ 75 tips and 74 internal nodes ] refseq() DNAStringSet: [ 75 reference sequences ]

\(red\)amp ampvis2 object with 5 elements. Summary of OTU table: Samples OTUs Total#Reads Min#Reads Max#Reads Median#Reads 35 75 66650 241 5339 1528 Avg#Reads 1904.29

Assigned taxonomy: Kingdom Phylum Class Order Family Genus Species 75(100%) 75(100%) 75(100%) 74(98.67%) 74(98.67%) 66(88%) 11(14.67%)

Metadata variables: 4 SampleID, Day, Description, Group

\(red\)heat \(red\)heat_rel \(red\)tree \(red\)taxa

	Kingdom	Phylum	Class	Order	Family	Genus	Species
Seq117	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Burkholderiaceae	Burkholderia	NA
Seq60	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Alcaligenaceae	NA	NA
Seq121	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	Variovorax	NA
Seq383	Bacteria	Proteobacteria	Alphaproteobacteria	Ferrovibrionales	Ferrovibrionaceae	Ferrovibrio	soli
Seq228	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	NA	NA
Seq178	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Beijerinckiaceae	Methylobacterium-Methylorubrum	NA
Seq171	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Beijerinckiaceae	Bosea	thiooxidans
Seq341	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Beijerinckiaceae	Bosea	NA
Seq89	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	Tardiphaga	robiniae
Seq31	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Xanthobacteraceae	Starkeya	NA
Seq160	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Hyphomicrobiaceae	Hyphomicrobium	NA
Seq251	Bacteria	Proteobacteria	Alphaproteobacteria	Sphingomonadales	Sphingomonadaceae	Sphingopyxis	NA
Seq373	Bacteria	Proteobacteria	Alphaproteobacteria	Sphingomonadales	Sphingomonadaceae	Altererythrobacter	NA
Seq108	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	Mesorhizobium	NA
Seq71	Bacteria	Actinobacteriota	Thermoleophilia	Solirubrobacterales	Solirubrobacteraceae	Conexibacter	NA
Seq338	Bacteria	Myxococcota	Polyangia	Polyangiales	Polyangiaceae	Pajaroellobacter	NA
Seq230	Bacteria	Myxococcota	Polyangia	Polyangiales	Polyangiaceae	Pajaroellobacter	NA
Seq88	Bacteria	Myxococcota	Polyangia	Polyangiales	Polyangiaceae	Sorangium	NA
Seq303	Bacteria	Actinobacteriota	Acidimicrobiia	Microtrichales	Ilumatobacteraceae	NA	NA
Seq93	Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Promicromonosporaceae	Promicromonospora	NA
Seq366	Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Microbacteriaceae	Leifsonia	NA
Seq134	Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Microbacteriaceae	NA	aoyamense
Seq411	Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Microbacteriaceae	Agromyces	NA
Seq115	Bacteria	Actinobacteriota	Actinobacteria	Corynebacteriales	Mycobacteriaceae	Mycobacterium	NA
Seq128	Bacteria	Actinobacteriota	Actinobacteria	Streptosporangiales	Streptosporangiaceae	Herbidospora	mongoliensis
Seq398	Bacteria	Actinobacteriota	Actinobacteria	Streptosporangiales	Streptosporangiaceae	Nonomuraea	NA
Seq78	Bacteria	Actinobacteriota	Actinobacteria	Streptosporangiales	Thermomonosporaceae	Actinocorallia	NA
Seq193	Bacteria	Actinobacteriota	Actinobacteria	Streptomycetales	Streptomycetaceae	Streptomyces	NA
Seq148	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	Sphingobacteriaceae	Pedobacter	NA
Seq307	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	NS11-12 marine group	NA	NA
Seq431	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	NS11-12 marine group	NA	NA
Seq205	Bacteria	Firmicutes	Bacilli	Paenibacillales	Paenibacillaceae	Paenibacillus	lautus
Seq86	Bacteria	Firmicutes	Bacilli	Paenibacillales	Paenibacillaceae	Paenibacillus	NA
Seq51	Bacteria	Firmicutes	Bacilli	Paenibacillales	Paenibacillaceae	Paenibacillus	NA
Seq18	Bacteria	Firmicutes	Bacilli	Paenibacillales	Paenibacillaceae	Paenibacillus	NA
Seq192	Bacteria	Firmicutes	Bacilli	Paenibacillales	Paenibacillaceae	Paenibacillus	NA
Seq266	Bacteria	Firmicutes	Bacilli	Paenibacillales	Paenibacillaceae	Paenibacillus	NA
Seq278	Bacteria	Firmicutes	Bacilli	Bacillales	Planococcaceae	Domibacillus	NA
Seq99	Bacteria	Firmicutes	Bacilli	Bacillales	Bacillaceae	Bacillus	NA
Seq53	Bacteria	Firmicutes	Bacilli	Bacillales	Planococcaceae	Lysinibacillus	NA
Seq118	Bacteria	Firmicutes	Bacilli	Bacillales	Planococcaceae	Lysinibacillus	NA
Seq76	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Chthoniobacterales	Terrimicrobiaceae	Terrimicrobium	NA
Seq232	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Chthoniobacterales	Terrimicrobiaceae	Terrimicrobium	NA
Seq168	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Chthoniobacterales	Terrimicrobiaceae	Terrimicrobium	NA
Seq387	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Chthoniobacterales	Chthoniobacteraceae	Chthoniobacter	NA
Seq285	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Chthoniobacterales	Chthoniobacteraceae	Chthoniobacter	NA
Seq217	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Verrucomicrobiales	Verrucomicrobiaceae	Verrucomicrobium	spinosum
Seq190	Bacteria	Planctomycetota	Planctomycetes	Gemmatales	Gemmataceae	Gemmata	NA
Seq249	Bacteria	Planctomycetota	Planctomycetes	Isosphaerales	Isosphaeraceae	Singulisphaera	NA
Seq254	Bacteria	Acidobacteriota	Acidobacteriae	Acidobacteriales	Acidobacteriaceae (Subgroup 1)	Edaphobacter	NA
Seq67	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq176	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq268	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Pseudoflavitalea	NA
Seq32	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Pseudoflavitalea	NA
Seq84	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Pseudoflavitalea	NA
Seq459	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Niastella	NA
Seq40	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Niastella	hibisci
Seq173	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Niastella	NA
Seq246	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Niastella	NA
Seq112	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Flavitalea	NA
Seq126	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	ginsengihumi
Seq102	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	arvensicola
Seq309	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	soli
Seq70	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq262	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq301	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Taibaiella	NA
Seq572	Bacteria	Proteobacteria	Gammaproteobacteria	Pseudomonadales	Moraxellaceae	NA	NA
Seq436	Bacteria	Proteobacteria	Gammaproteobacteria	Steroidobacterales	Steroidobacteraceae	Steroidobacter	NA
Seq261	Bacteria	Proteobacteria	Gammaproteobacteria	Gammaproteobacteria Incertae Sedis	Unknown Family	Acidibacter	NA
Seq298	Bacteria	Proteobacteria	Gammaproteobacteria	Gammaproteobacteria Incertae Sedis	Unknown Family	Acidibacter	NA
Seq354	Bacteria	Proteobacteria	Gammaproteobacteria	Gammaproteobacteria Incertae Sedis	Unknown Family	Acidibacter	NA
Seq226	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Rhodanobacteraceae	Tahibacter	NA
Seq147	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Xanthomonadaceae	Xanthomonas	NA
Seq370	Bacteria	Proteobacteria	Gammaproteobacteria	Diplorickettsiales	Diplorickettsiaceae	NA	NA
Seq486	Bacteria	Proteobacteria	Gammaproteobacteria	NA	NA	NA	NA

$salmon \(salmon\)ps phyloseq-class experiment-level object otu_table() OTU Table: [ 73 taxa and 35 samples ] sample_data() Sample Data: [ 35 samples by 3 sample variables ] tax_table() Taxonomy Table: [ 73 taxa by 7 taxonomic ranks ] phy_tree() Phylogenetic Tree: [ 73 tips and 72 internal nodes ] refseq() DNAStringSet: [ 73 reference sequences ]

\(salmon\)amp ampvis2 object with 5 elements. Summary of OTU table: Samples OTUs Total#Reads Min#Reads Max#Reads Median#Reads 35 73 115332 0 20534 779 Avg#Reads 3295.2

Assigned taxonomy: Kingdom Phylum Class Order Family Genus Species 73(100%) 73(100%) 73(100%) 72(98.63%) 71(97.26%) 66(90.41%) 12(16.44%)

Metadata variables: 4 SampleID, Day, Description, Group

\(salmon\)heat \(salmon\)heat_rel \(salmon\)tree \(salmon\)taxa

	Kingdom	Phylum	Class	Order	Family	Genus	Species
Seq79	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Oxalobacteraceae	Massilia	armeniaca
Seq122	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Oxalobacteraceae	NA	NA
Seq179	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Oxalobacteraceae	Pseudoduganella	NA
Seq145	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Oxalobacteraceae	Pseudoduganella	eburnea
Seq154	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Oxalobacteraceae	Massilia	NA
Seq222	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Oxalobacteraceae	Massilia	NA
Seq159	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Burkholderiaceae	Cupriavidus	NA
Seq8	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Burkholderiaceae	Cupriavidus	NA
Seq14	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Alcaligenaceae	Achromobacter	NA
Seq54	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	NA	paradoxus
Seq95	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	Xylophilus	NA
Seq340	Bacteria	Proteobacteria	Gammaproteobacteria	Burkholderiales	Comamonadaceae	Rhizobacter	NA
Seq264	Bacteria	Proteobacteria	Alphaproteobacteria	Sphingomonadales	Sphingomonadaceae	Sphingomonas	mucosissima
Seq333	Bacteria	Proteobacteria	Alphaproteobacteria	Reyranellales	Reyranellaceae	Reyranella	NA
Seq158	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Beijerinckiaceae	Microvirga	NA
Seq248	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	Allorhizobium	NA
Seq245	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Devosiaceae	Devosia	neptuniae
Seq185	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	Shinella	NA
Seq156	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	NA	NA
Seq42	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	NA	NA
Seq286	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	Ensifer	NA
Seq21	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	Allorhizobium	NA
Seq107	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	Neorhizobium	NA
Seq231	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	Allorhizobium	azooxidifex
Seq140	Bacteria	Proteobacteria	Alphaproteobacteria	Rhizobiales	Rhizobiaceae	NA	NA
Seq378	Bacteria	Proteobacteria	Alphaproteobacteria	Caulobacterales	Caulobacteraceae	Phenylobacterium	mobile
Seq374	Bacteria	Myxococcota	Polyangia	Haliangiales	Haliangiaceae	Haliangium	NA
Seq257	Bacteria	Myxococcota	Polyangia	Polyangiales	Polyangiaceae	Pajaroellobacter	NA
Seq223	Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Promicromonosporaceae	Cellulosimicrobium	NA
Seq83	Bacteria	Actinobacteriota	Actinobacteria	Micrococcales	Microbacteriaceae	Microbacterium	NA
Seq68	Bacteria	Actinobacteriota	Actinobacteria	Corynebacteriales	Mycobacteriaceae	Mycobacterium	NA
Seq293	Bacteria	Actinobacteriota	Actinobacteria	Glycomycetales	Glycomycetaceae	Glycomyces	NA
Seq72	Bacteria	Actinobacteriota	Actinobacteria	Streptomycetales	Streptomycetaceae	Streptomyces	NA
Seq101	Bacteria	Actinobacteriota	Actinobacteria	Streptomycetales	Streptomycetaceae	Streptomyces	NA
Seq265	Bacteria	Actinobacteriota	Actinobacteria	Streptomycetales	Streptomycetaceae	Streptomyces	NA
Seq260	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	NA	NA	NA
Seq180	Bacteria	Bacteroidota	Bacteroidia	Sphingobacteriales	Sphingobacteriaceae	Pedobacter	panaciterrae
Seq142	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Verrucomicrobiales	Verrucomicrobiaceae	Roseimicrobium	NA
Seq163	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq253	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq116	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq184	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Microscillaceae	Ohtaekwangia	NA
Seq52	Bacteria	Bacteroidota	Bacteroidia	Flavobacteriales	Flavobacteriaceae	Flavobacterium	NA
Seq187	Bacteria	Bacteroidota	Bacteroidia	Flavobacteriales	Weeksellaceae	Chryseobacterium	ginsenosidimutans
Seq172	Bacteria	Bacteroidota	Bacteroidia	Flavobacteriales	Weeksellaceae	Chryseobacterium	NA
Seq283	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Flavitalea	NA
Seq113	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Niastella	NA
Seq110	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Niastella	NA
Seq132	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Pseudoflavitalea	NA
Seq37	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq162	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq182	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	humicola
Seq92	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq80	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	pinensis
Seq2	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq221	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq20	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq282	Bacteria	Bacteroidota	Bacteroidia	Chitinophagales	Chitinophagaceae	Chitinophaga	NA
Seq38	Bacteria	Bacteroidota	Bacteroidia	Cytophagales	Spirosomaceae	Dyadobacter	NA
Seq237	Bacteria	Proteobacteria	Gammaproteobacteria	Steroidobacterales	Steroidobacteraceae	Steroidobacter	agariperforans
Seq234	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Xanthomonadaceae	Pseudoxanthomonas	NA
Seq59	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Xanthomonadaceae	Stenotrophomonas	NA
Seq105	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Xanthomonadaceae	Stenotrophomonas	NA
Seq344	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Xanthomonadaceae	Lysobacter	NA
Seq41	Bacteria	Proteobacteria	Gammaproteobacteria	Xanthomonadales	Xanthomonadaceae	Lysobacter	NA
Seq243	Bacteria	Proteobacteria	Gammaproteobacteria	NA	NA	NA	NA
Seq406	Bacteria	Proteobacteria	Gammaproteobacteria	Legionellales	Legionellaceae	Legionella	NA
Seq139	Bacteria	Proteobacteria	Gammaproteobacteria	Pseudomonadales	Pseudomonadaceae	Pseudomonas	NA
Seq24	Bacteria	Proteobacteria	Gammaproteobacteria	Pseudomonadales	Pseudomonadaceae	Pseudomonas	NA
Seq27	Bacteria	Proteobacteria	Gammaproteobacteria	Pseudomonadales	Pseudomonadaceae	Pseudomonas	NA
Seq87	Bacteria	Proteobacteria	Gammaproteobacteria	Pseudomonadales	Pseudomonadaceae	Pseudomonas	NA
Seq55	Bacteria	Proteobacteria	Gammaproteobacteria	Pseudomonadales	Pseudomonadaceae	Pseudomonas	NA
Seq201	Bacteria	Proteobacteria	Gammaproteobacteria	Enterobacterales	Enterobacteriaceae	Klebsiella	NA

mpd по стадиям

physeq_merged <- merge_samples(ps.f, "Group", fun=sum)

## Found more than one class "phylo" in cache; using the first, from namespace 'phyloseq'

## Also defined by 'tidytree'

## Found more than one class "phylo" in cache; using the first, from namespace 'phyloseq'

## Also defined by 'tidytree'

## Found more than one class "phylo" in cache; using the first, from namespace 'phyloseq'

## Also defined by 'tidytree'

## Found more than one class "phylo" in cache; using the first, from namespace 'phyloseq'

## Also defined by 'tidytree'

ps.f@sam_data

##                 Day Description  Group
## straw-16s-D01-1 D01       D01_1  early
## straw-16s-D01-2 D01       D01_2  early
## straw-16s-D01-3 D01       D01_3  early
## straw-16s-D03-1 D03       D03_1  early
## straw-16s-D03-2 D03       D03_2  early
## straw-16s-D03-3 D03       D03_3  early
## straw-16s-D05-1 D05       D05_1  early
## straw-16s-D05-2 D05       D05_2  early
## straw-16s-D05-3 D05       D05_3  early
## straw-16s-D05-4 D05       D05_4  early
## straw-16s-D05-5 D05       D05_5  early
## straw-16s-D07-2 D07       D07_2 middle
## straw-16s-D07-3 D07       D07_3 middle
## straw-16s-D08-1 D08       D08_1 middle
## straw-16s-D08-2 D08       D08_2 middle
## straw-16s-D08-3 D08       D08_3 middle
## straw-16s-D10-1 D10       D10_1 middle
## straw-16s-D10-2 D10       D10_2 middle
## straw-16s-D10-3 D10       D10_3 middle
## straw-16s-D10-4 D10       D10_4 middle
## straw-16s-D10-5 D10       D10_5 middle
## straw-16s-D12-1 D12       D12_1   late
## straw-16s-D12-2 D12       D12_2   late
## straw-16s-D12-3 D12       D12_3   late
## straw-16s-D13-1 D13       D13_1   late
## straw-16s-D13-2 D13       D13_2   late
## straw-16s-D13-3 D13       D13_3   late
## straw-16s-D14-1 D14       D14_1   late
## straw-16s-D14-2 D14       D14_2   late
## straw-16s-D14-3 D14       D14_3   late
## straw-16s-D14-4 D14       D14_4   late
## straw-16s-D14-5 D14       D14_5   late
## straw-16s-D15-1 D15       D15_1   late
## straw-16s-D15-2 D15       D15_2   late
## straw-16s-D15-3 D15       D15_3   late

# picante::mpd(l_vst$blue$ps@otu_table@.Data, cophenetic(l_vst$blue$ps@phy_tree)) %>% 
#   mean(na.rm = TRUE)
# 
# picante::mpd(l_vst$salmon$ps@otu_table@.Data, cophenetic(l_vst$salmon$ps@phy_tree)) %>% 
#   mean(na.rm = TRUE)

picante::ses.mpd(physeq_merged@otu_table@.Data, cophenetic(physeq_merged@phy_tree)) 

##        ntaxa  mpd.obs mpd.rand.mean mpd.rand.sd mpd.obs.rank  mpd.obs.z
## early    364 1.742096      1.862383  0.02930118            1 -4.1051776
## middle   490 1.839665      1.863938  0.02298162          147 -1.0561720
## late     822 1.877129      1.863599  0.01354403          842  0.9989395
##        mpd.obs.p runs
## early      0.001  999
## middle     0.147  999
## late       0.842  999

0.7 Add a metadata

list.files("meta/")

## [1] "cell_realtime_stat.xlsx" "cell_resp_ch_stat.xlsx" 
## [3] "period_legend.xlsx"

period_legend - соответствие номеров мешочков в 16S с днями, думаю лучше заменить везде обозначения 1, 3 и тд на дни cell_resp - данные по дыханию, по ним какую-нибудь простую статистику. Да, повторностей для эксперимента и контроля разное количество cell_realtime - это циклы выхода целлюлаз по реалтайму, я их нормировала по 16S, есть в этой же таблице. Что с ними делать особо не знаю, вроде вообще решили выкинуть

realtime.data <- readxl::read_excel("meta/cell_realtime_stat.xlsx")
period.data <- readxl::read_excel("meta/period_legend.xlsx")
resp.data <- readxl::read_excel("meta/cell_resp_ch_stat.xlsx")
realtime.data

## # A tibble: 36 × 29
##    id        day contr…¹ CELL_…² CELL_…³ CELL_…⁴ CELL_…⁵ CELL_…⁶ CELL_…⁷ CELL_…⁸
##    <chr>   <dbl>   <dbl>   <dbl>   <dbl>   <dbl>   <dbl>   <dbl>   <dbl>   <dbl>
##  1 G01-1-1     3    15.6    33.2    31.1    33.6    36.6    33.8    38.0    33.0
##  2 G01-1-2     3    15.5    33.3    31.3    32.9    36.2    33.8    41      32.7
##  3 G01-1-3     3    15.5    33.6    31.2    32.9    35.8    33.3    38.2    32.9
##  4 G01-2-1     3    17.1    36.0    31.1    33.1    36.9    36.4    38.8    33.8
##  5 G01-2-2     3    17.3    35.6    31.0    32.8    37.8    35.7    40.1    33.6
##  6 G01-2-3     3    17.1    36      31.1    32.8    38.1    35.2    37.6    33.6
##  7 G01-3-1     3    16.4    35.4    31.6    34.9    39.1    35.3    40.9    33.2
##  8 G01-3-2     3    16.5    35.6    31.3    34.4    37.9    34.6    38.9    33.1
##  9 G01-3-3     3    16.4    35.8    31.5    34.4    41.6    35.0    36.5    33.4
## 10 G05-1-1    28    18.2    26.3    29.1    29.4    28.2    28.4    37.4    34.5
## # … with 26 more rows, 19 more variables: CELL_193122 <dbl>, CELL_73229 <dbl>,
## #   CELL_47814 <dbl>, CELL_163125 <dbl>, CELL_73266 <dbl>, CELL_88582 <dbl>,
## #   CELL_63583 <dbl>, CELL_14199 <dbl>, CELL_95616 <dbl>, CELL_63504 <dbl>,
## #   CELL_08643 <chr>, CELL_199599 <dbl>, CELL_01426 <dbl>, CELL_71601 <dbl>,
## #   CELL_45099 <dbl>, CELL_191900 <dbl>, CELL_99463 <dbl>, CELL_74579 <dbl>,
## #   CELL_183403 <dbl>, and abbreviated variable names ¹​contr_16S, ²​CELL_172283,
## #   ³​CELL_203163, ⁴​CELL_83325, ⁵​CELL_188413, ⁶​CELL_109631, ⁷​CELL_188119, …

0.7.1 Realtime data

impute.mean <- function(x) replace(x, is.na(x), mean(x, na.rm = TRUE))

realtime_data <- realtime.data %>% 
  mutate(CELL_08643 = as.numeric(CELL_08643)) %>% 
  group_by(day) %>%
  mutate(nice_cell = impute.mean(CELL_08643)) %>% 
  mutate(day = as.factor(day))

## Warning in mask$eval_all_mutate(quo): NAs introduced by coercion

realtime_zjena <- readxl::read_excel("cellulases_gene_expression(1).xlsx")
geom_mean <-function(x){exp(mean(log(x)))}

realtime_zjena_geom <- realtime_zjena %>% 
  mutate(repeats = paste0(realtime_zjena$week, "-", rep(1:3, 3, each=3))) %>% 
  relocate(repeats, 1) %>% 
  group_by(repeats) %>% 
  summarise_if(is.numeric, geom_mean) %>% 
  mutate(week = as.factor(week)) %>% 
  arrange(week)

UPGMA целлюлаз(дистанция - брей).
Не очень понимаю, что это значит. В зависимости от дистанции получаются совсем разные кластеризации.

realtime_matrix <- realtime_zjena_geom %>% 
  column_to_rownames("repeats") %>% 
  select_if(is.numeric) %>% 
  as.matrix()

hcl <- hclust(vegan::vegdist(t(realtime_matrix), method="bray"), "average")
plot(hcl)

А вот - евклидова дистанция.
Ничего общего с предыдущей картинкой

hcl <- hclust(vegan::vegdist(t(realtime_matrix), method="euclidian"), "average")
plot(hcl)

Корплот целлюлаз.
Только корреляции

m = cor(realtime_matrix)
corrplot::corrplot(m)

Только достоверные корреляции
В общем - кластеры есть - но корреляцие недостоверные(за некоторыми исключениями)

cor_test_mat <- psych::corr.test(realtime_matrix)$p
corrplot::corrplot(m, p.mat = cor_test_mat, method = 'circle', type = 'lower', insig='blank',
         order = 'AOE', diag = FALSE)$corrPos -> p1

# text(p1$x, p1$y, round(p1$corr, 2))

То же, но матрица уже прологарифмирована.

cor_test_mat <- psych::corr.test(log(realtime_matrix))
corrplot::corrplot(cor_test_mat$r, p.mat = cor_test_mat$p, method = 'circle', type = 'lower', insig='blank',
         order = 'AOE', diag = FALSE)$corrPos -> p1

# text(p1$x, p1$y, round(p1$corr, 2))

add phylogenic tree (mafft - iqtree(ML))

realtime_tree <- ape::read.tree("al_chz.fasta.contree")
plot(realtime_tree)

library(tidyverse)

realtime_data %>% 
  select(c("day", "id", "contr_16S", "CELL_172283")) %>% 
  mutate(bio_repl = gsub("-[1-3]$", "", id)) %>% 
  group_by(bio_repl, day) %>% 
  summarise(contr_tmean = mean(contr_16S),
            data_tmean = mean(CELL_172283)) %>% 
  mutate(dCt = data_tmean - contr_tmean)

## `summarise()` has grouped output by 'bio_repl'. You can override using the
## `.groups` argument.

## # A tibble: 12 × 5
## # Groups:   bio_repl [12]
##    bio_repl day   contr_tmean data_tmean   dCt
##    <chr>    <fct>       <dbl>      <dbl> <dbl>
##  1 G01-1    3            15.5       33.4 17.8 
##  2 G01-2    3            17.2       35.9 18.7 
##  3 G01-3    3            16.4       35.6 19.2 
##  4 G05-1    28           18.4       26.2  7.81
##  5 G05-2    28           16.5       26.2  9.73
##  6 G05-3    28           18.5       32.0 13.6 
##  7 G10-1    91           17.4       26.2  8.72
##  8 G10-2    91           16.3       24.1  7.79
##  9 G10-3    91           17.5       26.3  8.81
## 10 G14-1    161          18.0       28.1 10.1 
## 11 G14-2    161          16.5       27.0 10.6 
## 12 G14-3    161          16.9       25.4  8.41

resp_data <- resp.data %>% 
  group_by(day) %>%
  mutate(control = impute.mean(control)) %>% 
  mutate(straw = impute.mean(straw)) 

resp_data

## # A tibble: 164 × 3
## # Groups:   day [27]
##      day control straw
##    <dbl>   <dbl> <dbl>
##  1     0    250   250 
##  2     3    285.  723.
##  3     3    263.  832.
##  4     3    131.  657.
##  5     3    307.  525.
##  6     3    241.  744.
##  7     3    245.  701.
##  8     3    245.  657.
##  9     7    274.  690.
## 10     7    296.  690.
## # … with 154 more rows

0.7.2 Knee plot

Дыхание - median(straw)/median(control)

Mожно использовать сторонний пакет(KneeArrower) что понять в каком месте knee_plot происходит перелом. Для элиминации повторностей возьмем медиану.   Я хз как этот пакет работает(ищет производную, но как сглаживает - хз, там матан), но он говорит что перелом происходит скорее на 60-80 днях.

(я исправил ошибки - стало ближе к твоим данным)

https://github.com/agentlans/KneeArrower - вот здесь можно почитать про матан

# resp_data %>% 
#   filter(!day == 0) %>% 
#   group_by(day) %>% 
#   summarise(median_control = median(control),
#             median_straw = median(straw)) %>% 
#   mutate(rel = median_straw/median_control) %>% 
#   ggplot() +
#   geom_point(aes(x = day, y = rel))

resp_median <- resp_data %>% 
  filter(!day == 0) %>% 
  group_by(day) %>% 
  summarise(median_control = median(control),
            median_straw = median(straw)) %>% 
  mutate(rel = median_straw/median_control)


thresholds <- c(0.25, 0.5, 0.75, 1)

# Find cutoff points at each threshold
cutoff.points <- lapply(thresholds, function(i) {
  findCutoff(resp_median$day, resp_median$rel, method="first", i)
})

x.coord <- sapply(cutoff.points, function(p) p$x)
y.coord <- sapply(cutoff.points, function(p) p$y)

# Plot the cutoff points on the scatterplot
plot(resp_median$day, resp_median$rel, pch=20, col="gray")
points(x.coord, y.coord, col="red", pch=20)
text(x.coord, y.coord, labels=thresholds, pos=4, col="red")

period_data <- period.data %>%
  mutate(bag_id = as.factor(bag_id))

period_data

## # A tibble: 15 × 2
##    bag_id   day
##    <fct>  <dbl>
##  1 1          3
##  2 2          7
##  3 3         14
##  4 4         21
##  5 5         28
##  6 6         35
##  7 7         49
##  8 8         63
##  9 9         77
## 10 10        91
## 11 11       105
## 12 12       119
## 13 13       140
## 14 14       161
## 15 15       182

0.7.3 альфа/дыхание

Ну я вот не очень понимаю что делать дальше - привязать кластеры к этой картинке?

resp_median_bags <- resp_median %>% 
  left_join(period.data, by="day") %>% 
  mutate(bag_id = as.factor(bag_id))

ps.f.r <- rarefy_even_depth(ps.f)

estimate_richness(ps.f.r) %>% 
  rownames_to_column("ID") %>% 
  mutate(bag_id = as.factor(
    as.numeric(
      gsub("\\..+","",
           gsub("straw\\.16s\\.D","", ID)
           )
      )
    )
    ) %>% 
  group_by(bag_id) %>% 
  summarise(Observed = mean(Observed),
            Shannon = mean(Shannon),
            InvSimpson = mean(InvSimpson)) %>% 
  left_join(period_data, by="bag_id") %>% 
  left_join(resp_median_bags, by="bag_id") %>% 
  mutate(
    Observed_scaled = scale(Observed),
    Shannon_scaled = scale(Shannon),
    InvSimpson_scaled = scale(InvSimpson),
    Respiration_scaled = scale(rel)
  ) %>% 
  select(c(bag_id, Observed_scaled, Shannon_scaled, InvSimpson_scaled, Respiration_scaled)) %>% 
  pivot_longer(c("Observed_scaled", "Shannon_scaled", "InvSimpson_scaled", "Respiration_scaled")) %>% 
  ggplot(aes(y = value, x = bag_id, group = name)) +
  geom_line(aes(color = name),
            alpha = 0.8) +
  theme_bw()

Корреляция -ртрицательная слабенькая, не особо достоверная и только Пирсона(которая работает для нормального распределения(у нас хроносерия, надо спирмана по хорошему))

alpha_resp <- phyloseq::estimate_richness(ps.f.r) %>% 
  rownames_to_column("ID") %>% 
  mutate(bag_id = as.factor(
    as.numeric(
      gsub("\\..+","",
           gsub("straw\\.16s\\.D","", ID)
           )
      )
    )
    ) %>% 
  group_by(bag_id) %>% 
  summarise(Observed = mean(Observed),
            Shannon = mean(Shannon),
            InvSimpson = mean(InvSimpson)) %>% 
  left_join(period_data, by="bag_id") %>% 
  left_join(resp_median_bags, by="bag_id") %>% 
  mutate(
    Observed_scaled = scale(Observed),
    Shannon_scaled = scale(Shannon),
    InvSimpson_scaled = scale(InvSimpson),
    Respiration_scaled = scale(rel)
  ) %>% 
  select(c(bag_id, Observed_scaled, Shannon_scaled, InvSimpson_scaled, Respiration_scaled))

cor.test(alpha_resp$Observed_scaled, alpha_resp$Respiration_scaled, method = "spearman")

## 
##  Spearman's rank correlation rho
## 
## data:  alpha_resp$Observed_scaled and alpha_resp$Respiration_scaled
## S = 230, p-value = 0.2629
## alternative hypothesis: true rho is not equal to 0
## sample estimates:
##        rho 
## -0.3939394

cor.test(alpha_resp$Shannon_scaled, alpha_resp$Respiration_scaled, method = "spearman")

## 
##  Spearman's rank correlation rho
## 
## data:  alpha_resp$Shannon_scaled and alpha_resp$Respiration_scaled
## S = 232, p-value = 0.2474
## alternative hypothesis: true rho is not equal to 0
## sample estimates:
##        rho 
## -0.4060606

cor.test(alpha_resp$InvSimpson_scaled, alpha_resp$Respiration_scaled, method = "spearman")

## 
##  Spearman's rank correlation rho
## 
## data:  alpha_resp$InvSimpson_scaled and alpha_resp$Respiration_scaled
## S = 226, p-value = 0.2956
## alternative hypothesis: true rho is not equal to 0
## sample estimates:
##       rho 
## -0.369697

cor.test(alpha_resp$Observed_scaled, alpha_resp$Respiration_scaled, method = "pearson")

## 
##  Pearson's product-moment correlation
## 
## data:  alpha_resp$Observed_scaled and alpha_resp$Respiration_scaled
## t = -2.7571, df = 8, p-value = 0.02479
## alternative hypothesis: true correlation is not equal to 0
## 95 percent confidence interval:
##  -0.9223009 -0.1220128
## sample estimates:
##        cor 
## -0.6980158

cor.test(alpha_resp$Shannon_scaled, alpha_resp$Respiration_scaled, method = "pearson")

## 
##  Pearson's product-moment correlation
## 
## data:  alpha_resp$Shannon_scaled and alpha_resp$Respiration_scaled
## t = -2.8453, df = 8, p-value = 0.02164
## alternative hypothesis: true correlation is not equal to 0
## 95 percent confidence interval:
##  -0.9255437 -0.1437787
## sample estimates:
##        cor 
## -0.7092031

cor.test(alpha_resp$InvSimpson_scaled, alpha_resp$Respiration_scaled, method = "pearson")

## 
##  Pearson's product-moment correlation
## 
## data:  alpha_resp$InvSimpson_scaled and alpha_resp$Respiration_scaled
## t = -2.2565, df = 8, p-value = 0.05401
## alternative hypothesis: true correlation is not equal to 0
## 95 percent confidence interval:
##  -0.899910986  0.009841998
## sample estimates:
##        cor 
## -0.6236489

0.8 Export all phyloseq object in one table

В табличке есть колонка cluster - “exl” в ней обозначает что это та часть датасета которая не пошла в WGCNA - мажоры в единичных семплах.

ps.m <- phyloseq::psmelt(ps.f)

ps.m <- ps.m %>% 
  mutate_if(is.character, as.factor) 

ps.data.out <- ps.m %>%
  select(-Group) %>% 
  pivot_wider(names_from = c(Day, Description, Sample), values_from = Abundance, values_fill = 0) 

#create empty dataframe with columnnames
external_empty_dataframe <- data.frame(OTU=factor(), cluster=factor(), stringsAsFactors = FALSE)

for (i in names(l_vst)) {
  a <- taxa_names(l_vst[[i]][["ps"]])
  b <- rep(i, length(a))
  d <- data.frame(OTU = as.factor(a),
                  cluster = as.factor(b))
  external_empty_dataframe <- rbind(external_empty_dataframe, d)
}

clusters.otu.df <- external_empty_dataframe
# add exl taxa -- taxa "exl"
d <- data.frame(OTU = as.factor(ps.exl.taxa),
                cluster = as.factor(rep("exl", length(ps.exl.taxa))))

clusters.otu.df <- rbind(clusters.otu.df, d)
ps.data.out.exl <- left_join(clusters.otu.df, ps.data.out, by="OTU")
# write.table(ps.data.out.exl, file = "ps.data.out.tsv", sep = "\t")
ps.data.out.exl

0.8.1 Несколько картинок по динамике предсталенности отдельных фил

Bdellovibrionota - хищники, индикатор развитого сообщества

ps.m %>% 
  filter(Phylum == "Bdellovibrionota") %>% 
  group_by(Description, Day) %>% 
  summarise(Bs = sum(Abundance)) %>% 
  ggplot() +
  geom_boxplot(aes(x = Day, y = Bs)) +
  theme_bw()

## `summarise()` has grouped output by 'Description'. You can override using the
## `.groups` argument.

Myxococcota - вроде бы тоже, но как нам известно могут быть целлулотитиками

ps.m %>% 
  filter(Phylum == "Myxococcota") %>% 
  group_by(Description, Day) %>% 
  summarise(Bs = sum(Abundance)) %>% 
  ggplot() +
  geom_boxplot(aes(x = Day, y = Bs)) +
  theme_bw()

## `summarise()` has grouped output by 'Description'. You can override using the
## `.groups` argument.

Археи появляются тоже на поздних стадиях - вообще я бы хотел бы опять развить тему важности азотного метаболизма на поздних стадиях разложения.
Оч хочется метагеном, но не этот.
Кроме того хочется отметить, что эти минорные группы возникают на D12

ps.m %>% 
  filter(Phylum == "Crenarchaeota") %>% 
  group_by(Description, Day) %>% 
  summarise(Bs = sum(Abundance)) %>% 
  ggplot() +
  geom_boxplot(aes(x = Day, y = Bs)) +
  theme_bw()

## `summarise()` has grouped output by 'Description'. You can override using the
## `.groups` argument.

Gammaproteobacteria - они треть кластера blue

ps.m %>% 
  filter(Class == "Gammaproteobacteria") %>% 
  group_by(Description, Day) %>% 
  summarise(Bs = sum(Abundance)) %>% 
  ggplot() +
  geom_boxplot(aes(x = Day, y = Bs)) +
  theme_bw()

## `summarise()` has grouped output by 'Description'. You can override using the
## `.groups` argument.

0.8.2 Боксплоты по филам

Все филы - представлененось логорифмирована по основанию 2
Отдельные точки - суммы абсолютных значений ридов по дням
Логорифмированы уже суммы, а не отдельные филотипы

#select only major phylums
top_phylum <- ps.m %>% 
  count(Phylum) %>% 
  arrange(desc(n)) %>% 
  top_n(10) %>% 
  pull(Phylum)

## Selecting by n

ps.m %>% 
  filter(Phylum %in% top_phylum) %>% 
  mutate(
    Phylum = as.character(Phylum),
    Class = as.character(Class),
    phylum = ifelse(Phylum == "Proteobacteria", Class, Phylum)
  ) %>% 
  group_by(Description, Day, phylum) %>% 
  filter(!is.na(phylum)) %>% 
  summarise(Bs = log2(sum(Abundance))) %>% 
  ggplot(aes(x = Day, y = Bs)) +
  geom_boxplot(fill="#4DBBD5B2", alpha=0.4) +
  theme_bw() +
  facet_wrap(~ phylum)

## `summarise()` has grouped output by 'Description', 'Day'. You can override
## using the `.groups` argument.

## Warning: Removed 46 rows containing non-finite values (stat_boxplot).

0.9 Добавим грибов

library(DESeq2)

ps_its <- readRDS("../../its/d2/ps_its")

amp_its <- phyloseq_to_ampvis2(ps_its)

ps.its.inall <- phyloseq::filter_taxa(ps_its, function(x) sum(x > 10) > (0.1*length(x)), TRUE)
ps.its.inall <- prune_taxa(taxa_sums(ps.its.inall) > 0, ps.its.inall)
Biostrings::writeXStringSet(ps.its.inall@refseq, file="ref_inall.fasta")
Biostrings::writeXStringSet(ps_its@refseq, file="ref.fasta")

tree <- ape::read.tree("../../its/d2/al.fasta.contree")

ps.its.inall@phy_tree <- tree
ps.its.inall  <- prune_taxa(taxa_sums(ps.its.inall) > 0, ps.its.inall)


ps.its.exl  <- phyloseq::filter_taxa(ps_its, function(x) sum(x > 10) < (0.1*length(x)), TRUE)
ps.its.exl  <- prune_taxa(taxa_sums(ps.its.exl) > 100, ps.its.exl)
ps.its.exl.taxa <- taxa_names(ps.its.exl)

ps.its.exl.taxa

##   [1] "Seq14"  "Seq34"  "Seq35"  "Seq38"  "Seq51"  "Seq55"  "Seq56"  "Seq57" 
##   [9] "Seq58"  "Seq60"  "Seq61"  "Seq64"  "Seq71"  "Seq72"  "Seq77"  "Seq80" 
##  [17] "Seq81"  "Seq82"  "Seq83"  "Seq84"  "Seq87"  "Seq88"  "Seq90"  "Seq95" 
##  [25] "Seq97"  "Seq98"  "Seq105" "Seq106" "Seq107" "Seq109" "Seq110" "Seq111"
##  [33] "Seq112" "Seq113" "Seq115" "Seq116" "Seq117" "Seq119" "Seq120" "Seq126"
##  [41] "Seq127" "Seq128" "Seq129" "Seq130" "Seq131" "Seq133" "Seq134" "Seq135"
##  [49] "Seq139" "Seq141" "Seq142" "Seq143" "Seq144" "Seq145" "Seq146" "Seq148"
##  [57] "Seq149" "Seq151" "Seq153" "Seq154" "Seq156" "Seq158" "Seq159" "Seq160"
##  [65] "Seq162" "Seq163" "Seq164" "Seq165" "Seq167" "Seq168" "Seq169" "Seq170"
##  [73] "Seq172" "Seq173" "Seq174" "Seq176" "Seq177" "Seq179" "Seq182" "Seq183"
##  [81] "Seq185" "Seq186" "Seq187" "Seq188" "Seq189" "Seq192" "Seq194" "Seq196"
##  [89] "Seq197" "Seq198" "Seq199" "Seq200" "Seq201" "Seq202" "Seq203" "Seq204"
##  [97] "Seq206" "Seq207" "Seq208" "Seq209" "Seq210" "Seq211" "Seq212" "Seq213"
## [105] "Seq214" "Seq216" "Seq217" "Seq218" "Seq219" "Seq220" "Seq221" "Seq222"
## [113] "Seq223" "Seq224" "Seq225" "Seq227" "Seq228" "Seq229" "Seq230" "Seq232"
## [121] "Seq233" "Seq234" "Seq236" "Seq237" "Seq238" "Seq239" "Seq240" "Seq241"
## [129] "Seq242" "Seq243" "Seq244" "Seq246" "Seq247" "Seq248" "Seq249" "Seq250"
## [137] "Seq251" "Seq252" "Seq253" "Seq255"

amp_its_inall <- phyloseq_to_ampvis2(ps.its.inall)

as.data.frame(ps_its@otu_table)

##                   Seq1 Seq2 Seq3 Seq4 Seq5 Seq6 Seq7 Seq8 Seq9 Seq10 Seq11
## straw-its2-D01-1     0    0    0    0    0 1297    0    0  831     0     0
## straw-its2-D01-2     0    0    0    0    0    0    0    0    0     0     0
## straw-its2-D01-3     0  883   27    0  630    0    0    0    0     0 11852
## straw-its2-D03-1     0    0 4212    0    0 1110    0  536    0     0     0
## straw-its2-D03-2     0    0 4432    0    0 1563    0 1697 2263     0     0
## straw-its2-D03-3     0    0 2453    0    0 2617    0 1245 2108     0     0
## straw-its2-D05-1     0    0 1188    0    0  117 2125  460    0     0     0
## straw-its2-D05-2     0    0 5819    0    0  721    0 1012    0     0     0
## straw-its2-D05-3     0    0 5416    0    0 2422    0  973 1298     0     0
## straw-its2-D05-4     0    0 5437    0    0  137 1415  960  298   365     0
## straw-its2-D05-5     0    0    0    0    0  881 1649  870 1009     0     0
## straw-its2-D07-1     0  122 2876 4674    0  788 5718 1666    0   113     0
## straw-its2-D07-2   920 5516 4192 4672 3760  660  546 2152 3012   428     0
## straw-its2-D07-3     0    0 3577  777    0  238 6781  559  674  1748   355
## straw-its2-D08-1    80    0  443  180  327  325 3435  915  804  3082     0
## straw-its2-D08-2  2024 3791   88 2126 2456  254    0    0    0     0     0
## straw-its2-D08-3     0    0 4113 7384    0   65  101 1972  690     0     0
## straw-its2-D10-1  3264    0 1825    0    0  585    0    0  579     0     0
## straw-its2-D10-2  6205  337 3298  574    0  848    0  596  949   164   180
## straw-its2-D10-3  2838    0  106 8478   29   74    0  182  193     0   145
## straw-its2-D10-4 10397 1098  672  279  792  476    0  372    0   404     0
## straw-its2-D10-5  7398    0    0  264    0    0    0    0 2219     0     0
## straw-its2-D12-1   208 5807    0 1756 3853  800    0  408  228   278     0
## straw-its2-D12-2  6419 5644  447   98 3533  210    0  744    0  1177   266
## straw-its2-D12-3  4376 1430  540 4655  908  686    0 1214    0   307   488
## straw-its2-D13-1  3955 2515  131    3 1367 1726    2  251  274    51     0
## straw-its2-D13-2  6832 3674    0 1532 2225  450  169    0  107    47     0
## straw-its2-D13-3  1742 4111  113  232 2091  512 1254    0  141   224     0
## straw-its2-D14-1     0 5289    0  279 3189 2223    0  161 1009     0    80
## straw-its2-D14-2  4258 4360  170 1330 2965  531    0  332    0  2667    98
## straw-its2-D14-3  4050  917   91 1596  629 2401    8  140   82   331     0
## straw-its2-D14-4  4912  332  159 1199  263  282 1640  363    0  2000     0
## straw-its2-D14-5  6765 3002  149  128 1785  607    0    0    0     0     0
## straw-its2-D15-1     0    0    0    0    0  104    0    0 1106     0  2520
## straw-its2-D15-2  4877 1088    0  260    0  758 1890  127    0  3162    62
## straw-its2-D15-3  2756 5628   41 1188 3492 1104    0    0    0     0     0
##                  Seq12 Seq13 Seq14 Seq15 Seq16 Seq17 Seq18 Seq19 Seq20 Seq21
## straw-its2-D01-1     0     0     0  3670     0     0     0     0  1680     0
## straw-its2-D01-2     0     0     0  8045     0     0     0     0     0     0
## straw-its2-D01-3     0     0     0     0     0     0     0     0   951     0
## straw-its2-D03-1     0     0     0   128     0     0     0     0   153     0
## straw-its2-D03-2     0     0     0     0     0     0     0     0  3556     0
## straw-its2-D03-3     0     0     0   701     0     0     0  2637   798     0
## straw-its2-D05-1     0  4797     0     0  1224     0     0     0     0     0
## straw-its2-D05-2     8     0     0     0     0     0     0  3091     0     0
## straw-its2-D05-3     0     0     0     0     0     0     0  2125     0     0
## straw-its2-D05-4     0     0     0     0   711     0     0  2553     0     0
## straw-its2-D05-5     0     0     0   151  1203     0     0     0     0     0
## straw-its2-D07-1     0     0     0     0  2438     0     0     0     0    35
## straw-its2-D07-2     0   655     0     0   236     0     0     0     0  1995
## straw-its2-D07-3     0     0     0     0  2614     0     0     6   563   128
## straw-its2-D08-1   130     0     0     0  1443     0     0     0   190   333
## straw-its2-D08-2     0     0     0     0     0     0     0     0     0  1623
## straw-its2-D08-3     0     0     0   125    46  3073     0     0   370     0
## straw-its2-D10-1   251  1581     0     0     0     0     0     0     0     0
## straw-its2-D10-2   614     0     0     0     0  4871     0     0     0     0
## straw-its2-D10-3     0     0     0     0     0     0    62     0   375   339
## straw-its2-D10-4     0  1632     0   118     0     0     0     0    99     0
## straw-its2-D10-5     0  3763     0    77     0     0     0     0     0   190
## straw-its2-D12-1   201     0     0   112     0     0   369     0     0     0
## straw-its2-D12-2   591     0     0     0     0     0     0     0     0   469
## straw-its2-D12-3    91     0     0     0     0     0     0     0     0   139
## straw-its2-D13-1   738     0     0   242     0     0  1942     0     0     0
## straw-its2-D13-2  1547   507     0     0     0     0   731     0    42   109
## straw-its2-D13-3   878     0     0     0  1075     0  1701     0     0    44
## straw-its2-D14-1  1191     0     0     0     0     0  3062     0     0   695
## straw-its2-D14-2    27     0     0     0     2     0     0     0     0  2102
## straw-its2-D14-3  2395     0     0     0     0     0  1317     0   184    43
## straw-its2-D14-4  2579     0     0     0   775     0  1487     0     0     0
## straw-its2-D14-5   362   854     0     0     0     0     0     0     0   638
## straw-its2-D15-1     0     0 13776     0     0     0     0     0   839     0
## straw-its2-D15-2  1666     0     0     0   978  2146   986     0     0     0
## straw-its2-D15-3   813     0     0     0     0  2563     0     0     0   661
##                  Seq22 Seq23 Seq24 Seq25 Seq26 Seq27 Seq28 Seq29 Seq30 Seq31
## straw-its2-D01-1     0  1620     0     0   157     0     0     0     0     0
## straw-its2-D01-2     0  1042     0     0     0     0     0     0     0     0
## straw-its2-D01-3     0  1782  1479     0     0     0     0     0     0  3310
## straw-its2-D03-1     0   929     0     0     0     0     0    18   873     0
## straw-its2-D03-2     0   998     0     0     0     0     0     0   748     0
## straw-its2-D03-3     0   368  1003     0  1009    58     0     0   644     0
## straw-its2-D05-1     0     0     0     0    30     0     0     0    35     0
## straw-its2-D05-2     0   143     0    71   191     0     0     0   588   326
## straw-its2-D05-3     0    10     0    32   277     0     0     0  1261     0
## straw-its2-D05-4   929     0     0     0   179     0     0     0   123     0
## straw-its2-D05-5     0     0   462     0   185     0     0     0   310     0
## straw-its2-D07-1     0     0     0  1902   210     0     0     0   121    10
## straw-its2-D07-2  1392    37   241     0   202    19     7     0    99     0
## straw-its2-D07-3   218    11   171     0   183    29     0     0     0     0
## straw-its2-D08-1   123    15     0   499     0     0     0     0     0     4
## straw-its2-D08-2     0     0   211     0     0     0     0     0     0     0
## straw-its2-D08-3  1209    23   327  1918   373     0     0     0     0     0
## straw-its2-D10-1     0     0     0     4     0     0     0     0   103     0
## straw-its2-D10-2     0     0     0     0  1045    20     0    60   301     0
## straw-its2-D10-3   338     0     0   241   156  3581   409     0     0     0
## straw-its2-D10-4   345     8     0     0   330     0     0     0    29     0
## straw-its2-D10-5   607     0     0     0     0     0   573     0     0     0
## straw-its2-D12-1     0    57  1044  1724   297     0     0   663     0     0
## straw-its2-D12-2     0     0     0     0   169    27     0   540     0     0
## straw-its2-D12-3  1127    64     0     0  1121     0     0     0     0     0
## straw-its2-D13-1     0    56     0   445   166     0     0   786     0    99
## straw-its2-D13-2     0     0     0     0   292     0     0   263     0     0
## straw-its2-D13-3     0     0    95   170     0  1057     0     0     0     0
## straw-its2-D14-1     0    27     0     0   328     0     0  1049     0    30
## straw-its2-D14-2     0     7     0     0   293     0     0     0     0     0
## straw-its2-D14-3     0     0     0   488   392     0  4543   270     0     0
## straw-its2-D14-4   785     0     0   746     0     0     0   624     0     0
## straw-its2-D14-5   989    10     0     0   122     0   968   292     0     0
## straw-its2-D15-1     0  1100  3010     0     0     0     0     0     0  1053
## straw-its2-D15-2   534     0   222     0   230     0     0  1082     7     0
## straw-its2-D15-3     0     0     0     0     0  2769     0     0     0     0
##                  Seq32 Seq33 Seq34 Seq35 Seq36 Seq37 Seq38 Seq39 Seq40 Seq41
## straw-its2-D01-1     0     0     0     0     0   955     0     0  1030     0
## straw-its2-D01-2     0     0     0     0     0   355     0     0  1320     0
## straw-its2-D01-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D03-1     0     0   593  1300     0    78   857     0   564  2184
## straw-its2-D03-2     0   879     0     0     0     0  1217     0   598     0
## straw-its2-D03-3     0     0  2004     0     0     0     0     0   459   937
## straw-its2-D05-1     0  1839     0  2588     0     0  2286     0     0     0
## straw-its2-D05-2     0   220     0     0     0   333     0     0     0     0
## straw-its2-D05-3     0  1608     0     0     0   198     0     0     0     0
## straw-its2-D05-4     0     0  1946     0     0    56     0     0    55     0
## straw-its2-D05-5     0     0     0     0     0     0     0     0     0     0
## straw-its2-D07-1     0     0     0     0     0    96     0     0     0     5
## straw-its2-D07-2    18     0     0     0     0     0     0   676     0     0
## straw-its2-D07-3   437     0     0     0     0     0     0   139    53     0
## straw-its2-D08-1     0     0     0     0    45  1118     0     0     0     0
## straw-its2-D08-2     0     0     0     0     4     0     0   740     0     0
## straw-its2-D08-3     0     0     0     0     0     0     0   389     0     0
## straw-its2-D10-1     0     0     0     0     0     0     0     0     0     0
## straw-its2-D10-2     0     0     0     0   123   289     0   280     0     0
## straw-its2-D10-3    38     0     0     0     0     0     0     0     0     0
## straw-its2-D10-4     0     0     0     0    84   114     0     0     0    47
## straw-its2-D10-5     0     0     0     0     0     0     0   311     0     0
## straw-its2-D12-1     0     0     0     0     0     0     0     0    97    53
## straw-its2-D12-2  1305     0     0     0   235   588     0     0     0     0
## straw-its2-D12-3   928     0     0   625   561     0     0   557     0   211
## straw-its2-D13-1     0     0     0     0   824     0     0     0     0   152
## straw-its2-D13-2   185     0     0     0   287    32     0     0     0     0
## straw-its2-D13-3   471     0     0     0   277     0     0     0     0     0
## straw-its2-D14-1     0     0     0     0    87     0     0     0     0    26
## straw-its2-D14-2   111     0     0     0     3     0     0     0     4   410
## straw-its2-D14-3     0     0     0     0  1148     0     0     0    16    68
## straw-its2-D14-4   646     0     0     0   160     0     0     0     0     0
## straw-its2-D14-5   514     0     0     0     0   204     0   859     0     0
## straw-its2-D15-1     0     0     0     0    17     0     0     0     0     0
## straw-its2-D15-2     0     0     0     0     0     0     0   397     0    91
## straw-its2-D15-3     0     0     0     0   574     0     0     0     0     0
##                  Seq42 Seq43 Seq44 Seq45 Seq46 Seq47 Seq48 Seq49 Seq50 Seq51
## straw-its2-D01-1     0     0     0   653     0   401     0     0     0     0
## straw-its2-D01-2     0     0     0   537     0     0     0     0     0     0
## straw-its2-D01-3     0     0     0   779   285     0     0     0     0     0
## straw-its2-D03-1     0  1383     0     0     0     0     0     0     0     0
## straw-its2-D03-2     0   300     0     0     0  1162     0     0     0     0
## straw-its2-D03-3     0  1107     0   400     0     0     0     0     0     0
## straw-its2-D05-1   336     0     0     0     0     0     0   279     0     0
## straw-its2-D05-2     0   118     0    41     0     0     0     0   289     0
## straw-its2-D05-3     0   440     0     0     0     0     0     0     0     0
## straw-its2-D05-4   213   147     0     0     0     0     0   197     0     0
## straw-its2-D05-5   287   522     0   127     0     0     0   316     0     0
## straw-its2-D07-1   768     0     0    26     0     0     0   585   392     0
## straw-its2-D07-2   107     0    11     0     0     0     4     0    62     0
## straw-its2-D07-3   878     0   306     0     0     0   315   709   326     0
## straw-its2-D08-1   495     0     0     0   176     0     0   334   464     0
## straw-its2-D08-2     0     0     0     0     0     0     0     0     0     0
## straw-its2-D08-3     0     0     0     0     0     0     0     0   249     0
## straw-its2-D10-1     0     0     0     0     0     0     0     0     0     0
## straw-its2-D10-2     0     0     0   104   176     0     0     0     0     0
## straw-its2-D10-3     0     0    42    30     0   402     0     0    56     0
## straw-its2-D10-4     0     0    11   118     0     0     0     0     0     0
## straw-its2-D10-5     0     0     0   134     0   199    11     0     0     0
## straw-its2-D12-1     0     0     0   169     0     0     0     0   379     0
## straw-its2-D12-2     0     0   715     0     0   238   806     0     0     0
## straw-its2-D12-3     0     0   814   133    79   791   622     0   146     0
## straw-its2-D13-1     0     0     0     0     0     0     0     0     0  2875
## straw-its2-D13-2     0     0   218    30     0     0   200     0     0     0
## straw-its2-D13-3   267     0   354     0  1241     0   422   341   164     0
## straw-its2-D14-1     0     0     0    73   429   236     0     0   295     0
## straw-its2-D14-2     0     0   110     0     0     0   132     0     0     0
## straw-its2-D14-3     0     0   322     0   426     0   273     0     0     0
## straw-its2-D14-4   399     0   494     0     0     0   389     0   181     0
## straw-its2-D14-5     0     0   336     0     0     0     0     0     0     0
## straw-its2-D15-1     0     0     0   197     0     0     0     0     0     0
## straw-its2-D15-2   285     0     0     0     0     0     0   254     0     0
## straw-its2-D15-3     0     0     0     0   704     0     0     0     0     0
##                  Seq52 Seq53 Seq54 Seq55 Seq56 Seq57 Seq58 Seq59 Seq60 Seq61
## straw-its2-D01-1     0     0     0     0     0     0     0     0     0     0
## straw-its2-D01-2     0     0     0     0     0     0     0     0     0     0
## straw-its2-D01-3     0     0     0     0     0     0  2297     0     0     0
## straw-its2-D03-1   343   100     0     0     0     0     0    99     0     0
## straw-its2-D03-2     0     0     0     0  2314     0     0     0     0     0
## straw-its2-D03-3   490   437     0     0     0     0     0     0     0     0
## straw-its2-D05-1     0     0     0     0     0     0     0     0  1334     0
## straw-its2-D05-2     0   479     0     0     0     0     0     0     0  2180
## straw-its2-D05-3   963   659     0     0     0     0     0     0     0     0
## straw-its2-D05-4     0   163   284     0     0     0     0   548     0     0
## straw-its2-D05-5   718   276     0  2321     0  2313     0     0     0     0
## straw-its2-D07-1    52   114   643     0     0     0     0     0     0     0
## straw-its2-D07-2     0     0   177     0     0     0     0    19     0     0
## straw-its2-D07-3     0    32     0     0     0     0     0     0     0     0
## straw-its2-D08-1     0     0  1253     0     0     0     0     0     0     0
## straw-its2-D08-2     0     0     0     0     0     0     0     0     0     0
## straw-its2-D08-3     0   154     0     0     0     0     0   440     0     0
## straw-its2-D10-1     0     0     0     0     0     0     0   269     0     0
## straw-its2-D10-2     0   193     0     0     0     0     0     0     0     0
## straw-its2-D10-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D10-4     0     0     0     0     0     0     0     0   439     0
## straw-its2-D10-5    35     0     0     0     0     0     0     0     0     0
## straw-its2-D12-1     0     0    43     0     0     0     0     0     0     0
## straw-its2-D12-2     0     0    53     0     0     0     0    31     0     0
## straw-its2-D12-3    15     0     0     0     0     0     0   637     0     0
## straw-its2-D13-1    19     0     0     0     0     0     0     0     0     0
## straw-its2-D13-2     0     0     0     0     0     0     0     0     0     0
## straw-its2-D13-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D14-1     0     0     0     0     0     0     0     0     0     0
## straw-its2-D14-2     0     0     0     0     0     0     0     0     0     0
## straw-its2-D14-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D14-4     0     0     0     0     0     0     0     0     0     0
## straw-its2-D14-5     0     0     0     0     0     0     0     0   431     0
## straw-its2-D15-1     0     0     0     0     0     0     0    99     0     0
## straw-its2-D15-2     0     0     0     0     0     0     0    35     0     0
## straw-its2-D15-3     0     0     0     0     0     0     0    31     0     0
##                  Seq62 Seq63 Seq64 Seq65 Seq66 Seq67 Seq68 Seq69 Seq70 Seq71
## straw-its2-D01-1     0   846     0     0     0     0     0     0   105     0
## straw-its2-D01-2     0     0     0     0     0     0     0     0     0     0
## straw-its2-D01-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D03-1     0   629     0     0     0     0   154     0   159     0
## straw-its2-D03-2     0   493     0     0     0     0   116   556     0     0
## straw-its2-D03-3     0     0     0     0     0     0    39     0    94     0
## straw-its2-D05-1     0     0     0     0    73     0    16    24     0     0
## straw-its2-D05-2     0     0     0     0   505     0   785   147     0     0
## straw-its2-D05-3     0    18     0     0     0     0   385   106     0     0
## straw-its2-D05-4     0     0     0     0     0     0   381    22     0     0
## straw-its2-D05-5     0    61     0     0     0     0     9     0   106     0
## straw-its2-D07-1     0     0     0  1255   824     0     0    67     0     0
## straw-its2-D07-2     0    67     0     0     0     0     0     0     0     0
## straw-its2-D07-3   234     0     0     0     0     0     0     0     0     0
## straw-its2-D08-1     0     0     0     0     0     0     2   406     0     0
## straw-its2-D08-2     0     0     0    90     0     0     3     0    71     0
## straw-its2-D08-3     0     0     0     0     0     0     5     0     0     0
## straw-its2-D10-1     0     0  2127     0     0   608     0     0     0     0
## straw-its2-D10-2     0     0     0     0     0     0     7     0     0     0
## straw-its2-D10-3    16     0     0     0     0     0     0     0     0     0
## straw-its2-D10-4     0     0     0     0   436     0     0     0     0  1703
## straw-its2-D10-5    21     0     0     0     0    34     0     0     0     0
## straw-its2-D12-1     0     0     0     0     0   957     2   228   502     0
## straw-its2-D12-2   588    30     0     0    10     0     0     0     0     0
## straw-its2-D12-3   486     0     0   238     0     0     0     0   372     0
## straw-its2-D13-1     0     0     0     0     0     0     0     0   356     0
## straw-its2-D13-2   111     0     0     0     0     0     0     0     0     0
## straw-its2-D13-3     0     0     0    27     0    85     0     0     0     0
## straw-its2-D14-1     0     0     0     0     0     0     0     0     0     0
## straw-its2-D14-2    74     0     0   431     0     0     0     0     0     0
## straw-its2-D14-3   176     0     0     0     0     0     0   190     0     0
## straw-its2-D14-4   278     0     0     0     0     0     0    34     0     0
## straw-its2-D14-5   193     0     0     0   132     0     0    60     0     0
## straw-its2-D15-1     0     0     0     0     0   232     0     0     0     0
## straw-its2-D15-2     0     0     0    21    37     0     0     0     0     0
## straw-its2-D15-3     0     0     0     0    17     0     0    49     0     0
##                  Seq72 Seq73 Seq74 Seq75 Seq76 Seq77 Seq78 Seq79 Seq80 Seq81
## straw-its2-D01-1     0     0     0     0     0     0     0     0     0     0
## straw-its2-D01-2     0     0     0     0     0     0     0     2  1358     0
## straw-its2-D01-3     0     0     0     2     0     0     0     1     0     0
## straw-its2-D03-1     0     0     0     0     0     0     0   100     0     0
## straw-its2-D03-2     0     0     0     7     0     0     0    95     0     0
## straw-its2-D03-3     0     0     0     0     0     0     0    40     0     0
## straw-its2-D05-1     0     0     0   136     0     0     0    53     0     0
## straw-its2-D05-2     0     0     0    17     0     0     0   403     0     0
## straw-its2-D05-3     0     0     0     8     0  1534     0   268     0     0
## straw-its2-D05-4     0     0     0   986     0     0     0   153     0     0
## straw-its2-D05-5  1656     0     0     2     0     0     0   205     0     0
## straw-its2-D07-1     0     0     0     0     0     0     0     0     0   263
## straw-its2-D07-2     0     0     0     0     0     0     0     9     0   408
## straw-its2-D07-3     0     0     0     0    63     0     0     0     0     0
## straw-its2-D08-1     0     0   129     8   127     0     0     0     0     0
## straw-its2-D08-2     0     0   299     0     0     0     0     0     0     0
## straw-its2-D08-3     0     0     0     5     0     0     0     6     0     0
## straw-its2-D10-1     0    57     0    61     0     0     0     2     0     0
## straw-its2-D10-2     0   277     0    95   176     0     0    13     0     5
## straw-its2-D10-3     0     0     0     0     0     0    21     0     0     0
## straw-its2-D10-4     0     0     0     0    97     0     0     6     0     0
## straw-its2-D10-5     0    24     0     0     0     0    30     0     0     0
## straw-its2-D12-1     0    38     0    42     0     0     0     0     0     0
## straw-its2-D12-2     0   146   415    13     0     0     0     0     0     0
## straw-its2-D12-3     0    58     0    41     0     0   518     4     0     0
## straw-its2-D13-1     0   207     0    18     0     0     0     0     0     0
## straw-its2-D13-2     0     0    22     9     0     0   105     2     0     0
## straw-its2-D13-3     0     0     0     5     0     0     0     0     0     0
## straw-its2-D14-1     0     0     0    13   648     0     0     0     0     0
## straw-its2-D14-2     0   145   392     0     0     0    98     0     0     0
## straw-its2-D14-3     0   136     0    25   222     0   187     0     0     0
## straw-its2-D14-4     0     0     0    33     0     0   335     0     0     0
## straw-its2-D14-5     0   335     0    20     0     0   240     0     0     0
## straw-its2-D15-1     0     0     0    39     0     0     0     0     0     0
## straw-its2-D15-2     0    79   286     0   110     0     0     0     0     0
## straw-its2-D15-3     0   103    57     0   120     0     0     0     0   661
##                  Seq82 Seq83 Seq84 Seq85 Seq86 Seq87 Seq88 Seq89 Seq90 Seq91
## straw-its2-D01-1     0     0     0     0     0     0     0     0   366     0
## straw-its2-D01-2     0     0     0     0     0     0     0     0     0     0
## straw-its2-D01-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D03-1     0     0     0     0     0     0     0     0     0     0
## straw-its2-D03-2     0     0     0     0     0     0     0     0   724     0
## straw-its2-D03-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D05-1     0     0     0     0    97     0     0     0     0     0
## straw-its2-D05-2     0  1242     0     0     0     0     0     0     0     0
## straw-its2-D05-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D05-4     0     0     0     0    70     0     0     0     0     0
## straw-its2-D05-5     0     0     0     0    86  1166     0     0     0     0
## straw-its2-D07-1     0     0     0     0   247     0     0     0     0     0
## straw-its2-D07-2     0     0     0    12    25     0     0    84     0     0
## straw-its2-D07-3  1273     0     0   244   284     0     0   656     0     0
## straw-its2-D08-1     0     0     0     0   148     0     0     0     0     0
## straw-its2-D08-2     0     0     0     0     0     0     0     0     0   552
## straw-its2-D08-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D10-1     0     0     0     0     0     0     0     0     0     0
## straw-its2-D10-2     0     0     0     0     0     0     0   118     0     0
## straw-its2-D10-3     0     0     0     0     0     0     0     0     0   292
## straw-its2-D10-4     0     0     0     0     0     0     0     0     0     0
## straw-its2-D10-5     0     0     0     0     0     0  1106     0     0     0
## straw-its2-D12-1     0     0     0     0     0     0     0     0     0     0
## straw-its2-D12-2     0     0     0   672     0     0     0     0     0     0
## straw-its2-D12-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D13-1     0     0  1235     0     0     0     0     0     0     0
## straw-its2-D13-2     0     0     0     0     0     0     0    20     0     0
## straw-its2-D13-3     0     0     0   290    76     0     0     0     0     0
## straw-its2-D14-1     0     0     0     0     0     0     0   226     0     0
## straw-its2-D14-2     0     0     0     0     0     0     0     0     0     0
## straw-its2-D14-3     0     0     0     0     0     0     0     0     0     0
## straw-its2-D14-4     0     0     0     0    61     0     0     0     0     0
## straw-its2-D14-5     0     0     0     0     0     0     0     0     0    86
## straw-its2-D15-1     0     0     0     0     0     0     0     0     0     0
## straw-its2-D15-2     0     0     0     0    88     0     0     0     0     0
## straw-its2-D15-3     0     0     0     0     0     0     0     0     0   134
##                  Seq92 Seq93 Seq94 Seq95 Seq96 Seq97 Seq98 Seq99 Seq100 Seq101
## straw-its2-D01-1     0     0     0   409     0     0   951     0    224      0
## straw-its2-D01-2     0     0     0     0     0     0     0     0      0      0
## straw-its2-D01-3     0     0     0   607     0     0     0     0      0      0
## straw-its2-D03-1     0     0     0     0   141     0     0    24     82     37
## straw-its2-D03-2     0     0     0     0   213     0     0    24    293      0
## straw-its2-D03-3     0     0     0     8    14     0     0     3      0      0
## straw-its2-D05-1    72     0     0     0     0     7     0     4      0      0
## straw-its2-D05-2     0     0     0     0   521     0     0   412     45      0
## straw-its2-D05-3     0     0     0     0   132     0     0     0     60      0
## straw-its2-D05-4     0     0     0     0     0     0     0   396     25      0
## straw-its2-D05-5     0     0     0     0     0     0     0    42      0      0
## straw-its2-D07-1   338    25     0     0     0     0     0     0      0      0
## straw-its2-D07-2    24     0     0     0     0   112     0     0      0      0
## straw-its2-D07-3   414    89     0     0     0     0     0     0      0      0
## straw-its2-D08-1   210   150     0     0     0     0     0     2      0      0
## straw-its2-D08-2     0     0     0     0     0     0     0     0      0      0
## straw-its2-D08-3     0   125     0     0     0     0     0     0     48      0
## straw-its2-D10-1     0     0     0     0     0     0     0    20      0      0
## straw-its2-D10-2     0    17    34     1     0     0     0    13      0     33
## straw-its2-D10-3     0     0     0     0     0     0     0     0      0      0
## straw-its2-D10-4     0    27     0     0     0     0     0     1      0     32
## straw-its2-D10-5     0     0     0     0     0     0     0     0      0      5
## straw-its2-D12-1     0   113    32     0     0     0     0     0      0    454
## straw-its2-D12-2     0     0   111     3     0     0     0     3     94     34
## straw-its2-D12-3     0   112     0     0     0     0     0     0      0     21
## straw-its2-D13-1     0     0   162     0     0     0     0     0      0    110
## straw-its2-D13-2     0     0     0     0     0     0     0     0      0      0
## straw-its2-D13-3     0     0    64     0     0     0     0     0      0     36
## straw-its2-D14-1     0     0   128     0     0     0     0     0      0      0
## straw-its2-D14-2     0    38     0     0     0     0     0     0      0      0
## straw-its2-D14-3     0    95     0     0     0     0     0     0      0     54
## straw-its2-D14-4     0   202   147     0     0   847     0     1      0     13
## straw-its2-D14-5     0     7   142     0     0     0     0     0     41      0
## straw-its2-D15-1     0     0     0     0     0     0     0     0      0     80
## straw-its2-D15-2     0    30   220     0     0     0     0     0      0      0
## straw-its2-D15-3     0    10     0     0     0     0     0     0     18     15
##                  Seq102 Seq103 Seq104 Seq105 Seq106 Seq107 Seq108 Seq109 Seq110
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      3      0      0    874      0    839      0      0      0
## straw-its2-D03-1     20      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      5      0      0      0      0      0      0      0      0
## straw-its2-D05-1    130      0      9      0      0      0      0      0      0
## straw-its2-D05-2    139      0      0      0      0      0      0      0      0
## straw-its2-D05-3      5      0      0      0      0      0      0      0      0
## straw-its2-D05-4    231      0      0      0    715      0      0      0      0
## straw-its2-D05-5      3      0      0      0    127      0      0      0      0
## straw-its2-D07-1      0      0     16      0      0      0      0      0      0
## straw-its2-D07-2      0      8      0      0      0      0      2      0      0
## straw-its2-D07-3      0    182    159      0      0      0     86      0    797
## straw-its2-D08-1      6      0    241      0      0      0      0      0      0
## straw-its2-D08-2      5      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0     60      0      0
## straw-its2-D10-1      8      0      0      0      0      0      0      0      0
## straw-its2-D10-2    163      0    213      0      0      0    106      0      0
## straw-its2-D10-3     24     18     33      0      0      0      0      0      0
## straw-its2-D10-4     12      0    116      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      7      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0     70     10      0      0      0    165      0      0
## straw-its2-D12-3      0      0      0      0      0      0    119    529      0
## straw-its2-D13-1      7      0      0      0      0      0      0      0      0
## straw-its2-D13-2      3    104      0      0      0      0      0      0      0
## straw-its2-D13-3      0    236      0      0      0      0     81      0      0
## straw-its2-D14-1     14      0      0      0      0      0      0      0      0
## straw-its2-D14-2      8      0      0      0      0      0     21     82      0
## straw-its2-D14-3      8      0     38      0      0      0      0    196      0
## straw-its2-D14-4      9     38      6      0      0      0     85      0      0
## straw-its2-D14-5     36    244     27      0      0      0      0      0      0
## straw-its2-D15-1     59      0      0      0      0      0      0      0      0
## straw-its2-D15-2     12      0      0      0      0      0     40      0      0
## straw-its2-D15-3      6      0     17      0      0      0     39      0      0
##                  Seq111 Seq112 Seq113 Seq114 Seq115 Seq116 Seq117 Seq118 Seq119
## straw-its2-D01-1      0      0      0      0      0    316      0      0    614
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0    775      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0    103
## straw-its2-D03-2      0      0      0      0      0      0    740      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0    432      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0    168      0      0      0      0      0
## straw-its2-D07-2      0    796      0     21      0      0      0      0      0
## straw-its2-D07-3      0      0      0    443      0      0      0     34      0
## straw-its2-D08-1      0      0      0     95      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0     47      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0     14      0
## straw-its2-D12-1    797      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0    418      0
## straw-its2-D12-3      0      0      0      0    474      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0    288      0      0    257      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq120 Seq121 Seq122 Seq123 Seq124 Seq125 Seq126 Seq127 Seq128
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      9      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0    283      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0    463      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0    287      0     92    660      0      0
## straw-its2-D05-4      0     45      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0     44      0      0     49      0      0      0     48
## straw-its2-D07-3      0      0      3      0      0      0      0      0    561
## straw-its2-D08-1      0     91      0      0      0    363      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0     11      0      0      0      0
## straw-its2-D10-1      0      0      0      0    423      0      0      0      0
## straw-its2-D10-2      0      0      8      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0     12     32      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0     43      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0     90      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0    198      0    148     87      0      0      0
## straw-its2-D13-3      0      0    259      0      0      0      0      0      0
## straw-its2-D14-1      0      0     48      0      0     10      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0     20      0      0      0      0      0      0
## straw-its2-D14-4      0      0     93      0      0     77      0      0      0
## straw-its2-D14-5    701      0      0      0      0     32      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0     39      0      0      0      0    622      0
##                  Seq129 Seq130 Seq131 Seq132 Seq133 Seq134 Seq135 Seq136 Seq137
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      8      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0    520      0      0      0     40
## straw-its2-D05-1      0      0      0    342      0      0      0      0    136
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0    570      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      7      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0    213    268
## straw-its2-D08-1      0      0      0      3      0      0      0     18      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0     52      0
## straw-its2-D10-1      0      0    552     61      0      0      0      0      0
## straw-its2-D10-2      0      0      0      4      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0     58      0
## straw-its2-D10-5      0      0      0      2      0      0    504      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0     38      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0     23      0      0      0      0      0
## straw-its2-D13-2     58      0      0     11      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0     56
## straw-its2-D14-1    382      0      0     40      0      0      0      0      0
## straw-its2-D14-2    168      0      0      1      0    310      0     65      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0     26      0    207      0      0      0
## straw-its2-D14-5      0      0      0      1      0      0      0     58      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq138 Seq139 Seq140 Seq141 Seq142 Seq143 Seq144 Seq145 Seq146
## straw-its2-D01-1      0      0      8      0      0      0      0      0    415
## straw-its2-D01-2      0      0      0      0      0      0      0    446      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0    105      0     88      0      0      0      0
## straw-its2-D03-2      9      0     21      0      0      0      0      0      0
## straw-its2-D03-3      0      0     13      0      0      0      0      0      0
## straw-its2-D05-1      0      0      4      0      0      0      0      0      0
## straw-its2-D05-2    200      0      2      0      0      0      0      0      0
## straw-its2-D05-3      0      0      4      0      0      0      0      0      0
## straw-its2-D05-4    210      0     84      0      0      0      0      0      0
## straw-its2-D05-5     24      0      0    470      0      0      0      0      0
## straw-its2-D07-1      0      0      4      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0     16      0    372      0      0      0      0
## straw-its2-D08-3      5      0      2      0      0      0      0      0      0
## straw-its2-D10-1     17      0    170      0      0      0      0      0      0
## straw-its2-D10-2     10      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      5      0      0      0      0      0      0
## straw-its2-D10-4      0    472      3      0      0      0    448      0      0
## straw-its2-D10-5      0      0      3      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      2      0      0      0      0      0      0
## straw-its2-D12-3      0      0      1      0      0      0      0      0      0
## straw-its2-D13-1      0      0      4      0      0      0      0      0      0
## straw-its2-D13-2      0      0     19      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0    457      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      1      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq147 Seq148 Seq149 Seq150 Seq151 Seq152 Seq153 Seq154 Seq155
## straw-its2-D01-1      0      0      0      4      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      3
## straw-its2-D03-1    128      0      0     17      0      0      0      0      0
## straw-its2-D03-2    132      0      0      4      0     19      0      0      0
## straw-its2-D03-3      0      0    400      8      0     65      0      0      0
## straw-its2-D05-1      0      0      0      3      0      1      0      0      0
## straw-its2-D05-2     80      0      0      0      0     59      0      0      0
## straw-its2-D05-3     68      0      0     72      0     11      0      0      0
## straw-its2-D05-4      0      0      0      5      0    102      0      6      0
## straw-its2-D05-5      0      0      0      0      0     42      0      0      0
## straw-its2-D07-1      0      0      0     16      0      0      0      0      0
## straw-its2-D07-2      0      0      0     13      0      0      0    106      0
## straw-its2-D07-3      0      0      0      0    393      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0     29      0    202      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      3
## straw-its2-D10-2      0      0      0     12      0      6      0      0      4
## straw-its2-D10-3      0      0      0      5      0     11      0      0      0
## straw-its2-D10-4      0      0      0      0      0     36      0      0      1
## straw-its2-D10-5      0      0      0      0      0      1      0      0      1
## straw-its2-D12-1      0      0      0     11      0      0      0      0     57
## straw-its2-D12-2      0      0      0      0      0      0      0      0     34
## straw-its2-D12-3      0      0      0     66      0      3      0     68     25
## straw-its2-D13-1      0      0      0      0      0      0    385      0     29
## straw-its2-D13-2      0      0      0      0      0      0      0      0     69
## straw-its2-D13-3      0      0      0      0      0      0      0      0      8
## straw-its2-D14-1      0      0      0     57      0      0      0      0     16
## straw-its2-D14-2      0      0      0     27      0      0      0      0     17
## straw-its2-D14-3      0      0      0     35      0      0      0      0     34
## straw-its2-D14-4      0    407      0      0      0      4      0      0      8
## straw-its2-D14-5      0      0      0      0      0      2      0      0     16
## straw-its2-D15-1      0      0      0     12      0      0      0      0     14
## straw-its2-D15-2      0      0      0      8      0      0      0      0     25
## straw-its2-D15-3      0      0      0     20      0      0      0      0     16
##                  Seq156 Seq157 Seq158 Seq159 Seq160 Seq161 Seq162 Seq163 Seq164
## straw-its2-D01-1      0      0    349      0      0    198      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      7      0      0     58      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      1      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0     18      0      0      0
## straw-its2-D05-2      0    144      0      0      0      6      0      0      0
## straw-its2-D05-3      0      0      0    357      0     53      0      0      0
## straw-its2-D05-4      0      0      0      0      0      9      0      0      0
## straw-its2-D05-5      0      0      3      0      0      0      0      0      0
## straw-its2-D07-1    376      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0     72      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      6      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      4
## straw-its2-D10-4      0    124      0      0      0      0      0      0      7
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0    343    338      0
## straw-its2-D13-2      0      0      0      0      0      2      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0     25      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0    356      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0    314
## straw-its2-D15-1      0      0      0      0      0      0      0      0      6
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq165 Seq166 Seq167 Seq168 Seq169 Seq170 Seq171 Seq172 Seq173
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0    129      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0     58      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0     13      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0     15      0      0
## straw-its2-D07-2      0      0      0      0      0    287      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0    128      0      0      0      0      0      0
## straw-its2-D10-2      0     50      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0     68      0      0
## straw-its2-D10-4      0      0      0      0      0      0     38      0    298
## straw-its2-D10-5      0    108      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0     57      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0    301      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0     81      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1    322      9      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0     38      0      0      0      0    122    300      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0    302      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0     31      0      0      0      0      0      0      0
##                  Seq174 Seq175 Seq176 Seq177 Seq178 Seq179 Seq180 Seq181 Seq182
## straw-its2-D01-1    297    177      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      2    147      0
## straw-its2-D03-1      0      0      0      0      0      0      0     89      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0     61      0      0      0      0      0     11      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0    293      0      0      0    173      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      5      0      0
## straw-its2-D05-5      0      0      0      0      0    285      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0     39      0      0      0      0      0     28      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0     25      0    274
## straw-its2-D08-3      0      0      0      0      0      0      2      0      0
## straw-its2-D10-1      0      0      0    291    186      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0     58      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0     15      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0     19      0      1      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0     25      0      0      1      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      7      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      1      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0     17      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0     50      0      0
##                  Seq183 Seq184 Seq185 Seq186 Seq187 Seq188 Seq189 Seq190 Seq191
## straw-its2-D01-1      8      0      0      0      0      0      0      0      0
## straw-its2-D01-2    216      0      0      0      0      0      0      0      0
## straw-its2-D01-3     30      1      0      0    254      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      5      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      3      0      0      0      0      0      0      0      0
## straw-its2-D05-4      1      1      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      1      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      2      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      4     34      0      0      0      0      0     25     26
## straw-its2-D10-2      2     23      0      0      0      0    248      9     62
## straw-its2-D10-3      0     46      0      0      0      0      0     14      5
## straw-its2-D10-4      0     15      0      0      0      0      0     30     43
## straw-its2-D10-5      0      0    263      0      0      0      0     77      5
## straw-its2-D12-1      0     12      0      0      0      0      0      0      0
## straw-its2-D12-2      0     37      0      0      0      0      0      0      7
## straw-its2-D12-3      0     63      0      0      0      0      0      6     57
## straw-its2-D13-1      0      0      0    255      0      0      0      2      0
## straw-its2-D13-2      0     13      0      0      0      0      0      1     21
## straw-its2-D13-3      0      0      0      0      0    254      0      2      3
## straw-its2-D14-1      0      4      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0     11      3
## straw-its2-D14-3      1      9      0      0      0      0      0      6      1
## straw-its2-D14-4      0      0      0      0      0      0      0     15     11
## straw-its2-D14-5      0      0      0      0      0      0      0     35      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      2      0      0      0      0      0      0      8      0
## straw-its2-D15-3      0      6      0      0      0      0      0      2      1
##                  Seq192 Seq193 Seq194 Seq195 Seq196 Seq197 Seq198 Seq199 Seq200
## straw-its2-D01-1      0     31      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3    244      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0     88      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      6      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0     88      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      2      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0     13      0      0      0      0      0
## straw-its2-D08-2      0      0      0      3      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0    238      2      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0     10    219      0      0      0      0
## straw-its2-D12-1      0      9      0      1      0      0      0      0      0
## straw-its2-D12-2      0      0      0     16      0      0      0      0      0
## straw-its2-D12-3      0      2      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0     53      0      0      0      0      0
## straw-its2-D13-2      0      0      0     39      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0     15      0      0      0    207      0      0      0
## straw-its2-D14-2      0      0      0     72      0      0      0      0      0
## straw-its2-D14-3      0      4      0      0      0      0    204      0      0
## straw-its2-D14-4      0      0      0      6      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      1      0      0      0      0    196
## straw-its2-D15-2      0      2      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      1      0      0      0    201      0
##                  Seq201 Seq202 Seq203 Seq204 Seq205 Seq206 Seq207 Seq208 Seq209
## straw-its2-D01-1      0      0      0      0     85      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0    191    187      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0     56      0      0      0    175
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2     52      0      0      0      0      0      0      0      0
## straw-its2-D05-3    140      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0    178      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      2      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0    179      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0     18      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0    180      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0     24      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0    186      0      0      0      0      0
##                  Seq210 Seq211 Seq212 Seq213 Seq214 Seq215 Seq216 Seq217 Seq218
## straw-its2-D01-1    170      0      0      0      0      7      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0    170      0      0      0     14      0      0      0
## straw-its2-D03-2      0      0      0      0      0     43      0      0      0
## straw-its2-D03-3      0      0      0      0      0      6      0      0      0
## straw-its2-D05-1      0      0      0      0      0      3      0      0      0
## straw-its2-D05-2      0      0    164      0      0     31      0    147      0
## straw-its2-D05-3      0      0      0      0      0     23      0      0      0
## straw-its2-D05-4      0      0      0      0      9      0      0      0    145
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0    101      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0    155      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      5      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      3      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0    163      1      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0     43      2      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      2      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      3      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      3      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      3     20      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq219 Seq220 Seq221 Seq222 Seq223 Seq224 Seq225 Seq226 Seq227
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      7      0      0      0    138      0      0      0
## straw-its2-D03-2      0      2      0      0      0      0      0      0      0
## straw-its2-D03-3      0      1      0      0      0      0      0      0      0
## straw-its2-D05-1      0      9      0      0      0      0     14      0      0
## straw-its2-D05-2      0      1      0      0      0      0      4      0      0
## straw-its2-D05-3      0      4      0    141      0      0      3      0      0
## straw-its2-D05-4      0     55      0      0      0      0     46      0      0
## straw-its2-D05-5      0      1      0      0      0      0      1      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0     73      0
## straw-its2-D07-3      0      0      0      0    141      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      3      0      0
## straw-its2-D10-1      0      7      0      0      0      0      6      0      0
## straw-its2-D10-2      0     23      0      0      0      0     35      0      0
## straw-its2-D10-3      0      5      0      0      0      0      1      0      0
## straw-its2-D10-4    145      0      0      0      0      0      1      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      2      0      0      0      0      0      0      0
## straw-its2-D12-2      0      6      0      0      0      0      1      0      0
## straw-its2-D12-3      0      4      0      0      0      0      2      0    135
## straw-its2-D13-1      0      1      0      0      0      0      1     25      0
## straw-its2-D13-2      0      1      0      0      0      0      1      0      0
## straw-its2-D13-3      0      1      0      0      0      0      2      0      0
## straw-its2-D14-1      0      3      0      0      0      0      3     19      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      3      0      0      0      0      2      0      0
## straw-its2-D14-4      0      1      0      0      0      0      1      0      0
## straw-its2-D14-5      0      4      0      0      0      0      3      0      0
## straw-its2-D15-1      0      3      0      0      0      0      4     18      0
## straw-its2-D15-2      0      0    142      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      1      0      0
##                  Seq228 Seq229 Seq230 Seq231 Seq232 Seq233 Seq234 Seq235 Seq236
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0     15      0
## straw-its2-D03-1      0      0      0      0      0      0    122      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0    110      5      0      0      0
## straw-its2-D05-3      0      0      0      0      0     59      0      0      0
## straw-its2-D05-4      0      0      0      0      4      3      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      4      0      0     17      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2     98      0      0      8      0     26      0      1      0
## straw-its2-D10-3      0      0     91      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0     15      5      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      1      0
## straw-its2-D12-1      0      0      0      4      0      6      0      8      0
## straw-its2-D12-2     35      0      0      0      0      0      0      1      0
## straw-its2-D12-3      0      0      0     30      0      0      0      3      0
## straw-its2-D13-1      0    131      0     14      0      0      0     15      0
## straw-its2-D13-2      0      0      0      0      0      0      0      5      0
## straw-its2-D13-3      0      0      0      0      0      0      0      2      0
## straw-its2-D14-1      0      0      0      0      0      0      0     13      0
## straw-its2-D14-2      0      0      0      0      0      9      0      0      0
## straw-its2-D14-3      0      0      0     22      0      7      0      7      0
## straw-its2-D14-4      0      0      0     13      0      0      0     15      0
## straw-its2-D14-5      0      0     11      9      0      0      0      8      0
## straw-its2-D15-1      0      0      0      0      0      0      0      6      0
## straw-its2-D15-2      0      0      0      0      0      8      0      3    119
## straw-its2-D15-3      0      0     26     10      0      0      0      0      0
##                  Seq237 Seq238 Seq239 Seq240 Seq241 Seq242 Seq243 Seq244 Seq245
## straw-its2-D01-1      0      0      0      0      0    111      0      0     31
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0     51      0      0      0      0    110      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0     29
## straw-its2-D03-2      0     13      0    113      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      4
## straw-its2-D05-1      0      0     53      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      3
## straw-its2-D05-3      0      0      0      0      0      0      0      0      3
## straw-its2-D05-4    118      0      0      0      0      0      0      0     12
## straw-its2-D05-5      0      2      0      0      0      0      0      0     27
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0     15      0      0      0      0    110      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0     49      6      0      0      0      0      0      0
## straw-its2-D12-2      0      0     33      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0    113      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      3      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      1      0      0      0      0      0      0
## straw-its2-D14-3      0      0      1      0      0      0      0      0      0
## straw-its2-D14-4      0      0      1      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      1      0      0      0      0      0      0
##                  Seq246 Seq247 Seq248 Seq249 Seq250 Seq251 Seq252 Seq253 Seq254
## straw-its2-D01-1      0      0      0     12      0      0      0      0      0
## straw-its2-D01-2      0      0      0     49      0      0      0      0      0
## straw-its2-D01-3      6      0      0     44      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      1      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0    106      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0    109      0      0      0      0      0    102      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0     16
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      2
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0    108      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0     20
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      5      0      0      0      0      0      0      0     43
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      3      0      0      0      0      0      0      0      0
## straw-its2-D14-1     65      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0    104      0      0     20
## straw-its2-D14-4      1      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1     29      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0    103      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq255 Seq256 Seq257 Seq258 Seq259 Seq260 Seq261 Seq262 Seq263
## straw-its2-D01-1      0     56      0      0      0      0      0     72     49
## straw-its2-D01-2      0     24      0      0      0      0      0      1      4
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      9      0      0      0      0      0      7     19
## straw-its2-D03-2      0      0      0      0      0      0      0      2      0
## straw-its2-D03-3      0      4      0      0      0      0      0      4      1
## straw-its2-D05-1      0      1      0      0      0      0      0      0      1
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      3      0      0      0     95      0      4      2
## straw-its2-D05-4      0      1      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      3      2
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0     85      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      1      0      0      0      0      0      0      1
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      9      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1    101      0      0      0      0      0      0      0      8
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0     99      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      1      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      3
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      2
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0     98      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0     98      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq264 Seq265 Seq266 Seq267 Seq268 Seq269 Seq270 Seq271 Seq272
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0     87      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0     84      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2     91      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0     87      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      1
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0     17
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0     28
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0     85      0      0      0
## straw-its2-D14-2      0     90      0      0      0      0      0      0      6
## straw-its2-D14-3      0      0      0      0     87      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      7
## straw-its2-D14-5      0      0      0      0      0      0      0      0     21
## straw-its2-D15-1      0      0      0      0      0      0     85      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      4
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq273 Seq274 Seq275 Seq276 Seq277 Seq278 Seq279 Seq280 Seq281
## straw-its2-D01-1      0      0      0      2     79      0      0     76     13
## straw-its2-D01-2      0      0      0     51      0      0      0      0      0
## straw-its2-D01-3      0      0      0     26      0      0      0      0      0
## straw-its2-D03-1     40      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3     23      0      0      0      0     79      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0     13
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0     79      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      2      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0     10      0      0      0      0      0      0
## straw-its2-D10-2      0      0     12      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0     35      0      0      0      0      0      0
## straw-its2-D10-5      0      0      2      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      9
## straw-its2-D12-2      0      0      3      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1     19      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      6      0      0      0      0      0      0
## straw-its2-D13-3      0      0      3      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0     10
## straw-its2-D14-2      0      0      1      0      0      0      0      0     12
## straw-its2-D14-3      0      0      0      0      0      0      0      0     15
## straw-its2-D14-4      0     83      3      0      0      0      0      0      0
## straw-its2-D14-5      0      0      3      0      0      0      0      0      3
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      1      0      0      0      0      0      0
## straw-its2-D15-3      1      0      0      0      0      0      0      0      0
##                  Seq282 Seq283 Seq284 Seq285 Seq286 Seq287 Seq288 Seq289 Seq290
## straw-its2-D01-1     52      0      0      0      0      0      0      0      0
## straw-its2-D01-2      1      0      0      0      0      0      0      0      0
## straw-its2-D01-3      1      0      0      0      0      0      0      0      0
## straw-its2-D03-1     10      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      1      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      1      0      0      0      0      0      0      0      0
## straw-its2-D05-4      5     74      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0     74      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      6      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0     20      0      0      0     73      0
## straw-its2-D12-2      0      0      0     18      0      0      0      0      0
## straw-its2-D12-3      0      0      0      3      0      0      0      0     71
## straw-its2-D13-1      1      0      0      2      0      0      0      0      0
## straw-its2-D13-2      0      0      0      9      0      0      0      0      0
## straw-its2-D13-3      0      0      0      1      0      0      0      0      0
## straw-its2-D14-1      0      0      0      1      0      0      0      0      0
## straw-its2-D14-2      0      0      0      9     74      0      0      0      0
## straw-its2-D14-3      1      0      0      1      0      0      0      0      0
## straw-its2-D14-4      0      0      0      2      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0     74      0      0      0
## straw-its2-D15-1      1      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      2      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0     74      0      0
##                  Seq291 Seq292 Seq293 Seq294 Seq295 Seq296 Seq297 Seq298 Seq299
## straw-its2-D01-1      0      0      0      0     67      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0     69      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0     65      0      0      0
## straw-its2-D05-3      0      0     69      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0     64
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0     68      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0     65      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      2      0
## straw-its2-D10-3     70      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      7      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      7      0
## straw-its2-D13-3      0      0      0      0      0      0      0      3      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      7      0
## straw-its2-D14-3      0      0      0      0      0      0      0      2      0
## straw-its2-D14-4      0      0      0      0      0      0      0     11      0
## straw-its2-D14-5      0      0      0      0      0      0      0      7      0
## straw-its2-D15-1      0      0      0      0      0      0      0     16      0
## straw-its2-D15-2      0      0      0      0      0      0      0      1      0
## straw-its2-D15-3      0      0      0      0      0      0      0      2      0
##                  Seq300 Seq301 Seq302 Seq303 Seq304 Seq305 Seq306 Seq307 Seq308
## straw-its2-D01-1      0      0      0     48      0      0     52     24      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0     38      8      0      0      5      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      1      0      0      0      3     34
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0     19     20
## straw-its2-D05-4      0      0      0      2      0      0      3      0      0
## straw-its2-D05-5      0      0      0      3      0      0      0      0      6
## straw-its2-D07-1      0      0      0      0      0     61      0      2      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3     64      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      2      0
## straw-its2-D08-3      0      0      9      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      1      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0     16      0      0      0      0      2      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      3      0
## straw-its2-D13-1      0      0      0      0      0      0      0      3      0
## straw-its2-D13-2      0      0      0      0      0      0      0      1      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0     62      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0     64      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq309 Seq310 Seq311 Seq312 Seq313 Seq314 Seq315 Seq316 Seq317
## straw-its2-D01-1      0     33      0      0     38      2      0      0      0
## straw-its2-D01-2      0      0      0      0      6     42     43      0      0
## straw-its2-D01-3      0      0      0      0      0     14     15      0      0
## straw-its2-D03-1      0      2      5      0      2      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      3      0      0      0      0
## straw-its2-D05-1      4      1      3      0      5      0      0      0      0
## straw-its2-D05-2      0      4     19      0      1      0      0      0      0
## straw-its2-D05-3      0      2     11      0      0      0      0      0      0
## straw-its2-D05-4      0     12     18      0      0      0      0      0      0
## straw-its2-D05-5      0      1      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      8      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0     59      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0     58      0
## straw-its2-D08-2      5      0      0      0      0      0      0      0     13
## straw-its2-D08-3      0      0      1      0      0      0      0      0      0
## straw-its2-D10-1      0      0      1      0      0      0      0      0      3
## straw-its2-D10-2     10      0      1      0      0      0      0      0      5
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      2      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1     21      0      0      0      0      0      0      0      8
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      5      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      6
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      2      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      8
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      7      2      0      0      1      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0     11
## straw-its2-D15-3      0      0      0      0      0      0      0      0      4
##                  Seq318 Seq319 Seq320 Seq321 Seq322 Seq323 Seq324 Seq325 Seq326
## straw-its2-D01-1      0      0     56      0     54      0      0      0     52
## straw-its2-D01-2      0      0      0     55      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0     54      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2     58      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0     53      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0     54      0      0
## straw-its2-D14-3      0     58      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq327 Seq328 Seq329 Seq330 Seq331 Seq332 Seq333 Seq334 Seq335
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0     50      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0     51      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0     51      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0     51      0     50
## straw-its2-D07-3     52      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0     52      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0     52      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0     52      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq336 Seq337 Seq338 Seq339 Seq340 Seq341 Seq342 Seq343 Seq344
## straw-its2-D01-1      0     11      0      0      0     48      0      0      0
## straw-its2-D01-2      0     29      0      0      0      0      0      0     47
## straw-its2-D01-3      0      4      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      4     45      0      0      0      0      0      0
## straw-its2-D03-3      0      1      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      7      0      0      0      0      0
## straw-its2-D05-3      0      0      0     10      0      0      0      0      0
## straw-its2-D05-4      0      0      0      8      0      0      0      0      0
## straw-its2-D05-5      0      0      0     15      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0     48      0      0
## straw-its2-D07-3      0      0      0      0     49      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      1      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5     50      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      1      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0     48      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      5      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      4      2      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq345 Seq346 Seq347 Seq348 Seq349 Seq350 Seq351 Seq352 Seq353
## straw-its2-D01-1      0      0      0      0      0     45      3      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      3      0      0
## straw-its2-D03-1      0      0      0      0      0      0      2      0     44
## straw-its2-D03-2      0      0      0      0      0      0      1      0      0
## straw-its2-D03-3      1      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      2      0      0      0      0      0      0      0
## straw-its2-D05-2      2      2      0      0      0      0      0     45      0
## straw-its2-D05-3     14      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0     10      0      0      0      0      0      0      0
## straw-its2-D05-5     12      2      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0     46      0      0      0      0      0
## straw-its2-D08-1      0      3      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      1      0      0
## straw-its2-D08-3      0      1      0      0      0      0      0      0      0
## straw-its2-D10-1      0     16      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      3      0      0
## straw-its2-D10-3      0      4     47      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      2      0      0
## straw-its2-D10-5      0      1      0      0      0      0      0      0      0
## straw-its2-D12-1      3      0      0      0      0      0      5      0      0
## straw-its2-D12-2      0      0      0      0      0      0      3      0      0
## straw-its2-D12-3      1      4      0      0      0      0      0      0      0
## straw-its2-D13-1      0      1      0      0      0      0      3      0      0
## straw-its2-D13-2      0      1      0      0     46      0      0      0      0
## straw-its2-D13-3      1      0      0      0      0      0      6      0      0
## straw-its2-D14-1      0      0      0      0      0      0      7      0      0
## straw-its2-D14-2      0      0      0      0      0      0      3      0      0
## straw-its2-D14-3      2      0      0      0      0      0      1      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      1      0      0
## straw-its2-D15-1     11      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      1      0      0
##                  Seq354 Seq355 Seq356 Seq357 Seq358 Seq359 Seq360 Seq361 Seq362
## straw-its2-D01-1      0      0      0      0     37     19      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      2     11      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0     12      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3     44      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0     44      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0     42      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0     18     43      0      0      0      1      0
## straw-its2-D10-2      0      0      0      0      0      0      0      5      0
## straw-its2-D10-3      0      0      1      0      0      0      0      0      0
## straw-its2-D10-4      0      0      3      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      6      0      0      0      0     13      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      2      0      0      0      0      1      0
## straw-its2-D13-1      0      0      3      0      3      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      3      0
## straw-its2-D13-3      0      0      7      0      0      0      0      2      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0     12      0
## straw-its2-D14-3      0      0      2      0      0      0      0      2      0
## straw-its2-D14-4      0      0      1      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0     42
## straw-its2-D15-1      0      0      1      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      3      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq363 Seq364 Seq365 Seq366 Seq367 Seq368 Seq369 Seq370 Seq371
## straw-its2-D01-1      2     40      0      0      0     35      0      0      0
## straw-its2-D01-2     30      0      0      0      0      0      0      0      0
## straw-its2-D01-3      8      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      2      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      4      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0     40      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      1      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      2      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0     40      0     39      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      3      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      2      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0     39      0
## straw-its2-D15-1      0      0     30      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0     39
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq372 Seq373 Seq374 Seq375 Seq376 Seq377 Seq378 Seq379 Seq380
## straw-its2-D01-1      0      0      0      0      0      0     36      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      2      0      0      0      0      0      0
## straw-its2-D03-1      1      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0     37      0      0      0      0      0
## straw-its2-D03-3      1      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      3      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0     37      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0     36      0
## straw-its2-D08-1      6      0      0      0      0      0      0      0      0
## straw-its2-D08-2      3      0      0      0      0      0      0      0      0
## straw-its2-D08-3      3      0      0      0      0      0      0      0      0
## straw-its2-D10-1      9      0      1      0      0      0      0      0      0
## straw-its2-D10-2      1      0      2      0      0      0      0      0      0
## straw-its2-D10-3      5      0      4      0      0      0      0      0     35
## straw-its2-D10-4      0      0      2      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      3      0      3      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      2      0      0      0      0      0      0
## straw-its2-D13-1      0      0      3      0      0      0      0      0      0
## straw-its2-D13-2      0     38      0      0      0     37      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      3      0      0      0      0      0      0
## straw-its2-D14-4      3      0      4      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0     11      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq381 Seq382 Seq383 Seq384 Seq385 Seq386 Seq387 Seq388 Seq389
## straw-its2-D01-1      0      0     12      2      0      0      0      0      0
## straw-its2-D01-2      0      0      5     16     33      0      0      0      0
## straw-its2-D01-3      0      0      0     15      0      0      0      0      0
## straw-its2-D03-1      0      0      3      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0     18      0      0      0
## straw-its2-D05-3      0      0      1      0      0      6      0      0      0
## straw-its2-D05-4      0      0      2      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      1      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0     33      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0     34      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      1      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      2      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      1      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0     33      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      1      0      0      0      0      0      0
## straw-its2-D14-1      0      0      1      0      0      0      0      0      0
## straw-its2-D14-2      0      0      2      0      0      0      0      0      0
## straw-its2-D14-3     35      0      2      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0     33
## straw-its2-D15-1      0      0      0      0      0      6      0      0      0
## straw-its2-D15-2      0      0      1      0      0      1      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq390 Seq391 Seq392 Seq393 Seq394 Seq395 Seq396 Seq397 Seq398
## straw-its2-D01-1     32      2      0      0      0      0      0      0      0
## straw-its2-D01-2      0     23      0      0      0      0      0      0      0
## straw-its2-D01-3      0      5      0      0      0      0      0      1      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0     32      0      0      0      0      0      3
## straw-its2-D05-1      0      0      0      0      0      0      0      0      1
## straw-its2-D05-2      0      0      0     32      0      0      0      0      2
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0     20
## straw-its2-D05-5      0      0      0      0      0      0      0      0      4
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      9      0      0      0      0
## straw-its2-D10-2      0      0      0      0      8      0      0      0      0
## straw-its2-D10-3      0      0      0      0      1      0      0      0      0
## straw-its2-D10-4      0      0      0      0      7      0      0      0      0
## straw-its2-D10-5      0      0      0      0      1      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      9     20      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      3      0
## straw-its2-D13-2      0      0      0      0      0      5      8      0      0
## straw-its2-D13-3      0      0      0      0      0      0      1      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      2      0
## straw-its2-D14-2      0      0      0      0      0      5     11      0      0
## straw-its2-D14-3      0      0      0      0      3      0      0      2      0
## straw-its2-D14-4      0      0      0      0      0      0      0      1      0
## straw-its2-D14-5      0      2      0      0      1      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      2      0      0
## straw-its2-D15-2      0      0      0      0      2     11      0      1      0
## straw-its2-D15-3      0      0      0      0      0     11      0      0      0
##                  Seq399 Seq400 Seq401 Seq402 Seq403 Seq404 Seq405 Seq406 Seq407
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1     30      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0     29      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0     29
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0     30     30      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0     30     30      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0     30      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0     30      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq408 Seq409 Seq410 Seq411 Seq412 Seq413 Seq414 Seq415 Seq416
## straw-its2-D01-1      8      0      0      0      0     21      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      2      0      0      0
## straw-its2-D03-2      0      3      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0     28      0      0      0      0      0      0
## straw-its2-D05-1      0      2      0      0      0      0      0      0      0
## straw-its2-D05-2      0     10      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0     27      0      0
## straw-its2-D05-4      0     12      0      0      0      2      0      0      0
## straw-its2-D05-5      1      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      1      0      0      0      0      0      0     27      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0     27
## straw-its2-D08-1      5      0      0      0      0      0      0      0      0
## straw-its2-D08-2      4      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      1      0      0      0      0      0      0      0
## straw-its2-D10-2      1      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      3      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0     28      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      1      0      0      0
## straw-its2-D13-3      5      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      1      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0     28      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq417 Seq418 Seq419 Seq420 Seq421 Seq422 Seq423 Seq424 Seq425
## straw-its2-D01-1      0      0      0      0      0     22      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0     25     21      0
## straw-its2-D01-3      0      0      0      0      0      0      0      4      0
## straw-its2-D03-1      0      0      0      0      0      3      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      3
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3     27      0      0      0     26      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0     10
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0     11
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0     16      6     27      0      0      0      0      0
## straw-its2-D12-2      0      1      0      0      0      0      0      0      1
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      2      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      3      0      0      0      0      0      0
## straw-its2-D14-2      0      3      3      0      0      0      0      0      0
## straw-its2-D14-3      0      6      0      0      0      0      0      0      0
## straw-its2-D14-4      0      1      5      0      0      0      0      0      0
## straw-its2-D14-5      0      0      8      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq426 Seq427 Seq428 Seq429 Seq430 Seq431 Seq432 Seq433 Seq434
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      1      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      2      0      0      0
## straw-its2-D03-3      0      0      0      0      0      7      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      9      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0     23      0      0
## straw-its2-D05-4      0      0      0      0      1      0      0     23      0
## straw-its2-D05-5      0      0      0      0      0      5      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3     25      0      0      0      0      0      0      0     23
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      3      0      0      0      0
## straw-its2-D08-3      0      0      0      0     11      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      1      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0     25      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      3      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      1      0      0      0      0
## straw-its2-D13-2      0      0      0     17      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      1      0      0      0      0
## straw-its2-D14-2      0      0      0      1      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      1      0      0      0      0
## straw-its2-D14-5      0      0      0      3      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0     25      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      3      0      0      0      0
##                  Seq435 Seq436 Seq437 Seq438 Seq439 Seq440 Seq441 Seq442 Seq443
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0     22      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      1      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      1      1      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      9      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      1      0      0
## straw-its2-D05-5      0      0      0      0      0      1      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3     23      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      8      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      1      0      0      0      0      7      0      0
## straw-its2-D10-2      0      9      0      0      0      2      3      6      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0     23      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      8
## straw-its2-D12-2      0      0      0      0      0      2      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      5
## straw-its2-D13-1      0      9      0      0      0      1      0      2      0
## straw-its2-D13-2      0      0      0      0      0      2      0      6      2
## straw-its2-D13-3      0      0      0      0      0      0      0      0      1
## straw-its2-D14-1      0      0      0      0      0      0      0      1      0
## straw-its2-D14-2      0      0      0      0      0      3      0      4      0
## straw-its2-D14-3      0      0      0      0      0      0      0      1      0
## straw-its2-D14-4      0      0      0     23      0      0      0      0      0
## straw-its2-D14-5      0      4      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      2      6
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      2      0      0
##                  Seq444 Seq445 Seq446 Seq447 Seq448 Seq449 Seq450 Seq451 Seq452
## straw-its2-D01-1      0      0     21     15     15     21      1      0      0
## straw-its2-D01-2      0      0      0      0      1      0      0     21      0
## straw-its2-D01-3      0      0      0      0      0      0      2      0      4
## straw-its2-D03-1      0      0      0      2      3      0      2      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      4      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      1      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      3
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      3
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      1      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      4      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      3      0      0
## straw-its2-D13-1      0      0      0      0      0      0      3      0     11
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      3      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1     22     22      0      0      0      0      1      0      0
## straw-its2-D15-2      0      0      0      0      2      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq453 Seq454 Seq455 Seq456 Seq457 Seq458 Seq459 Seq460 Seq461
## straw-its2-D01-1      0      0      0      1      0      0      0      0     19
## straw-its2-D01-2      0      0      0     11      0      0      0      0      0
## straw-its2-D01-3      0      0      0      6      1      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      6      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      2      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2     21      0      0      0      1      0      0      0      0
## straw-its2-D05-3      0      7      0      0      0      6      0      0      0
## straw-its2-D05-4      0      0      0      0      0      2      0      0      0
## straw-its2-D05-5      0      0      0      0      0      4      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0     20      0      0
## straw-its2-D07-3      0      0     21      0      0      0      0      0      0
## straw-its2-D08-1      0      5      0      0      5      0      0      0      0
## straw-its2-D08-2      0      0      0      0      4      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      1      0      0      0      0
## straw-its2-D10-2      0      5      0      0      1      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      1      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      4      0      0      0      0
## straw-its2-D14-1      0      4      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      1      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      1      0      0      0      0
## straw-its2-D15-2      0      0      0      2      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0     20      0
##                  Seq462 Seq463 Seq464 Seq465 Seq466 Seq467 Seq468 Seq469 Seq470
## straw-its2-D01-1      0      0      0      0      0     18      0      0      0
## straw-its2-D01-2     16      0      0      0      0      0      0      0      0
## straw-its2-D01-3      3      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      1      1      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      6
## straw-its2-D05-2      0      0      0      0      0      0      0      3      0
## straw-its2-D05-3      0      0      0      0      0      0      0      7      0
## straw-its2-D05-4      0      0      0      0      0      0      0      4     10
## straw-its2-D05-5      0      0      0      0      0      0      0      0      2
## straw-its2-D07-1      0     19      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0     19      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      7      0      0      0      2      0
## straw-its2-D10-2      0      0      0      0      0      0      0      1      0
## straw-its2-D10-3      0      0      0      2      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      3      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0     19      0      3      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      8      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      2      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      6      0      0
## straw-its2-D14-2      0      0      0      0      0      0      2      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      3      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq471 Seq472 Seq473 Seq474 Seq475 Seq476 Seq477 Seq478 Seq479
## straw-its2-D01-1      0      0      0     17     17     11      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0     17     17     10
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      1      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      4      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      4      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      2      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0     18      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      7      0      0      2      0      0      0
## straw-its2-D10-2      1      0      3      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      3      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      1      0      0      0
## straw-its2-D13-1      1      0      0      0      0      0      0      0      0
## straw-its2-D13-2      1      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      5      0      0      0      0      0      0
## straw-its2-D15-1      6      0      0      0      0      0      0      0      7
## straw-its2-D15-2      1      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq480 Seq481 Seq482 Seq483 Seq484 Seq485 Seq486 Seq487 Seq488
## straw-its2-D01-1      0      0      0      0      0      0      0      0     16
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      1      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      4      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      2      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0     17      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0     17      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      6      2      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      3      0      0      0      0      0      0      0
## straw-its2-D10-2      1      0      0      0      5      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0     17      0      0      0
## straw-its2-D10-4      0      4      0      0      0      0     17     17      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      4      0      0      0      0
## straw-its2-D12-2      2      0      0      0      4      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      3      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      5      0      0      0      1      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      1      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      2      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      1      0      0      0      0      0      0      0      0
##                  Seq489 Seq490 Seq491 Seq492 Seq493 Seq494 Seq495 Seq496 Seq497
## straw-its2-D01-1     16     16     16      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0     16      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      4      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      5     16      0      0      0
## straw-its2-D05-3      0      0      0      0      5      0     11     16      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      5      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0     16
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      1      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      1      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq498 Seq499 Seq500 Seq501 Seq502 Seq503 Seq504 Seq505 Seq506
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      5      0      0      0      0      0
## straw-its2-D01-3      0      0      0     10      2      0      0      0      0
## straw-its2-D03-1      0      0      0      0      1      2      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      1      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0     15      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0     15      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0     15
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      6      0      0      0
## straw-its2-D08-2      0      0      0      0      1      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      6      0      0      0      0      4      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      2      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      3      0      0      0      2      0      0      0      0
## straw-its2-D14-2      2      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0     16      0      0      0      0      0      0      0
## straw-its2-D15-2      5      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0     16      0      9      0      0      0      0
##                  Seq507 Seq508 Seq509 Seq510 Seq511 Seq512 Seq513 Seq514 Seq515
## straw-its2-D01-1      0      0      0      0      0     14      9     14     14
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      5      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2     15      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0     15      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      4      5      0      0      0      0      0
## straw-its2-D10-2      0      0      8      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      1      3      4      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      1      6      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      3      0      0      0      0
## straw-its2-D12-3      0      0      1      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      8      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      1      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq516 Seq517 Seq518 Seq519 Seq520 Seq521 Seq522 Seq523 Seq524
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2     14      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0     14      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      1      0      0      0      0      0      0
## straw-its2-D05-2      0      0      3      1      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      3      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      3      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      3     14      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0     14      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      7      0      0
## straw-its2-D08-2      0      0      0      1      0      0      0      3      1
## straw-its2-D08-3      0      0      0      1      0      0      0      0      0
## straw-its2-D10-1      0      0      2      0      0      0      0      2      0
## straw-its2-D10-2      0      0      1      2      0      0      0      0      0
## straw-its2-D10-3      0      0      2      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      3      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      1      0      0      0      1      0      5
## straw-its2-D12-2      0      0      0      0      0      0      0      0      3
## straw-its2-D12-3      0      0      1      0      0      0      1      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      1      4
## straw-its2-D13-3      0      0      0      0      0      0      0      1      0
## straw-its2-D14-1      0      0      0      0      0      0      5      0      1
## straw-its2-D14-2      0      0      0      0      0      0      0      1      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      1      0      0      0      0      0
## straw-its2-D14-5      0      0      0      2      0      0      0      3      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq525 Seq526 Seq527 Seq528 Seq529 Seq530 Seq531 Seq532 Seq533
## straw-its2-D01-1      0      0      0      0      0     13      3      0      0
## straw-its2-D01-2      0      0      0      0      0      0      8      0      0
## straw-its2-D01-3      0      0      0      0      0      0      2      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      3      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0     13
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      3      0
## straw-its2-D05-4      0      0      0      0      0      0      0      7      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      4      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4     10      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      4      3      0      0      0      0      0      0
## straw-its2-D12-2      0      1      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      2      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0     14      0      0      0      0      0
## straw-its2-D14-1      0      2      2      0      0      0      0      0      0
## straw-its2-D14-2      0      5      5      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      1      0     14      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      1      0      0      0      0      0      0
## straw-its2-D15-3      0      0      2      0      0      0      0      0      0
##                  Seq534 Seq535 Seq536 Seq537 Seq538 Seq539 Seq540 Seq541 Seq542
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2     13      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0     13      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0     13     13     13      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0     13      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      3      0      0
## straw-its2-D10-2      0      0      0      0      0      0      6      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      3      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      2      1      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      4      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      2      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      5      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0     13
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq543 Seq544 Seq545 Seq546 Seq547 Seq548 Seq549 Seq550 Seq551
## straw-its2-D01-1     12     12     12      5      7      3     12      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0     12     12
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      2      4      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      1      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      3      4      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      2      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      2      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      3      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq552 Seq553 Seq554 Seq555 Seq556 Seq557 Seq558 Seq559 Seq560
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2     12      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      6      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      8      0      0      0      0      0      0
## straw-its2-D03-3      0      1      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      4      4      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0     12     12      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0     12      0      0      0
## straw-its2-D05-5      0      1      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      2     12      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0     12
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      1      0      0
## straw-its2-D10-4      0      0      0      0      0      0      1      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      1      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      3      0      0
## straw-its2-D14-3      0      0      0      0      0      0      2      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      1      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      1      0      0
##                  Seq561 Seq562 Seq563 Seq564 Seq565 Seq566 Seq567 Seq568 Seq569
## straw-its2-D01-1      0      0      0     11     11     11      4      4      0
## straw-its2-D01-2      0      0      0      0      0      0      2      0      8
## straw-its2-D01-3      0      0      0      0      0      0      0      0      3
## straw-its2-D03-1      0      0      0      0      0      0      0      7      0
## straw-its2-D03-2      0      0      0      0      0      0      3      0      0
## straw-its2-D03-3      0      0      0      0      0      0      2      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      4      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      2      0      0      0      0      0      0      0
## straw-its2-D10-3      4      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      1      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      2      9      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      1      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      1      3      0      0      0      0      0      0
## straw-its2-D13-3      0      0      1      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      1      0      0      0      0      0      0
## straw-its2-D14-4      1      0      6      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq570 Seq571 Seq572 Seq573 Seq574 Seq575 Seq576 Seq577 Seq578
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      8     11      3      0      0      0      0      0      0
## straw-its2-D03-1      3      0      0      2      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      1      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      4      2      0      0      0      0      0
## straw-its2-D05-2      0      0      2      0      0      8      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      3      0      0      0
## straw-its2-D05-5      0      0      1      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0     11      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      3      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      1      0      0      0      2      1
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      1      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      4      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      1      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      3      2
## straw-its2-D14-1      0      0      0      0      0      0      0      5      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      4
## straw-its2-D14-3      0      0      0      2      0      0      0      1      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      2
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      1      0      5      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      2
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq579 Seq580 Seq581 Seq582 Seq583 Seq584 Seq585 Seq586 Seq587
## straw-its2-D01-1      0      0      0      0     10     10     10      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0     10     10
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      4      6     11      0      0      0      0      0      0
## straw-its2-D12-2      3      0      0      0      0      0      0      0      0
## straw-its2-D12-3      3      2      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      1      0      0      0      0      0      0      0
## straw-its2-D14-5      1      2      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0     11      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq588 Seq589 Seq590 Seq591 Seq592 Seq593 Seq594 Seq595 Seq596
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1     10      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      1      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      2      2      0      0      0      0      0      0
## straw-its2-D05-3      0      4      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      3      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0     10     10      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0     10      0      0      0
## straw-its2-D08-1      0      0      1      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      6      0      0      0      2      0      0
## straw-its2-D10-2      0      0      1      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0     10      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      3
## straw-its2-D12-2      0      0      0      0      0      0      0      0      1
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      4      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      3
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      2
## straw-its2-D14-5      0      0      0      0      0      0      0      0      1
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      4      0      0
##                  Seq597 Seq598 Seq599 Seq600 Seq601 Seq602 Seq603 Seq604 Seq605
## straw-its2-D01-1      0      9      9      1      0      0      0      0      0
## straw-its2-D01-2      0      0      0      2      6      9      9      9      0
## straw-its2-D01-3      0      0      0      3      3      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      1
## straw-its2-D03-3      0      0      0      1      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      2
## straw-its2-D05-3      0      0      0      2      0      0      0      0      2
## straw-its2-D05-4      0      0      0      0      0      0      0      0      2
## straw-its2-D05-5      0      0      0      0      0      0      0      0      2
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1     10      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq606 Seq607 Seq608 Seq609 Seq610 Seq611 Seq612 Seq613 Seq614
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      5      5      3      0      0      0      0      0      0
## straw-its2-D05-3      0      3      0      0      0      0      0      0      0
## straw-its2-D05-4      0      1      4      0      0      0      0      0      0
## straw-its2-D05-5      0      0      2      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      3      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      9      1      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      9      9      2
## straw-its2-D13-2      0      0      0      5      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      1
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      2      0      0      4
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      4      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      3      0      0      2
## straw-its2-D15-1      0      0      0      0      0      3      0      0      0
## straw-its2-D15-2      0      0      0      1      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq615 Seq616 Seq617 Seq618 Seq619 Seq620 Seq621 Seq622 Seq623
## straw-its2-D01-1      0      8      8      8      8      6      8      8      8
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      2      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      9      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq624 Seq625 Seq626 Seq627 Seq628 Seq629 Seq630 Seq631 Seq632
## straw-its2-D01-1      8      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      8      8      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      3      0      0      0      0      0
## straw-its2-D03-2      0      0      0      5      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      8      8      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      8      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      8      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      8
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq633 Seq634 Seq635 Seq636 Seq637 Seq638 Seq639 Seq640 Seq641
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      4      1      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      6      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      4      0      0      0      0      0
## straw-its2-D10-2      0      2      0      2      4      8      8      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      2      0      0      0      4      0      0      8      5
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      2      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      1      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      2      5      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      3
## straw-its2-D15-3      0      0      0      1      0      0      0      0      0
##                  Seq642 Seq643 Seq644 Seq645 Seq646 Seq647 Seq648 Seq649 Seq650
## straw-its2-D01-1      0      0      0      0      7      7      7      7      7
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      8      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      8      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      3      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      2      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      3      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      8      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq651 Seq652 Seq653 Seq654 Seq655 Seq656 Seq657 Seq658 Seq659
## straw-its2-D01-1      7      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      7      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      2      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      3      7      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      7      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      3      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      4      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      4      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      7      7
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      5      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq660 Seq661 Seq662 Seq663 Seq664 Seq665 Seq666 Seq667 Seq668
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      7      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      4      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      5      7      7      2      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      2      0      0      0      4      2      0
## straw-its2-D13-2      0      0      0      0      0      5      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      7
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      3      0      0      0      0      3      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      5      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq669 Seq670 Seq671 Seq672 Seq673 Seq674 Seq675 Seq676 Seq677
## straw-its2-D01-1      0      0      0      0      0      0      6      6      6
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      7      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      7      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      7      7      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      7      7      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq678 Seq679 Seq680 Seq681 Seq682 Seq683 Seq684 Seq685 Seq686
## straw-its2-D01-1      2      6      6      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      6      6      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      3      0      0      0
## straw-its2-D03-1      4      0      0      0      0      0      6      2      6
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      1      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      2      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      1      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      3      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq687 Seq688 Seq689 Seq690 Seq691 Seq692 Seq693 Seq694 Seq695
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      6      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      6      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      2      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      6      6      3      3      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      6      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      4      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      6
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      3      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      3      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq696 Seq697 Seq698 Seq699 Seq700 Seq701 Seq702 Seq703 Seq704
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      4      0      0      0      0      0      0      0      0
## straw-its2-D10-2      2      6      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      6      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      6      6      6      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      2      6      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      3
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      4      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      3
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq705 Seq706 Seq707 Seq708 Seq709 Seq710 Seq711 Seq712 Seq713
## straw-its2-D01-1      0      0      0      0      0      5      5      5      5
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      6      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      6      6      6      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      6      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq714 Seq715 Seq716 Seq717 Seq718 Seq719 Seq720 Seq721 Seq722
## straw-its2-D01-1      5      5      5      5      5      5      5      2      5
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      3      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq723 Seq724 Seq725 Seq726 Seq727 Seq728 Seq729 Seq730 Seq731
## straw-its2-D01-1      5      5      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      5      5      5      5      5      2      0
## straw-its2-D01-3      0      0      0      0      0      0      0      3      5
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq732 Seq733 Seq734 Seq735 Seq736 Seq737 Seq738 Seq739 Seq740
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      5      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      5      5      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      5      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      5      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      5      5      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      5      5
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq741 Seq742 Seq743 Seq744 Seq745 Seq746 Seq747 Seq748 Seq749
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      5      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      5      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      5      5      5      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      5      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      5      5      5
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq750 Seq751 Seq752 Seq753 Seq754 Seq755 Seq756 Seq757 Seq758
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      2      0      0      0      0      0      0      0      0
## straw-its2-D10-5      1      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      5      3      2      0      0      0      0      0
## straw-its2-D12-2      2      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      5      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      5      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      3      0      0      5      5      0
## straw-its2-D14-3      0      0      2      0      0      0      0      0      5
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq759 Seq760 Seq761 Seq762 Seq763 Seq764 Seq765 Seq766 Seq767
## straw-its2-D01-1      0      0      4      4      4      4      4      4      4
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      5      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      5      0      0      0      0      0      0      0
##                  Seq768 Seq769 Seq770 Seq771 Seq772 Seq773 Seq774 Seq775 Seq776
## straw-its2-D01-1      4      4      4      4      4      4      4      4      4
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq777 Seq778 Seq779 Seq780 Seq781 Seq782 Seq783 Seq784 Seq785
## straw-its2-D01-1      4      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      4      4      4      4      4      4      4      4
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq786 Seq787 Seq788 Seq789 Seq790 Seq791 Seq792 Seq793 Seq794
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      4      4      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      4      4      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      4      4      4      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      4      4
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq795 Seq796 Seq797 Seq798 Seq799 Seq800 Seq801 Seq802 Seq803
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      4      4      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      4      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      4      4      4      4      2      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      4
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      2      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq804 Seq805 Seq806 Seq807 Seq808 Seq809 Seq810 Seq811 Seq812
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      4      4      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      4      4      4      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      4      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      4      4      4
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq813 Seq814 Seq815 Seq816 Seq817 Seq818 Seq819 Seq820 Seq821
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      4      4      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      4      4      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      4      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      4      4      4      4
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq822 Seq823 Seq824 Seq825 Seq826 Seq827 Seq828 Seq829 Seq830
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      2      0      0      0      0      0      0      0      0
## straw-its2-D10-2      2      4      4      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      4      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      4      4      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      4      4      4
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq831 Seq832 Seq833 Seq834 Seq835 Seq836 Seq837 Seq838 Seq839
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      4      4      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      2      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      4      4      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      4      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      4      4      4
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      2      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq840 Seq841 Seq842 Seq843 Seq844 Seq845 Seq846 Seq847 Seq848
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      4      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      4      4      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      4      4      4      4      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      4      4
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq849 Seq850 Seq851 Seq852 Seq853 Seq854 Seq855 Seq856 Seq857
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      4      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      2      4      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      4      4      4      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      4      4      4
## straw-its2-D15-2      0      2      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq858 Seq859 Seq860 Seq861 Seq862 Seq863 Seq864 Seq865 Seq866
## straw-its2-D01-1      0      0      0      3      3      3      3      3      3
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      4      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      4      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      4      0      0      0      0      0      0
##                  Seq867 Seq868 Seq869 Seq870 Seq871 Seq872 Seq873 Seq874 Seq875
## straw-its2-D01-1      3      3      3      3      3      3      3      3      3
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq876 Seq877 Seq878 Seq879 Seq880 Seq881 Seq882 Seq883 Seq884
## straw-its2-D01-1      3      3      3      3      3      3      3      3      3
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq885 Seq886 Seq887 Seq888 Seq889 Seq890 Seq891 Seq892 Seq893
## straw-its2-D01-1      3      3      3      3      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      3      3      3      3      3
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq894 Seq895 Seq896 Seq897 Seq898 Seq899 Seq900 Seq901 Seq902
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      3      3      3      3      3      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      3      3      3      3
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq903 Seq904 Seq905 Seq906 Seq907 Seq908 Seq909 Seq910 Seq911
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      3      3      3      3      3      3      3      3      3
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq912 Seq913 Seq914 Seq915 Seq916 Seq917 Seq918 Seq919 Seq920
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      3      3      3      3      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      3      3      3      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      3      3
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq921 Seq922 Seq923 Seq924 Seq925 Seq926 Seq927 Seq928 Seq929
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      3      3      3      3      3      3      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      3      3      3
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq930 Seq931 Seq932 Seq933 Seq934 Seq935 Seq936 Seq937 Seq938
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      3      3      3      3      3      3      3      3      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      3
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq939 Seq940 Seq941 Seq942 Seq943 Seq944 Seq945 Seq946 Seq947
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      3      3      3      3      3      3      3      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      3      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      3
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq948 Seq949 Seq950 Seq951 Seq952 Seq953 Seq954 Seq955 Seq956
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      3      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      3      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      3      3      3      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      3      3      3      3
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq957 Seq958 Seq959 Seq960 Seq961 Seq962 Seq963 Seq964 Seq965
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      3      3      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      3      3      3      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      3      3      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      3      3
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq966 Seq967 Seq968 Seq969 Seq970 Seq971 Seq972 Seq973 Seq974
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      3      3      3      3      3      3      3      3      3
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq975 Seq976 Seq977 Seq978 Seq979 Seq980 Seq981 Seq982 Seq983
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      3      3      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      3      3      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      3      3      3      3      3
## straw-its2-D13-1      0      0      0      0      0      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      0      0      0      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      0
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq984 Seq985 Seq986 Seq987 Seq988 Seq989 Seq990 Seq991 Seq992
## straw-its2-D01-1      0      0      0      0      0      0      0      0      0
## straw-its2-D01-2      0      0      0      0      0      0      0      0      0
## straw-its2-D01-3      0      0      0      0      0      0      0      0      0
## straw-its2-D03-1      0      0      0      0      0      0      0      0      0
## straw-its2-D03-2      0      0      0      0      0      0      0      0      0
## straw-its2-D03-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-1      0      0      0      0      0      0      0      0      0
## straw-its2-D05-2      0      0      0      0      0      0      0      0      0
## straw-its2-D05-3      0      0      0      0      0      0      0      0      0
## straw-its2-D05-4      0      0      0      0      0      0      0      0      0
## straw-its2-D05-5      0      0      0      0      0      0      0      0      0
## straw-its2-D07-1      0      0      0      0      0      0      0      0      0
## straw-its2-D07-2      0      0      0      0      0      0      0      0      0
## straw-its2-D07-3      0      0      0      0      0      0      0      0      0
## straw-its2-D08-1      0      0      0      0      0      0      0      0      0
## straw-its2-D08-2      0      0      0      0      0      0      0      0      0
## straw-its2-D08-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-1      0      0      0      0      0      0      0      0      0
## straw-its2-D10-2      0      0      0      0      0      0      0      0      0
## straw-its2-D10-3      0      0      0      0      0      0      0      0      0
## straw-its2-D10-4      0      0      0      0      0      0      0      0      0
## straw-its2-D10-5      0      0      0      0      0      0      0      0      0
## straw-its2-D12-1      0      0      0      0      0      0      0      0      0
## straw-its2-D12-2      0      0      0      0      0      0      0      0      0
## straw-its2-D12-3      0      0      0      0      0      0      0      0      0
## straw-its2-D13-1      3      3      3      3      3      0      0      0      0
## straw-its2-D13-2      0      0      0      0      0      3      3      3      0
## straw-its2-D13-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-1      0      0      0      0      0      0      0      0      3
## straw-its2-D14-2      0      0      0      0      0      0      0      0      0
## straw-its2-D14-3      0      0      0      0      0      0      0      0      0
## straw-its2-D14-4      0      0      0      0      0      0      0      0      0
## straw-its2-D14-5      0      0      0      0      0      0      0      0      0
## straw-its2-D15-1      0      0      0      0      0      0      0      0      0
## straw-its2-D15-2      0      0      0      0      0      0      0      0      0
## straw-its2-D15-3      0      0      0      0      0      0      0      0      0
##                  Seq993 Seq994 Seq995 Seq996 Seq997 Seq998 Seq999 Seq1000
## straw-its2-D01-1      0      0      0      0      0      0      0       0
## straw-its2-D01-2      0      0      0      0      0      0      0       0
## straw-its2-D01-3      0      0      0      0      0      0      0       0
## straw-its2-D03-1      0      0      0      0      0      0      0       0
## straw-its2-D03-2      0      0      0      0      0      0      0       0
## straw-its2-D03-3      0      0      0      0      0      0      0       0
## straw-its2-D05-1      0      0      0      0      0      0      0       0
## straw-its2-D05-2      0      0      0      0      0      0      0       0
## straw-its2-D05-3      0      0      0      0      0      0      0       0
## straw-its2-D05-4      0      0      0      0      0      0      0       0
## straw-its2-D05-5      0      0      0      0      0      0      0       0
## straw-its2-D07-1      0      0      0      0      0      0      0       0
## straw-its2-D07-2      0      0      0      0      0      0      0       0
## straw-its2-D07-3      0      0      0      0      0      0      0       0
## straw-its2-D08-1      0      0      0      0      0      0      0       0
## straw-its2-D08-2      0      0      0      0      0      0      0       0
## straw-its2-D08-3      0      0      0      0      0      0      0       0
## straw-its2-D10-1      0      0      0      0      0      0      0       0
## straw-its2-D10-2      0      0      0      0      0      0      0       0
## straw-its2-D10-3      0      0      0      0      0      0      0       0
## straw-its2-D10-4      0      0      0      0      0      0      0       0
## straw-its2-D10-5      0      0      0      0      0      0      0       0
## straw-its2-D12-1      0      0      0      0      0      0      0       0
## straw-its2-D12-2      0      0      0      0      0      0      0       0
## straw-its2-D12-3      0      0      0      0      0      0      0       0
## straw-its2-D13-1      0      0      0      0      0      0      0       0
## straw-its2-D13-2      0      0      0      0      0      0      0       0
## straw-its2-D13-3      0      0      0      0      0      0      0       0
## straw-its2-D14-1      3      3      3      3      0      0      0       0
## straw-its2-D14-2      0      0      0      0      3      3      3       3
## straw-its2-D14-3      0      0      0      0      0      0      0       0
## straw-its2-D14-4      0      0      0      0      0      0      0       0
## straw-its2-D14-5      0      0      0      0      0      0      0       0
## straw-its2-D15-1      0      0      0      0      0      0      0       0
## straw-its2-D15-2      0      0      0      0      0      0      0       0
## straw-its2-D15-3      0      0      0      0      0      0      0       0
##                  Seq1001 Seq1002 Seq1003 Seq1004 Seq1005 Seq1006 Seq1007
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       3       0       0       0       0       0       0
## straw-its2-D14-3       0       3       3       3       3       0       0
## straw-its2-D14-4       0       0       0       0       0       3       0
## straw-its2-D14-5       0       0       0       0       0       0       3
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1008 Seq1009 Seq1010 Seq1011 Seq1012 Seq1013 Seq1014
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       3       3       3       0       0       0       0
## straw-its2-D15-1       0       0       0       3       3       3       3
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1015 Seq1016 Seq1017 Seq1018 Seq1019 Seq1020 Seq1021
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       3       3       3       3       3       3       0
## straw-its2-D15-2       0       0       0       0       0       0       3
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1022 Seq1023 Seq1024 Seq1025 Seq1026 Seq1027 Seq1028
## straw-its2-D01-1       0       0       0       0       2       2       2
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       3       0       0       0       0       0       0
## straw-its2-D15-3       0       3       3       3       0       0       0
##                  Seq1029 Seq1030 Seq1031 Seq1032 Seq1033 Seq1034 Seq1035
## straw-its2-D01-1       2       2       2       2       2       2       2
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1036 Seq1037 Seq1038 Seq1039 Seq1040 Seq1041 Seq1042
## straw-its2-D01-1       2       2       2       2       2       2       2
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1043 Seq1044 Seq1045 Seq1046 Seq1047 Seq1048 Seq1049
## straw-its2-D01-1       2       2       2       2       2       2       2
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1050 Seq1051 Seq1052 Seq1053 Seq1054 Seq1055 Seq1056
## straw-its2-D01-1       2       2       2       2       2       2       2
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1057 Seq1058 Seq1059 Seq1060 Seq1061 Seq1062 Seq1063
## straw-its2-D01-1       2       2       2       2       2       2       2
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1064 Seq1065 Seq1066 Seq1067 Seq1068 Seq1069 Seq1070
## straw-its2-D01-1       2       0       0       0       0       0       0
## straw-its2-D01-2       0       2       2       2       2       2       2
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1071 Seq1072 Seq1073 Seq1074 Seq1075 Seq1076 Seq1077
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       2       2       2       2       2       2       2
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1078 Seq1079 Seq1080 Seq1081 Seq1082 Seq1083 Seq1084
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       2       2       2       2       2       2       0
## straw-its2-D01-3       0       0       0       0       0       0       2
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1085 Seq1086 Seq1087 Seq1088 Seq1089 Seq1090 Seq1091
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       2       2       2       0       0       0       0
## straw-its2-D03-1       0       0       0       2       2       2       2
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1092 Seq1093 Seq1094 Seq1095 Seq1096 Seq1097 Seq1098
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       2       2       2       2       2       2       2
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1099 Seq1100 Seq1101 Seq1102 Seq1103 Seq1104 Seq1105
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       2       2       2       2       0       0       0
## straw-its2-D03-2       0       0       0       0       2       2       2
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1106 Seq1107 Seq1108 Seq1109 Seq1110 Seq1111 Seq1112
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       2       0       0       0       0       0       0
## straw-its2-D03-3       0       2       2       2       0       0       0
## straw-its2-D05-1       0       0       0       0       2       2       2
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1113 Seq1114 Seq1115 Seq1116 Seq1117 Seq1118 Seq1119
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       2       2       0       0       0       0       0
## straw-its2-D05-2       0       0       2       2       2       2       2
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1120 Seq1121 Seq1122 Seq1123 Seq1124 Seq1125 Seq1126
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       2       2       2       2       2       2       2
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1127 Seq1128 Seq1129 Seq1130 Seq1131 Seq1132 Seq1133
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       2       2       2       0       0       0       0
## straw-its2-D05-3       0       0       0       2       2       2       2
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1134 Seq1135 Seq1136 Seq1137 Seq1138 Seq1139 Seq1140
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       2       2       2       0       0       0       0
## straw-its2-D05-4       0       0       0       2       2       2       2
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1141 Seq1142 Seq1143 Seq1144 Seq1145 Seq1146 Seq1147
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       2       2       2       2       2       2       0
## straw-its2-D05-5       0       0       0       0       0       0       2
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1148 Seq1149 Seq1150 Seq1151 Seq1152 Seq1153 Seq1154
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       2       0       0       0       0       0       0
## straw-its2-D07-1       0       2       0       0       0       0       0
## straw-its2-D07-2       0       0       2       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       2       2       0       0
## straw-its2-D08-2       0       0       0       0       0       2       2
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1155 Seq1156 Seq1157 Seq1158 Seq1159 Seq1160 Seq1161
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       2       2       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       2       2       2       2       2
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1162 Seq1163 Seq1164 Seq1165 Seq1166 Seq1167 Seq1168
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       2       2       0       0       0       0       0
## straw-its2-D10-2       0       0       2       2       2       2       2
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1169 Seq1170 Seq1171 Seq1172 Seq1173 Seq1174 Seq1175
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       2       2       2       2       2       2       2
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1176 Seq1177 Seq1178 Seq1179 Seq1180 Seq1181 Seq1182
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       2       0       0       0       0       0       0
## straw-its2-D10-3       0       2       2       0       0       0       0
## straw-its2-D10-4       0       0       0       2       2       2       2
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1183 Seq1184 Seq1185 Seq1186 Seq1187 Seq1188 Seq1189
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       2       2       2       0       0       0       0
## straw-its2-D10-5       0       0       0       2       2       2       0
## straw-its2-D12-1       0       0       0       0       0       0       2
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1190 Seq1191 Seq1192 Seq1193 Seq1194 Seq1195 Seq1196
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       2       2       2       2       2       2       2
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1197 Seq1198 Seq1199 Seq1200 Seq1201 Seq1202 Seq1203
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       2       2       2       2       2       2       2
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1204 Seq1205 Seq1206 Seq1207 Seq1208 Seq1209 Seq1210
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       2       2       2       2       0       0       0
## straw-its2-D12-2       0       0       0       0       2       2       2
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1211 Seq1212 Seq1213 Seq1214 Seq1215 Seq1216 Seq1217
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       2       2       2       0       0       0       0
## straw-its2-D12-3       0       0       0       2       2       2       2
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1218 Seq1219 Seq1220 Seq1221 Seq1222 Seq1223 Seq1224
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       2       2       2       2       2       2       2
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1225 Seq1226 Seq1227 Seq1228 Seq1229 Seq1230 Seq1231
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       2       2       0       0       0       0       0
## straw-its2-D13-2       0       0       2       2       2       2       2
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1232 Seq1233 Seq1234 Seq1235 Seq1236 Seq1237 Seq1238
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       2       2       2       2       2       2       0
## straw-its2-D13-3       0       0       0       0       0       0       2
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1239 Seq1240 Seq1241 Seq1242 Seq1243 Seq1244 Seq1245
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       2       0       0       0       0       0       0
## straw-its2-D14-1       0       2       2       2       2       2       2
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1246 Seq1247 Seq1248 Seq1249 Seq1250 Seq1251 Seq1252
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       2       0       0       0       0       0       0
## straw-its2-D14-2       0       2       2       2       2       2       2
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1253 Seq1254 Seq1255 Seq1256 Seq1257 Seq1258 Seq1259
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       2       2       2       2       2       0       0
## straw-its2-D14-3       0       0       0       0       0       2       2
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1260 Seq1261 Seq1262 Seq1263 Seq1264 Seq1265 Seq1266
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       2       2       2       0       0       0       0
## straw-its2-D14-4       0       0       0       2       2       2       2
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1267 Seq1268 Seq1269 Seq1270 Seq1271 Seq1272 Seq1273
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       2       2       0       0       0       0       0
## straw-its2-D14-5       0       0       2       2       2       2       2
## straw-its2-D15-1       0       0       0       0       0       0       0
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1274 Seq1275 Seq1276 Seq1277 Seq1278 Seq1279 Seq1280
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       2       0       0       0       0       0       0
## straw-its2-D15-1       0       2       2       2       2       2       2
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1281 Seq1282 Seq1283 Seq1284 Seq1285 Seq1286 Seq1287
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       2       2       2       2       2       2       2
## straw-its2-D15-2       0       0       0       0       0       0       0
## straw-its2-D15-3       0       0       0       0       0       0       0
##                  Seq1288 Seq1289 Seq1290 Seq1291 Seq1292 Seq1293 Seq1294
## straw-its2-D01-1       0       0       0       0       0       0       0
## straw-its2-D01-2       0       0       0       0       0       0       0
## straw-its2-D01-3       0       0       0       0       0       0       0
## straw-its2-D03-1       0       0       0       0       0       0       0
## straw-its2-D03-2       0       0       0       0       0       0       0
## straw-its2-D03-3       0       0       0       0       0       0       0
## straw-its2-D05-1       0       0       0       0       0       0       0
## straw-its2-D05-2       0       0       0       0       0       0       0
## straw-its2-D05-3       0       0       0       0       0       0       0
## straw-its2-D05-4       0       0       0       0       0       0       0
## straw-its2-D05-5       0       0       0       0       0       0       0
## straw-its2-D07-1       0       0       0       0       0       0       0
## straw-its2-D07-2       0       0       0       0       0       0       0
## straw-its2-D07-3       0       0       0       0       0       0       0
## straw-its2-D08-1       0       0       0       0       0       0       0
## straw-its2-D08-2       0       0       0       0       0       0       0
## straw-its2-D08-3       0       0       0       0       0       0       0
## straw-its2-D10-1       0       0       0       0       0       0       0
## straw-its2-D10-2       0       0       0       0       0       0       0
## straw-its2-D10-3       0       0       0       0       0       0       0
## straw-its2-D10-4       0       0       0       0       0       0       0
## straw-its2-D10-5       0       0       0       0       0       0       0
## straw-its2-D12-1       0       0       0       0       0       0       0
## straw-its2-D12-2       0       0       0       0       0       0       0
## straw-its2-D12-3       0       0       0       0       0       0       0
## straw-its2-D13-1       0       0       0       0       0       0       0
## straw-its2-D13-2       0       0       0       0       0       0       0
## straw-its2-D13-3       0       0       0       0       0       0       0
## straw-its2-D14-1       0       0       0       0       0       0       0
## straw-its2-D14-2       0       0       0       0       0       0       0
## straw-its2-D14-3       0       0       0       0       0       0       0
## straw-its2-D14-4       0       0       0       0       0       0       0
## straw-its2-D14-5       0       0       0       0       0       0       0
## straw-its2-D15-1       2       2       2       2       0       0       0
## straw-its2-D15-2       0       0       0       0       2       2       0
## straw-its2-D15-3       0       0       0       0       0       0       2
##                  Seq1295
## straw-its2-D01-1       0
## straw-its2-D01-2       0
## straw-its2-D01-3       0
## straw-its2-D03-1       0
## straw-its2-D03-2       0
## straw-its2-D03-3       0
## straw-its2-D05-1       0
## straw-its2-D05-2       0
## straw-its2-D05-3       0
## straw-its2-D05-4       0
## straw-its2-D05-5       0
## straw-its2-D07-1       0
## straw-its2-D07-2       0
## straw-its2-D07-3       0
## straw-its2-D08-1       0
## straw-its2-D08-2       0
## straw-its2-D08-3       0
## straw-its2-D10-1       0
## straw-its2-D10-2       0
## straw-its2-D10-3       0
## straw-its2-D10-4       0
## straw-its2-D10-5       0
## straw-its2-D12-1       0
## straw-its2-D12-2       0
## straw-its2-D12-3       0
## straw-its2-D13-1       0
## straw-its2-D13-2       0
## straw-its2-D13-3       0
## straw-its2-D14-1       0
## straw-its2-D14-2       0
## straw-its2-D14-3       0
## straw-its2-D14-4       0
## straw-its2-D14-5       0
## straw-its2-D15-1       0
## straw-its2-D15-2       0
## straw-its2-D15-3       2

Все its филотипы

amp_its

## ampvis2 object with 4 elements. 
## Summary of OTU table:
##      Samples         OTUs  Total#Reads    Min#Reads    Max#Reads Median#Reads 
##           36         1295       815134        13533        34994        22657 
##    Avg#Reads 
##     22642.61 
## 
## Assigned taxonomy:
##     Kingdom      Phylum       Class       Order      Family       Genus 
##    554(43%) 453(34.98%) 362(27.95%) 335(25.87%) 321(24.79%) 306(23.63%) 
##     Species 
## 207(15.98%) 
## 
## Metadata variables: 3 
##  SampleID, Day, Description

Филы ао всем

amp_heatmap(amp_its,
            tax_show = 7,
            group_by = "Day",
            tax_aggregate = "Phylum",
            tax_add = "Kingdom",
            normalise=TRUE,
            showRemainingTaxa = TRUE)

## Warning: Transformation introduced infinite values in discrete y-axis

Те, которые пойдут в WGCNA(116 филотипов)

amp_heatmap(amp_its_inall,
            tax_show = 40 ,
            group_by = "Day",
            tax_aggregate = "OTU",
            tax_add = "Genus",
            normalise=TRUE,
            showRemainingTaxa = TRUE)

## Warning: Transformation introduced infinite values in discrete y-axis

beta_custom_norm_NMDS_elli_w(ps_its, Group="Day", Color="Day")

## Square root transformation
## Wisconsin double standardization
## Run 0 stress 0.1834317 
## Run 1 stress 0.2171478 
## Run 2 stress 0.1841584 
## Run 3 stress 0.1841584 
## Run 4 stress 0.1834317 
## ... Procrustes: rmse 0.00001261231  max resid 0.00003592311 
## ... Similar to previous best
## Run 5 stress 0.1834317 
## ... New best solution
## ... Procrustes: rmse 0.0000323766  max resid 0.00007990469 
## ... Similar to previous best
## Run 6 stress 0.231872 
## Run 7 stress 0.1841584 
## Run 8 stress 0.1841585 
## Run 9 stress 0.1834317 
## ... New best solution
## ... Procrustes: rmse 0.00001517606  max resid 0.00006303215 
## ... Similar to previous best
## Run 10 stress 0.1841585 
## Run 11 stress 0.1834317 
## ... Procrustes: rmse 0.000038015  max resid 0.000121633 
## ... Similar to previous best
## Run 12 stress 0.1841584 
## Run 13 stress 0.1841584 
## Run 14 stress 0.1834318 
## ... Procrustes: rmse 0.00003306352  max resid 0.000165396 
## ... Similar to previous best
## Run 15 stress 0.1834317 
## ... Procrustes: rmse 0.00004440375  max resid 0.0001204561 
## ... Similar to previous best
## Run 16 stress 0.2231292 
## Run 17 stress 0.1834317 
## ... New best solution
## ... Procrustes: rmse 0.0000110403  max resid 0.0000307291 
## ... Similar to previous best
## Run 18 stress 0.1841584 
## Run 19 stress 0.2306513 
## Run 20 stress 0.1841584 
## *** Solution reached

otu.inall <- as.data.frame(ps.its.inall@otu_table@.Data) 
ps.inall.clr <- ps.its.inall
otu_table(ps.inall.clr) <- phyloseq::otu_table(compositions::clr(otu.inall), taxa_are_rows = FALSE)
data <- ps.inall.clr@otu_table@.Data %>% 
  as.data.frame()
rownames(data) <- as.character(ps.inall.clr@sam_data$Description)
powers <-  c(c(1:10), seq(from = 12, to=30, by=1))
sft <-  pickSoftThreshold(data, powerVector = powers, verbose = 5, networkType = "signed")

## pickSoftThreshold: will use block size 116.
##  pickSoftThreshold: calculating connectivity for given powers...
##    ..working on genes 1 through 116 of 116
##    Power SFT.R.sq  slope truncated.R.sq mean.k. median.k. max.k.
## 1      1   0.3860 -25.70         0.5550 57.2000 57.100000 59.600
## 2      2   0.4580 -17.60         0.3190 29.5000 29.300000 32.400
## 3      3   0.4860 -11.60         0.3420 15.8000 15.600000 18.500
## 4      4   0.6370  -7.48         0.5340  8.7100  8.530000 11.300
## 5      5   0.7900  -5.30         0.7300  4.9900  4.780000  7.370
## 6      6   0.8050  -3.89         0.7500  2.9600  2.770000  5.090
## 7      7   0.8400  -2.94         0.8030  1.8300  1.650000  3.710
## 8      8   0.7890  -2.65         0.7530  1.1700  1.000000  2.820
## 9      9   0.2080  -7.17        -0.0165  0.7790  0.626000  2.230
## 10    10   0.8000  -1.88         0.8250  0.5400  0.401000  1.810
## 11    12   0.8610  -1.51         0.9500  0.2890  0.173000  1.360
## 12    13   0.7990  -1.44         0.8150  0.2230  0.121000  1.220
## 13    14   0.8000  -1.34         0.8260  0.1770  0.083400  1.120
## 14    15   0.7480  -1.32         0.7690  0.1440  0.057500  1.020
## 15    16   0.1540  -2.22        -0.0813  0.1190  0.040100  0.941
## 16    17   0.1530  -2.10        -0.0816  0.1010  0.027800  0.869
## 17    18   0.8570  -1.15         0.8780  0.0866  0.019400  0.805
## 18    19   0.0897  -1.46        -0.0687  0.0754  0.013700  0.746
## 19    20   0.0930  -1.43        -0.0720  0.0663  0.009910  0.693
## 20    21   0.2140  -2.01         0.0509  0.0589  0.007180  0.660
## 21    22   0.2200  -2.04         0.0648  0.0527  0.005230  0.643
## 22    23   0.0951  -1.76        -0.0800  0.0475  0.003800  0.627
## 23    24   0.1930  -1.80        -0.0248  0.0431  0.002730  0.612
## 24    25   0.1960  -1.82        -0.0191  0.0393  0.001970  0.598
## 25    26   0.2010  -2.33        -0.0126  0.0360  0.001440  0.585
## 26    27   0.2220  -2.37         0.0609  0.0331  0.001070  0.572
## 27    28   0.2290  -2.40         0.0789  0.0305  0.000788  0.559
## 28    29   0.2320  -2.67         0.0387  0.0283  0.000584  0.547
## 29    30   0.2430  -2.73         0.0567  0.0263  0.000433  0.536

plot(sft$fitIndices[,1], -sign(sft$fitIndices[,3])*sft$fitIndices[,2], xlab="Soft Threshold (power)",ylab="Scale Free Topology Model Fit,signed R^2",type="n", main = paste("Scale independence"))
text(sft$fitIndices[,1], -sign(sft$fitIndices[,3])*sft$fitIndices[,2], labels=powers,cex=0.9,col="red")
abline(h=0.9,col="salmon")

ps.varstab <- ps_vst(ps.its.inall, "Day")

data2 <- ps.varstab@otu_table@.Data %>% 
  as.data.frame()

rownames(data2) <- as.character(ps.varstab@sam_data$Description)
powers <-  c(seq(from = 1, to=10, by=0.5), seq(from = 11, to=20, by=1))
sft2 <-  pickSoftThreshold(data2, powerVector = powers, verbose = 5, networkType = "signed hybrid")

## pickSoftThreshold: will use block size 116.
##  pickSoftThreshold: calculating connectivity for given powers...
##    ..working on genes 1 through 116 of 116
##    Power SFT.R.sq  slope truncated.R.sq mean.k.  median.k. max.k.
## 1    1.0    0.352 -1.180        0.82400 11.7000 11.1000000  21.40
## 2    1.5    0.511 -0.965        0.80800  6.4500  5.9700000  13.20
## 3    2.0    0.688 -1.020        0.82800  3.9000  3.3000000   9.04
## 4    2.5    0.787 -0.915        0.80900  2.5200  1.9300000   6.41
## 5    3.0    0.828 -0.797        0.78200  1.7200  1.1700000   4.69
## 6    3.5    0.807 -0.913        0.75900  1.2300  0.7570000   3.81
## 7    4.0    0.789 -1.110        0.73200  0.9160  0.5010000   3.54
## 8    4.5    0.200 -2.790        0.08170  0.7060  0.3370000   3.34
## 9    5.0    0.218 -2.720        0.11300  0.5610  0.2300000   3.18
## 10   5.5    0.235 -3.520        0.08280  0.4570  0.1590000   3.04
## 11   6.0    0.165 -2.140       -0.07370  0.3800  0.1100000   2.92
## 12   6.5    0.204 -2.250       -0.00278  0.3220  0.0758000   2.82
## 13   7.0    0.208 -2.210        0.00364  0.2780  0.0538000   2.73
## 14   7.5    0.192 -2.760       -0.03940  0.2430  0.0391000   2.65
## 15   8.0    0.192 -2.670       -0.03910  0.2150  0.0280000   2.57
## 16   8.5    0.196 -2.600       -0.03270  0.1920  0.0203000   2.50
## 17   9.0    0.228 -2.770        0.01890  0.1740  0.0148000   2.44
## 18   9.5    0.213 -2.990       -0.00815  0.1580  0.0108000   2.37
## 19  10.0    0.214 -3.060       -0.00549  0.1450  0.0078000   2.32
## 20  11.0    0.230 -3.170        0.01010  0.1240  0.0041500   2.21
## 21  12.0    0.262 -3.210        0.05580  0.1080  0.0024100   2.12
## 22  13.0    0.222 -3.130        0.01010  0.0955  0.0014200   2.03
## 23  14.0    0.234 -3.060        0.02160  0.0855  0.0008090   1.95
## 24  15.0    0.259 -3.140        0.04770  0.0774  0.0004490   1.88
## 25  16.0    0.270 -3.070        0.06090  0.0707  0.0002500   1.82
## 26  17.0    0.233 -2.840        0.04610  0.0651  0.0001400   1.76
## 27  18.0    0.244 -2.790        0.05240  0.0602  0.0000789   1.71
## 28  19.0    0.251 -2.860        0.06040  0.0561  0.0000444   1.66
## 29  20.0    0.280 -3.030        0.08580  0.0525  0.0000250   1.62

plot(sft2$fitIndices[,1], -sign(sft2$fitIndices[,3])*sft2$fitIndices[,2], xlab="Soft Threshold (power)",ylab="Scale Free Topology Model Fit,signed R^2",type="n", main = paste("Scale independence"))
text(sft2$fitIndices[,1], -sign(sft2$fitIndices[,3])*sft2$fitIndices[,2], labels=powers,cex=0.9,col="red")
abline(h=0.9,col="salmon")

# WGCNA, as old and not supported
detachAllPackages()
library(WGCNA)


net3 <- WGCNA::blockwiseModules(data2,
                          power=3,
                          TOMType="signed",
                          networkType="signed hybrid",
                          nThreads=0)


mergedColors2 <-  WGCNA::labels2colors(net3$colors, colorSeq = c("salmon", "darkgreen", "cyan", "red", "blue", "plum"))

plotDendroAndColors(
  net3$dendrograms[[1]],
  mergedColors2[net3$blockGenes[[1]]],
  "Module colors",
  dendroLabels = FALSE,
  hang = 0.03,
  addGuide = TRUE,
  guideHang = 0.05)

library(phyloseq)
library(tidyverse)
library(ggpubr)
library(ampvis2)
library(heatmaply)
library(WGCNA)
library(phyloseq)
library(ggtree)
library(tidyverse)
library(KneeArrower)

Зеленые - фон

modules_of_interest = mergedColors2 %>% 
  unique()

module_df <- data.frame(
  asv = names(net3$colors),
  colors = mergedColors2
)

# module_df[module_df == "yellow"] <- "salmon"

submod <-  module_df %>%
  subset(colors %in% modules_of_interest)

row.names(module_df) = module_df$asv

subexpr = as.data.frame(t(data2))[submod$asv,]

submod_df <- data.frame(subexpr) %>%
  mutate(
    asv = row.names(.)
  ) %>%
  pivot_longer(-asv) %>%
  mutate(
    module = module_df[asv,]$colors
  )

submod_df <- submod_df %>% 
  mutate(name = gsub("\\_.*","",submod_df$name)) %>% 
  group_by(name, asv) %>% 
  summarise(value = mean(value), asv = asv, module = module) %>% 
  relocate(c(asv, name, value, module)) %>% 
  ungroup() %>% 
  mutate(module = as.factor(module))

p <- submod_df %>% 
  ggplot(., aes(x=name, y=value, group=asv)) +
  geom_line(aes(color = module),
            alpha = 0.2) +
  theme_bw() +
  theme(
    axis.text.x = element_text(angle = 90),
    legend.position = "none") +
  facet_grid(rows = vars(module)) +
  labs(x = "treatment",
       y = "normalized expression")

p + scale_color_manual(values = levels(submod_df$module))

l_its <- color_filt_broken(ps_its, submod_df, ps.its.inall)
l_its

$cyan \(cyan\)ps phyloseq-class experiment-level object otu_table() OTU Table: [ 36 taxa and 36 samples ] sample_data() Sample Data: [ 36 samples by 2 sample variables ] tax_table() Taxonomy Table: [ 36 taxa by 7 taxonomic ranks ] refseq() DNAStringSet: [ 36 reference sequences ]

\(cyan\)amp ampvis2 object with 4 elements. Summary of OTU table: Samples OTUs Total#Reads Min#Reads Max#Reads Median#Reads 36 36 318660 0 23584 9007.5 Avg#Reads 8851.67

Assigned taxonomy: Kingdom Phylum Class Order Family Genus Species 28(78%) 25(69.44%) 17(47.22%) 12(33.33%) 12(33.33%) 12(33.33%) 9(25%)

Metadata variables: 3 SampleID, Day, Description

\(cyan\)heat \(cyan\)heat_rel \(cyan\)tree \(cyan\)taxa

	Kingdom	Phylum	Class	Order	Family	Genus	Species
Seq1	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Chaetosphaeriales	f__Chaetosphaeriaceae	g__Chloridium	s__aseptatum
Seq2	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Coniochaetales	f__Coniochaetaceae	g__Coniochaeta	s__verticillata
Seq12	k__Fungi	p__Ascomycota	c__Sordariomycetes	NA	NA	NA	NA
Seq184	NA	NA	NA	NA	NA	NA	NA
Seq10	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Coniochaetales	f__Coniochaetaceae	g__Coniochaeta	s__canina
Seq73	k__Alveolata	NA	NA	NA	NA	NA	NA
Seq5	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Coniochaetales	f__Coniochaetaceae	g__Coniochaeta	s__verticillata
Seq118	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA
Seq18	k__Fungi	p__Ascomycota	c__Sordariomycetes	NA	NA	NA	NA
Seq93	k__Fungi	p__Ascomycota	c__Dothideomycetes	o__Venturiales	f__Sympoventuriaceae	g__Scolecobasidium	s__constrictum
Seq78	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA
Seq101	NA	NA	NA	NA	NA	NA	NA
Seq4	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Coniochaetales	f__Coniochaetaceae	g__Coniochaeta	s__verticillata
Seq62	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA
Seq48	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA
Seq36	k__Fungi	p__Ascomycota	c__Sordariomycetes	NA	NA	NA	NA
Seq44	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA
Seq103	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA
Seq166	k__Fungi	p__Ascomycota	c__Eurotiomycetes	o__Chaetothyriales	f__Herpotrichiellaceae	g__Exophiala	NA
Seq231	k__Eukaryota_kgd_Incertae_sedis	NA	NA	NA	NA	NA	NA
Seq21	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Clavicipitaceae	g__Metarhizium	s__marquandii
Seq108	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA
Seq74	k__Fungi	p__Ascomycota	c__Sordariomycetes	NA	NA	NA	NA
Seq195	NA	NA	NA	NA	NA	NA	NA
Seq28	k__Fungi	p__Ascomycota	c__Leotiomycetes	o__Helotiales	f__Helotiaceae	g__Scytalidium	NA
Seq104	k__Metazoa	p__Nematoda	c__Chromadorea	o__Rhabditida	f__Cephalobidae	g__Pseudacrobeles	NA
Seq29	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Glomerellales	f__Glomerellaceae	g__Colletotrichum	s__sidae
Seq46	k__Fungi	p__Ascomycota	c__Sordariomycetes	NA	NA	NA	NA
Seq122	NA	NA	NA	NA	NA	NA	NA
Seq235	NA	NA	NA	NA	NA	NA	NA
Seq191	NA	NA	NA	NA	NA	NA	NA
Seq94	k__Alveolata	NA	NA	NA	NA	NA	NA
Seq76	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Sordariales_fam_Incertae_sedis	g__Staphylotrichum	s__boninense
Seq155	NA	NA	NA	NA	NA	NA	NA
Seq190	NA	NA	NA	NA	NA	NA	NA
Seq32	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA

$darkgreen \(darkgreen\)ps phyloseq-class experiment-level object otu_table() OTU Table: [ 56 taxa and 36 samples ] sample_data() Sample Data: [ 36 samples by 2 sample variables ] tax_table() Taxonomy Table: [ 56 taxa by 7 taxonomic ranks ] refseq() DNAStringSet: [ 56 reference sequences ]

\(darkgreen\)amp ampvis2 object with 4 elements. Summary of OTU table: Samples OTUs Total#Reads Min#Reads Max#Reads Median#Reads 36 56 261584 1790 20315 6577 Avg#Reads 7266.22

Assigned taxonomy: Kingdom Phylum Class Order Family Genus Species 52(93%) 51(91.07%) 48(85.71%) 48(85.71%) 48(85.71%) 48(85.71%) 34(60.71%)

Metadata variables: 3 SampleID, Day, Description

\(darkgreen\)heat \(darkgreen\)heat_rel \(darkgreen\)tree \(darkgreen\)taxa

	Kingdom	Phylum	Class	Order	Family	Genus	Species
Seq92	k__Fungi	p__Basidiomycota	c__Agaricomycetes	o__Agaricales	f__Bolbitiaceae	g__Conocybe	s__zeylanica
Seq49	k__Fungi	p__Basidiomycota	c__Agaricomycetes	o__Agaricales	f__Bolbitiaceae	g__Conocybe	s__zeylanica
Seq91	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Coniochaetales	f__Coniochaetaceae	g__Coniochaeta	s__canina
Seq6	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Chaetomiaceae	g__Humicola	s__sardiniae
Seq16	k__Fungi	p__Basidiomycota	c__Agaricomycetes	o__Agaricales	f__Bolbitiaceae	g__Conocybe	s__zeylanica
Seq67	k__Metazoa	p__Annelida	c__Clitellata	o__Enchytraeida	f__Enchytraeidae	g__Fridericia	NA
Seq13	k__Fungi	p__Ascomycota	c__Eurotiomycetes	o__Eurotiales	f__Trichocomaceae	g__Talaromyces	NA
Seq7	k__Fungi	p__Basidiomycota	c__Agaricomycetes	o__Agaricales	f__Bolbitiaceae	g__Conocybe	s__zeylanica
Seq25	k__Fungi	p__Basidiomycota	c__Cystobasidiomycetes	o__Cystobasidiales	f__Cystobasidiaceae	g__Occultifur	NA
Seq69	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Nectriaceae	g__Gibberella	s__intricans
Seq86	k__Fungi	p__Basidiomycota	c__Agaricomycetes	o__Agaricales	f__Bolbitiaceae	g__Conocybe	s__zeylanica
Seq205	k__Fungi	p__Mucoromycota	c__Mucoromycetes	o__Mucorales	f__Rhizopodaceae	g__Rhizopus	s__arrhizus
Seq50	k__Fungi	p__Basidiomycota	c__Cystobasidiomycetes	o__Cystobasidiales	f__Cystobasidiaceae	g__Occultifur	NA
Seq193	k__Heterolobosa	p__Heterolobosa_phy_Incertae_sedis	c__Heterolobosea	o__Schizopyrenida	f__Vahlkampfiidae	g__Naegleria	NA
Seq136	k__Fungi	p__Ascomycota	c__Eurotiomycetes	o__Chaetothyriales	f__Herpotrichiellaceae	g__Exophiala	NA
Seq23	k__Fungi	p__Mucoromycota	c__Mucoromycetes	o__Mucorales	f__Mucoraceae	g__Actinomucor	s__elegans
Seq175	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Bionectriaceae	g__Clonostachys	s__rosea
Seq281	k__Heterolobosa	p__Heterolobosa_phy_Incertae_sedis	c__Heterolobosea	o__Schizopyrenida	f__Vahlkampfiidae	g__Allovahlkampfia	NA
Seq47	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Nectriaceae	g__Gibberella	s__intricans
Seq137	k__Fungi	p__Ascomycota	c__Dothideomycetes	o__Venturiales	f__Sympoventuriaceae	g__Ochroconis	s__tshawytschae
Seq9	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Stachybotryaceae	g__Albifimbria	s__verrucaria
Seq42	k__Fungi	p__Basidiomycota	c__Agaricomycetes	o__Agaricales	f__Bolbitiaceae	g__Conocybe	s__zeylanica
Seq45	k__Fungi	NA	NA	NA	NA	NA	NA
Seq11	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Nectriaceae	g__Gibberella	s__intricans
Seq89	k__Fungi	p__Basidiomycota	c__Cystobasidiomycetes	o__Cystobasidiales	f__Cystobasidiaceae	g__Occultifur	NA
Seq171	k__Fungi	p__Ascomycota	c__Dothideomycetes	o__Venturiales	f__Sympoventuriaceae	g__Ochroconis	s__tshawytschae
Seq178	k__Fungi	p__Ascomycota	c__Dothideomycetes	o__Venturiales	f__Sympoventuriaceae	g__Scolecobasidium	s__constrictum
Seq22	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Coniochaetales	f__Coniochaetaceae	g__Coniochaeta	s__verticillata
Seq31	k__Fungi	p__Mucoromycota	c__Mucoromycetes	o__Mucorales	f__Mucoraceae	g__Actinomucor	s__elegans
Seq65	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Coniochaetales	f__Coniochaetaceae	g__Coniochaeta	NA
Seq75	NA	NA	NA	NA	NA	NA	NA
Seq85	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA
Seq54	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Coniochaetales	f__Coniochaetaceae	g__Coniochaeta	NA
Seq121	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Nectriaceae	g__Gibberella	s__intricans
Seq125	k__Fungi	p__Ascomycota	c__Dothideomycetes	o__Venturiales	f__Sympoventuriaceae	g__Ochroconis	s__tshawytschae
Seq181	k__Fungi	p__Ascomycota	c__Eurotiomycetes	o__Eurotiales	f__Aspergillaceae	g__Penicillium	s__bialowiezense
Seq24	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Nectriaceae	g__Gibberella	s__intricans
Seq27	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Stachybotryaceae	g__Stachybotrys	s__chartarum
Seq66	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Stachybotryaceae	g__Albifimbria	s__verrucaria
Seq114	k__Fungi	p__Basidiomycota	c__Agaricomycetes	o__Agaricales	f__Bolbitiaceae	g__Conocybe	s__zeylanica
Seq15	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Nectriaceae	g__Gibberella	s__intricans
Seq226	k__Fungi	p__Ascomycota	c__Dothideomycetes	o__Venturiales	f__Sympoventuriaceae	g__Scolecobasidium	s__constrictum
Seq26	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Chaetomiaceae	g__Chaetomium	s__iranianum
Seq59	k__Fungi	p__Ascomycota	c__Dothideomycetes	o__Venturiales	f__Sympoventuriaceae	g__Ochroconis	s__tshawytschae
Seq41	k__Fungi	p__Basidiomycota	c__Agaricomycetes	o__Cantharellales	f__Ceratobasidiaceae	g__Waitea	s__circinata
Seq157	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Chaetomiaceae	g__Zopfiella	NA
Seq124	k__Fungi	p__Ascomycota	c__Eurotiomycetes	o__Eurotiales	f__Aspergillaceae	g__Aspergillus	NA
Seq254	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA
Seq17	k__Metazoa	p__Nematoda	NA	NA	NA	NA	NA
Seq39	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Coniochaetales	f__Coniochaetaceae	g__Coniochaeta	s__verticillata
Seq180	NA	NA	NA	NA	NA	NA	NA
Seq20	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Chaetomiaceae	g__Chaetomium	NA
Seq102	NA	NA	NA	NA	NA	NA	NA
Seq70	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Sordariales_fam_Incertae_sedis	g__Staphylotrichum	s__boninense
Seq150	k__Heterolobosa	p__Heterolobosa_phy_Incertae_sedis	c__Heterolobosea	o__Schizopyrenida	f__Vahlkampfiidae	g__Allovahlkampfia	NA
Seq132	NA	NA	NA	NA	NA	NA	NA

$salmon \(salmon\)ps phyloseq-class experiment-level object otu_table() OTU Table: [ 24 taxa and 36 samples ] sample_data() Sample Data: [ 36 samples by 2 sample variables ] tax_table() Taxonomy Table: [ 24 taxa by 7 taxonomic ranks ] refseq() DNAStringSet: [ 24 reference sequences ]

\(salmon\)amp ampvis2 object with 4 elements. Summary of OTU table: Samples OTUs Total#Reads Min#Reads Max#Reads Median#Reads 36 24 121335 20 14955 1772 Avg#Reads 3370.42

Assigned taxonomy: Kingdom Phylum Class Order Family Genus Species 22(92%) 18(75%) 15(62.5%) 14(58.33%) 13(54.17%) 13(54.17%) 11(45.83%)

Metadata variables: 3 SampleID, Day, Description

\(salmon\)heat \(salmon\)heat_rel \(salmon\)tree \(salmon\)taxa

	Kingdom	Phylum	Class	Order	Family	Genus	Species
Seq3	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Lasiosphaeriaceae	g__Schizothecium	s__inaequale
Seq19	k__Fungi	p__Basidiomycota	c__Agaricomycetes	o__Agaricales	f__Psathyrellaceae	g__Coprinellus	s__flocculosus
Seq53	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Stachybotryaceae	g__Albifimbria	s__verrucaria
Seq99	k__Viridiplantae	p__Anthophyta	NA	NA	NA	NA	NA
Seq215	k__Viridiplantae	NA	NA	NA	NA	NA	NA
Seq30	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Chaetomiaceae	g__Humicola	s__sardiniae
Seq43	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Lasiosphaeriaceae	g__Schizothecium	NA
Seq68	NA	NA	NA	NA	NA	NA	NA
Seq63	k__Fungi	p__Mucoromycota	c__Mucoromycetes	o__Mucorales	f__Mucoraceae	g__Actinomucor	s__elegans
Seq100	k__Fungi	NA	NA	NA	NA	NA	NA
Seq140	k__Fungi	p__Ascomycota	c__Dothideomycetes	o__Pleosporales	NA	NA	NA
Seq33	k__Fungi	p__Basidiomycota	c__Agaricomycetes	o__Agaricales	f__Psathyrellaceae	g__Coprinellus	s__flocculosus
Seq245	NA	NA	NA	NA	NA	NA	NA
Seq138	k__Viridiplantae	p__Anthophyta	c__Eudicotyledonae	NA	NA	NA	NA
Seq8	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Lasiosphaeriaceae	g__Schizothecium	s__inaequale
Seq79	k__Viridiplantae	p__Anthophyta	NA	NA	NA	NA	NA
Seq161	k__Eukaryota_kgd_Incertae_sedis	NA	NA	NA	NA	NA	NA
Seq96	k__Metazoa	p__Nematoda	c__Chromadorea	o__Rhabditida	f__Cephalobidae	g__Acrobeloides	s__nanus
Seq147	k__Fungi	NA	NA	NA	NA	NA	NA
Seq123	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Chaetomiaceae	g__Chaetomium	s__jodhpurense
Seq152	k__Viridiplantae	p__Anthophyta	NA	NA	NA	NA	NA
Seq52	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Sordariales	f__Lasiosphaeriaceae	g__Schizothecium	NA
Seq40	k__Fungi	p__Mucoromycota	c__Mucoromycetes	o__Mucorales	f__Mucoraceae	g__Actinomucor	s__elegans
Seq37	k__Fungi	p__Ascomycota	c__Sordariomycetes	o__Hypocreales	f__Nectriaceae	g__Gibberella	s__intricans

sample.data <- ps_its@sam_data %>% 
  data.frame() %>% 
  mutate(Group = if_else(Day %in% c("D01", "D03", "D05"), "early", 
                         if_else(Day %in% c("D07", "D08","D10"), "middle", "late"))) %>% 
  mutate(Group = factor(Group, levels=c("early", "middle","late"))) %>% 
  phyloseq::sample_data()

sample_data(ps_its) <- sample.data

p.observed <- plot_alpha_w_toc(ps = ps_its, group = "Group", metric = c("Observed")) + 
  theme(axis.title.y = element_blank())

p.shannon <- plot_alpha_w_toc(ps = ps_its, group = "Group", metric = c("Shannon")) + 
  theme(axis.title.y = element_blank())

p.simpson <- plot_alpha_w_toc(ps = ps_its, group = "Group", metric = c("InvSimpson")) + 
  theme(axis.title.y = element_blank())

ggpubr::ggarrange(p.observed, p.shannon, p.simpson, ncol = 3)

p.observed <- plot_alpha_w_toc(ps = ps_its, group = "Day", metric = c("Observed")) + 
  theme(axis.title.y = element_blank())

p.shannon <- plot_alpha_w_toc(ps = ps_its, group = "Day", metric = c("Shannon")) + 
  theme(axis.title.y = element_blank())

p.simpson <- plot_alpha_w_toc(ps = ps_its, group = "Day", metric = c("InvSimpson")) + 
  theme(axis.title.y = element_blank())

ggpubr::ggarrange(p.observed, p.shannon, p.simpson, ncol = 3)

ps.m <- phyloseq::psmelt(ps_its)
ps.m <- ps.m %>% 
  mutate_if(is.character, as.factor) 

ps.data.out <- ps.m %>%
  select(-Group) %>% 
  pivot_wider(names_from = c(Day, Description, Sample), values_from = Abundance, values_fill = 0) 

#create empty dataframe with columnnames
external_empty_dataframe <- data.frame(OTU=factor(), cluster=factor(), stringsAsFactors = FALSE)

for (i in names(l_its)) {
  a <- taxa_names(l_its[[i]][["ps"]])
  b <- rep(i, length(a))
  d <- data.frame(OTU = as.factor(a),
                  cluster = as.factor(b))
  external_empty_dataframe <- rbind(external_empty_dataframe, d)
}

clusters.otu.df <- external_empty_dataframe
# add exl taxa -- taxa "exl"
d <- data.frame(OTU = as.factor(ps.its.exl.taxa),
                cluster = as.factor(rep("exl", length(ps.its.exl.taxa))))

clusters.otu.df <- rbind(clusters.otu.df, d)
ps.data.out.exl <- left_join(clusters.otu.df, ps.data.out, by="OTU")
# write.table(ps.data.out.exl, file = "ps.its.data.out.tsv", sep = "\t")


ps.data.out.exl